Sodium benzothiazol-2-yl sulphide

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro cytogenicity / chromosome aberration study in mammalian cells

Remarks:

Type of genotoxicity: chromosome aberration

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: acceptable documented publication

Data source

Reference

Reference Type:

publication

Title:

In vitro induction of polyploidy and chromatid exchanges by culture medium extracts of natural rubbers compounded with 2-mrcaptobenzothiazole as a positve control candidate for genotoxicity tests

Author:

Matsuoka, A.; et al.

Year:

2005

Bibliographic source:

J. Biomed. Mater. Res. 75A, 439-444

Materials and methods

Principles of method if other than guideline:

in vitro chromosome aberration assay

GLP compliance:

not specified

Type of assay:

in vitro mammalian chromosome aberration test

Test material

Test material information

Constituent 1

Reference substance name:

Benzothiazole-2-thiol

EC Number:

205-736-8

EC Name:

Benzothiazole-2-thiol

Cas Number:

149-30-4

Molecular formula:

C7H5NS2

IUPAC Name:

1,3-benzothiazole-2-thiol

Details on test material:

MBT

Method

Target gene:

chromosome aberration and endoreduplication

Species / strain

Species / strain / cell type:

mammalian cell line, other: CHL cells

Metabolic activation:

with and without

Metabolic activation system:

S9-mix

Test concentrations with justification for top dose:

-S9: 0, 0.2, 0.4 µg/ml, +S9: 0, 0.2, 0.4, 0.6 µg/ml

Controls

Untreated negative controls:

not specified

Negative solvent / vehicle controls:

yes

True negative controls:

not specified

Positive controls:

yes

Positive control substance:

other: additional substances tested, like extracts from rubber sheets, which induced significant increase in abberant cells compared to solvent control

Results and discussion

Test results

Species / strain:

mammalian cell line, other: CHL cells

Metabolic activation:

with and without

Genotoxicity:

other: chromosome aberration: -S9 negative, +S9 inconclusive; increase in polyploid cells and endoreduplications

Cytotoxicity / choice of top concentrations:

cytotoxicity

Vehicle controls validity:

valid

Untreated negative controls validity:

not specified

Positive controls validity:

other: number of aberrant cells increased after treatment with rubber extracts

Remarks on result:

other: strain/cell type: CHL cells

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

In the CA test performed with CHL cells, MBT induced polyploidy, including endoreduplication, in the absence and presence of S9 mix. Frequency of polyploid cells and endoreduplications was 3.6% and 6.2% without S9 mix and 2 % and 0.4 % with S9 mix at 0.2 mg/ml. No endoreduplications were noted in the solvent control.

MBT showed inconclusive response in structural CA induction at 0.4 mg/ml with S9 mix.