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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Justification for type of information:
A scientific publication, method similar to OECD 471 with deviations.

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1984

Materials and methods

Principles of method if other than guideline:
Study design is comparable to OECD 471, the data does not meet important criteria of current standard methods.
The indicator organisms were Saccharomyces cerevisiae strain D4 and S. typhimurium strains TA1535, TA1537, and TA1538.
GLP compliance:
no
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
Sodium metaphosphate
EC Number:
233-343-1
EC Name:
Sodium metaphosphate
Cas Number:
10124-56-8
Molecular formula:
Na6O18P6
IUPAC Name:
hexasodium 2,4,6,8,10,12-hexaoxocyclohexaphosphoxane-2,4,6,8,10,12-hexakis(olate)

Method

Species / strainopen allclose all
Species / strain / cell type:
S. typhimurium TA 1535
Additional strain / cell type characteristics:
not applicable
Species / strain / cell type:
S. typhimurium TA 1537
Additional strain / cell type characteristics:
not applicable
Species / strain / cell type:
S. typhimurium TA 1538
Additional strain / cell type characteristics:
not applicable
Species / strain / cell type:
Saccharomyces cerevisiae
Additional strain / cell type characteristics:
other:
Remarks:
Saccharomyces cerevisiae strain D4
Species / strain / cell type:
Saccharomyces cerevisiae
Additional strain / cell type characteristics:
other: Saccharomyces cerevisiae strain S.
Metabolic activation:
with and without

Results and discussion

Any other information on results incl. tables

Sodium Hexametaphosphate was not mutagenic for S. typhimurium strains TA1535, TA1537, or TA1538 in direct or activation plate tests.

Test results were negative for the nonactivation suspension tests. Initially, test results from the activation suspension assays using rat tissues and strain TA1538 were unusually high, but repeat data indicated that “the original tests were aberrant and did not reflect true mutagenic activity.” In addition, the suspension test using rat testes and strain TA1537 at the high-dose concentration (with activation) had unusually high results, but other data did not support the response; the difference was considered either a random test fluctuation or the result of contaminants.

For suspension assays using S. cerevisiae as the test organism, the results were negative with and without activation.

Sodium hexametaphosphate was not considered mutagenic under the conditions of this study.

Applicant's summary and conclusion

Conclusions:
Sodium Hexametaphosphate was not mutagenic for S. typhimurium strains TA1535, TA1537, or TA1538 in direct or activation plate tests.
Executive summary:

Sodium Hexametaphosphate was not mutagenic for S. typhimurium strains TA1535, TA1537, or TA1538 in direct or activation plate tests.