Reaction mass of fatty acids, montan-wax, 1-methyl-1,3- propanediyl esters and fatty acids, montan-wax, ethylene esters and fatty

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

migrated information: read-across based on grouping of substances (category approach)

Adequacy of study:

key study

Study period:

From MAR 20 2002 to APR 28 2002

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study performed according to relevant guideline and compliant to GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Montan waxes, type CaV 102

IUPAC Name:

Montan waxes, type CaV 102

Details on test material:

"Licomont CaV 102", calcium salt of montanacids
active content: 100 %

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

Secondary effluent of domestic sewage treatment plant. Filtrate of activated sludge tank.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

ca. 1 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

All measurements were performed in duplicate:

Dilution water: deionized (distillate) water
Mineral medium: Mix of different minerals and deionized water - stock solutions:
(a) 8.5 g of KH2PO4 , 21.75g of K2HPO4 , 33.4 g of Na2HPO4*2H20 and 0.5 g NH4CI made up to 1 L;
(b) 36.4 g of CaCl2*2H20 made up to 1 L
(c) 22.5 g of MgSO4*7H20 made up to 1 L
(d) 0.25 g of FeCI3*H2O made up to 1 L
Preparation: Adding 1 mL of solutions (a), (b), (c) and (d) to 800 mL dilution water, this was made up to 1 L .
Apparatus: BOD bottles, Oxygen electrode and meter, large glass bottles, graduated cylinder, thermometer, pipette;

Procedure:
a) Preparation of bottles
The preparation of the stock solution was set up by a weighted portion of about 40 mg from the test substance which was made up to 100 ml with distillate water (stock solution).
The mineral medium was strongly aerated for at least 30 minutes and was allowed to stand for 20 h at the test temperature (02-concentration was about 9 mg/L at 20 deg C).
Then there was added about 1.5 L fully-aerated mineral medium to a large bottle (4 L) so that it was about one-third full. Afterwards there was added an appropriate amount (about 40 mL) of the stock solution of the test substance to reach the final concentration of 4 mg/L (between 2 and 5) and inoculated with secondary effluent. Finally, it was made up the solution to final volume of 4L with aerated mineral medium.
The same procedure was repeated to prepare the flasks for the reference compound.
Each test series consisted of:
- about 10 bottles containing test suspension (test substance and inoculum)
- about 10 bottles containing inoculum alone (blank inoculum)
- about 10 bottles containing reference compound and inoculum (procedure control)

b) Test procedure
Afterwards the prepared solution was dispensed immediately into the BOD bottles by hose from the lower quarter of the appropriate large bottles, so that all BOD bottles were completely filled. After analyzing the zero-time bottles for dissolved oxygen the other ones were incubated in dark at constant temperature (about 20 °C).
To allow the assessment of percentage removal in a 14 d (10 d) window observations of dissolved oxygen were carried out at days 1, 2, 5, 12, 17, 23 and 28.
All test series were running in parallel. The reference substance was Propylenalkylbenzolsulfonate with a known biological degradation rate (about 30 % in Zahn-Wellens test for inherent biodegradability according to OECD 302B).

Reference substance

Reference substance:

other: propylene alkylbenzene sulfonate, cross-linked

Results and discussion

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

26.1

Sampling time:

28 d

Details on results:

ThOD submission substance: 3.92 mg O2/mg;
ThOD reference substance: 2.84 mg O2/mg;
Pleas see table section "Any other iformation on results incl. tables" for further details on results!

BOD5 / COD results

Results with reference substance:

Biological degradation (BOD/ThOD x 100%) of the reference substance at day 28 was 35.8%. This was expected (known biological degradation rate in Zahn-Wellens-Test according to OECD 302B was about 30%).

Any other information on results incl. tables

Details on degradation values:

Days	Specific Oxygendepletion (BOD)	Specific Oxygendepletion (BOD)	Biological degradation	Biological degradation
	Test substance	Reference substance	Test substance	Reference substance
d	mg/mg	mg/mg	%	%
1	0.031	0.047	0.8	1.6
2	0.075	0.157	1.9	5.5
5	0.289	0.384	7.4	13.5
12	0.536	0.717	13.7	25.3
17	0.717	0.857	18.3	30.2
23	0.976	0.892	24.9	31.4
28	1.023	0.917	26.1	32.3

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other: Under test conditions not readily biodegradable

Conclusions:

The submission substance was tested for ready biodegradability according to OECD 301 D (closed bottle test). Under test conditions the substance was not readily biodegradable.

Executive summary:

The submission substance was tested for ready biodegradability according to OECD 301 D (closed bottle test) and compliant to GLP (reliability category 1). At day 28, 26.1 % biodegradation was reached. Therefore, according to these results the submission substance is not readily biodegradable.