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EC number: 267-636-0 | CAS number: 67905-17-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Data is from experimental study report
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- other: EC Directive 92/69/EC Method C.2 (1992)
- GLP compliance:
- yes
- Remarks:
- Deviation from GLP principles - at the sponsor’s request no test item analysis was carried out.
- Analytical monitoring:
- no
- Remarks:
- At the sponsor’s request no test item analysis was carried out.
- Vehicle:
- no
- Details on test solutions:
- The solution (100 mg/L test chemical was weighed out) was prepared with dilution water one day prior to application in the test medium. The solubility of test substance is < 1 mg/L (related to the pigment). The stock solution was shaken at room temperature with 20 rpm for 24 h (rotating shaker 3040, GFL) and membrane filtrated with 0.45 μm
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: STRAUS
- Culture: In 2-3 L glass vessels with approximatively 1.8 L culture medium, at 21 °C (temperatures of 20 - 25 °C are tolerated), in an incubator, 16 h illumination, illumination strength max. 20 μE.m-2.s-1.
- Culture media: Culture medium Elendt M4, according to Elendt (1990), modified to a total hardness of 160 to 180 mg CaCO3/L.
- Strain/clone: Clone 5
- Source: Institut für Wasser-, Boden- und Lufthygiene (WaBoLu)
- Age of parental stock (mean and range, SD): 2 - 24 h old
- Feeding during test: The daphnids were not fed during the study.
ACCLIMATION
- Acclimation period: Ca. 2 h
- Type and amount of food: The daphnids were fed with Desmodesmus subspicatus and Chlorella vulgaris during the study.
- Food type: Desmodesmus subspicatus and Chlorella vulgaris with an algae cell density of > 106 cells/mL.
- Amount: ad libitum
- Frequency: Atleast 5x weekly
- Health during acclimation (any mortality observed):
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 266 mg CaCO3/L
- Test temperature:
- 18 - 22 °C, ± 1 °C constant
- pH:
- 7.80
- Dissolved oxygen:
- 8.69 mg/L
- Conductivity:
- 658 μS/cm
- Nominal and measured concentrations:
- Saturated solution (100 mg/L)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers were used as a test vessel.
- fill volume: 50 ml
- Volume of the study 20 mL medium
- Number of study organisms: 20 animals, divided into 4 (2*) parallel samples, each with 5 (10*) animals
- No. of vessels per concentration (replicates): 4 replicates for test conc.. and 2 replicates for reference substance.
- Illumination : Diffuse light, illumination range max. 20 20 μE.m-2 .s-1
- Photoperiod : 16/8 h light/dark cycle
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: According to EC 92/69 L383A C.2. Annex. Components of the dilution water includes KCl, NaHCO3, CaCl2.2H2O, MgSO4.7H2O - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: At the saturated solution (100 mg/L) of the test item has no biologically significant effect was determined
- Results with reference substance (positive control):
- For , referance substance
EC10 : 1.51
EC50 : 1.84 (CI 1.74 - 1.95)
EC100 : 3.20 - Reported statistics and error estimates:
- EC10 and EC50 values were only calculated for the reference item via probit according to WEBER (1986). Calculation of the confidence intervals were carried out using standard procedures according to BREITIG & TÜMPLING (1982).
- Validity criteria fulfilled:
- yes
- Conclusions:
- The median effect concentration (EC50) of saturated solution of test chemical was observed to be >100 mg/l after 48 hrs of exposure period.
- Executive summary:
Acute Immobilisation Test for 48 h in static methode to Daphnia magna STRAUS was performed according to OECD-Guideline 202 for Testing of Chemicals (2004) EC Directive 92/69/EC Method C.2 (1992). The solution (100 mg/L test chemical was weighed out) was prepared with dilution water one day prior to application in the test medium. The solubility of test substance is < 1 mg/L (related to the pigment). The stock solution was shaken at room temperature with 20 rpm for 24 h (rotating shaker 3040, GFL) and membrane filtrated with 0.45 μm .The effect of the saturated solution (100 mg/L) as limit concentration of the test chemical was determined.The limit test was conducted under static conditions over 48 h . 20 test organisms were exposed to the limit concentration and control. No test chemical analysis was carried out. A reference test was carried out with potassium dichromate to determine the toxicity of the reference substance. EC10 and EC50 values were only calculated for the reference chemical via probit according to WEBER (1986). Calculation of the confidence intervals were carried out using standard procedures according to BREITIG & TÜMPLING (1982). The median effect concentration (EC50) of saturated solution of test chemical was observed to be >100 mg/l after 48 hrs of exposure period.
Reference
Table 1: Percentage Number of Daphnia Incapable of Swimming after 24 and 48 h of Exposure (n = 20)
IMMOBILISATION [%] |
||||||||||
|
24 h |
48 h |
||||||||
Replicates |
Replicates |
|||||||||
|
1 |
2 |
3 |
4 |
MV |
1 |
2 |
3 |
4 |
MV |
Saturated solution (100 mg/L) |
0 |
0 |
|
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Control |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Description of key information
Acute Immobilisation Test for 48 h in static method to Daphnia magna STRAUS was performed according to OECD-Guideline 202 for Testing of Chemicals (2004) EC Directive 92/69/EC Method C.2 (1992) (Experimental study report, 2005). The solution (100 mg/L test chemical was weighed out) was prepared with dilution water one day prior to application in the test medium. The solubility of test substance is < 1 mg/L (related to the pigment). The stock solution was shaken at room temperature with 20 rpm for 24 h (rotating shaker 3040, GFL) and membrane filtrated with 0.45 μm .The effect of the saturated solution (100 mg/L) as limit concentration of the test chemical was determined. The limit test was conducted under static conditions over 48 h. 20 test organisms were exposed to the limit concentration and control. No test chemical analysis was carried out. A reference test was carried out with potassium dichromate to determine the toxicity of the reference substance. EC10 and EC50 values were only calculated for the reference chemical via probit according to WEBER (1986). Calculation of the confidence intervals were carried out using standard procedures according to BREITIG & TÜMPLING (1982). The median effect concentration (EC50) of saturated solution of test chemical was observed to be >100 mg/l after 48 hrs of exposure period.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
Acute Immobilisation Test for 48 h in static method to Daphnia magna STRAUS was performed according to OECD-Guideline 202 for Testing of Chemicals (2004) EC Directive 92/69/EC Method C.2 (1992) (Experimental study report, 2005). The solution (100 mg/L test chemical was weighed out) was prepared with dilution water one day prior to application in the test medium. The solubility of test substance is < 1 mg/L (related to the pigment). The stock solution was shaken at room temperature with 20 rpm for 24 h (rotating shaker 3040, GFL) and membrane filtrated with 0.45 μm .The effect of the saturated solution (100 mg/L) as limit concentration of the test chemical was determined. The limit test was conducted under static conditions over 48 h. 20 test organisms were exposed to the limit concentration and control. No test chemical analysis was carried out. A reference test was carried out with potassium dichromate to determine the toxicity of the reference substance. EC10 and EC50 values were only calculated for the reference chemical via probit according to WEBER (1986). Calculation of the confidence intervals were carried out using standard procedures according to BREITIG & TÜMPLING (1982). The median effect concentration (EC50) of saturated solution of test chemical was observed to be >100 mg/l after 48 hrs of exposure period.
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