Registration Dossier

Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
10 Mar - 7 Apr 1993
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1993
Report date:
1993

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
Deviations:
no
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
1,4:3,6-dianhydro-2,5-di-O-methyl-D-glucitol
EC Number:
226-159-8
EC Name:
1,4:3,6-dianhydro-2,5-di-O-methyl-D-glucitol
Cas Number:
5306-85-4
Molecular formula:
C8H14O4
IUPAC Name:
1,4:3,6-dianhydro-2,5-di-O-methyl-D-glucitol

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge: Buckland Sewage Treatment Works, Newton Abbot, Devon. Sludge was collected on 1st March 1993.
- Preparation of inoculum for exposure: the sludge was aerated at 20 ± 3 °C overnight. 500 mL were washed with test medium, centrifuged and resuspended in test medium.
- Concentration of sludge: the inoculated test medium was prepared by making 99 mL of the washed sludge up to 10 L in test medium.
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
TEST CONDITIONS
- Composition of medium: OECD guideline medium
- Test temperature: 20 ± 2 °C
- pH: not stated
- Aeration of dilution water: yes
- Suspended solids concentration: 3364 mg/L
- Continuous darkness: yes

TEST SYSTEM
- Culturing apparatus: 500 mL dark glass bottles placed on magnetic stirrer and connected to a closed end Hg manometer
- Number of culture flasks/concentration: 3
- Measuring equipment: oxygen uptake: modified Hach manometric BOD apparatus (Hach Chemical Co, Ames, Iowa, US)
- Test performed in open system: no

SAMPLING
Oxygen uptake was recorded on day 5, 10, 15, 20, 25 and 28

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, three bottles
- Abiotic sterile control: no
- Toxicity control: no
- Reference substance: yes, three bottles
Reference substance
Reference substance:
acetic acid, sodium salt
Remarks:
initial concentration: 200 mg/L

Results and discussion

% Degradationopen allclose all
Parameter:
% degradation (O2 consumption)
Value:
< 1
Sampling time:
28 d
Parameter:
% degradation (DOC removal)
Value:
14.1
Sampling time:
28 d

BOD5 / COD results

Results with reference substance:
78% after 28 days

Any other information on results incl. tables

Biodegradation rate based on oxygen consumption:

Day Biodegradation rate [%]
Blank Test substance Reference substance
5 4.3 0 67
10 5.2 0 76
15 5.7 0 78
20 5.7 0 78
25 5.7 0 78
28 5.7 0 78

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed

Categories Display