1,4:3,6-dianhydro-2,5-di-O-methyl-D-glucitol

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

10 Mar - 7 Apr 1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1,4:3,6-dianhydro-2,5-di-O-methyl-D-glucitol

EC Number:

226-159-8

EC Name:

1,4:3,6-dianhydro-2,5-di-O-methyl-D-glucitol

Cas Number:

5306-85-4

Molecular formula:

C8H14O4

IUPAC Name:

1,4:3,6-dianhydro-2,5-di-O-methyl-D-glucitol

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge: Buckland Sewage Treatment Works, Newton Abbot, Devon. Sludge was collected on 1st March 1993.
- Preparation of inoculum for exposure: the sludge was aerated at 20 ± 3 °C overnight. 500 mL were washed with test medium, centrifuged and resuspended in test medium.
- Concentration of sludge: the inoculated test medium was prepared by making 99 mL of the washed sludge up to 10 L in test medium.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium: OECD guideline medium
- Test temperature: 20 ± 2 °C
- pH: not stated
- Aeration of dilution water: yes
- Suspended solids concentration: 3364 mg/L
- Continuous darkness: yes

TEST SYSTEM
- Culturing apparatus: 500 mL dark glass bottles placed on magnetic stirrer and connected to a closed end Hg manometer
- Number of culture flasks/concentration: 3
- Measuring equipment: oxygen uptake: modified Hach manometric BOD apparatus (Hach Chemical Co, Ames, Iowa, US)
- Test performed in open system: no

SAMPLING
Oxygen uptake was recorded on day 5, 10, 15, 20, 25 and 28

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, three bottles
- Abiotic sterile control: no
- Toxicity control: no
- Reference substance: yes, three bottles

Reference substance

Reference substance:

acetic acid, sodium salt

Remarks:

initial concentration: 200 mg/L

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (O2 consumption)

Value:

< 1

Sampling time:

28 d

% Degradation 2

Parameter:

% degradation (DOC removal)

Value:

14.1

Sampling time:

28 d

BOD5 / COD results

Results with reference substance:

78% after 28 days

Any other information on results incl. tables

Biodegradation rate based on oxygen consumption:

Day	Biodegradation rate [%]
	Blank	Test substance	Reference substance
5	4.3	0	67
10	5.2	0	76
15	5.7	0	78
20	5.7	0	78
25	5.7	0	78
28	5.7	0	78

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed