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EC number: 813-130-5 | CAS number: 4039-86-5
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 2014-12-01 to 12015-03-23
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Experimental study according to guideline and GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 437 (Bovine Corneal Opacity and Permeability Test Method for Identifying Ocular Corrosives and Severe Irritants)
- Version / remarks:
- 2013
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Commission Regulation (EU) No. 1152/2010 of 8 December 2010 Part B: Methods for the det.: Bovine Corneal Opacity and Permeability Test, Method for identifying ocular corrosives and severe irritants; Official Journal of the European Union, No. L 324 (B.47?
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- BASF SE, Experimental Toxicology and Ecology, 67056 Ludwigshafen, Germany
Test material
- Reference substance name:
- 1-chloro-4-[(prop-2-yn-1-yloxy)methyl]benzene
- EC Number:
- 813-130-5
- Cas Number:
- 4039-86-5
- Molecular formula:
- C10H9ClO
- IUPAC Name:
- 1-chloro-4-[(prop-2-yn-1-yloxy)methyl]benzene
Constituent 1
Test system
- Vehicle:
- unchanged (no vehicle)
- Amount / concentration applied:
- TEST MATERIAL
- Amount applied: 750 µL
- Concentration: undiluted - Duration of treatment / exposure:
- 10 min
- Details on study design:
- Please refer to "Any other information on materials and methods"
Results and discussion
In vitro
Resultsopen allclose all
- Irritation parameter:
- in vitro irritation score
- Run / experiment:
- 10 min exposure, 2 h post-exposure (test substance)
- Value:
- 0.5
- Irritation parameter:
- in vitro irritation score
- Run / experiment:
- 10 min exposure, 2 h post-exposure (negative control)
- Value:
- 1.9
- Irritation parameter:
- in vitro irritation score
- Run / experiment:
- 10 min exposure, 2 h post-exposure (positive control, ethanol 100 %)
- Value:
- 63.3
- Irritation parameter:
- in vitro irritation score
- Run / experiment:
- 10 min exposure, 2 h post-exposure (positive control, Dimethylformamid 100 %)
- Value:
- 129
Any other information on results incl. tables
Opacity
Substance |
Cornea No. |
Initial opacity |
Final opacity |
Opacity change |
Corrected opacity change |
Mean |
SD |
Test substance |
10 |
2.5 |
5.5 |
3 |
1.1 |
0.5 |
0.6 |
11 |
2.8 |
5.1 |
2.3 |
0.5 |
|||
12 |
4.6 |
5.9 |
1.3 |
0.0 |
|||
Negative control |
1 |
1.7 |
2.3 |
0.7 |
NA |
1.8 |
2.6 |
2 |
4.4 |
3.4 |
0.0 |
NA |
|||
3 |
4.3 |
9.1 |
4.9 |
NA |
|||
Ethanol (100 %) |
4 |
5.6 |
36.8 |
31.2 |
29.3 |
32.4 |
3.5 |
5 |
3.7 |
37.2 |
33.6 |
31.7 |
|||
6 |
2.1 |
40.1 |
38.0 |
36.2 |
|||
Dimethylformamid (100 %) |
7 |
2.2 |
95.9 |
93.7 |
91.8 |
104.0 |
13.2 |
8 |
4.0 |
123.8 |
119.8 |
118.0 |
|||
9 |
2.7 |
106.8 |
104.1 |
102.2 |
Permeability
Substance |
Cornea No. |
Mean OD490 |
Dilution factor |
Mean corrected OD490 |
Mean |
SD |
Test substance |
10 |
0.001 |
1 |
0.001 |
0.001 |
0.001 |
11 |
0.002 |
1 |
0.002 |
|||
12 |
-0.002 |
1 |
0 |
|||
Negative control |
1 |
-0.002 |
1 |
NA |
0 |
0 |
2 |
0 |
1 |
NA |
|||
3 |
0 |
1 |
NA |
|||
Ethanol (100 %) |
4 |
0.446 |
5 |
2.231 |
2.059 |
0.318 |
5 |
0.338 |
5 |
1.691 |
|||
6 |
0.451 |
5 |
2.253 |
|||
Dimethylformamid (100 %) |
7 |
0.172 |
5 |
0.860 |
1.665 |
0.736 |
8 |
0.461 |
5 |
2.305 |
|||
9 |
0.366 |
5 |
1.830 |
In Vitro Irritancy score (IVIS)
Substance |
Cornea No. |
Opacity per cornea |
Permeability per cornea |
IVIS |
||
per cornea |
per group |
|||||
Mean |
SD |
|||||
Test substance |
10 |
1.1 |
0.001 |
1.1 |
0.5 |
0.6 |
11 |
0.5 |
0.002 |
0.5 |
|||
12 |
0 |
0 |
0 |
|||
Negative control |
1 |
0.7 |
0 |
0.7 |
1.9 |
2.6 |
2 |
0 |
0 |
0 |
|||
3 |
4.9 |
0.001 |
4.9 |
|||
Ethanol (100 %) |
4 |
29.3 |
2.231 |
62.8 |
63.3 |
6.5 |
5 |
31.7 |
1.691 |
57.1 |
|||
6 |
36.2 |
2.253 |
70.0 |
|||
Dimethylformamid (100 %) |
7 |
91.8 |
0.860 |
104.7 |
129.0 |
23.9 |
8 |
118.0 |
2.305 |
152.5 |
|||
9 |
102.2 |
1.830 |
139.7 |
|||
Applicant's summary and conclusion
- Interpretation of results:
- other: not irritating and not corrosive
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