Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 212-634-7 | CAS number: 834-12-8
Toxicity to terrestrial plants
Administrative data
Link to relevant study record(s)
Description of key information
In general, based on phytotoxicity, plant height and dry weight, monocotyledons where less sensitive to the test substance than dicotyledons. For monocotyledons, the NOECs ranged between 0.4 to 0.05 lb active ingredient/acre (lb ai/a). For dicotyledons, the NOECs ranged between 0.2 to less than 0.006 lb ai/a.
The CSR attached within this dossier indicates that the PNECsoil based on the equilibrium partitioning method is 0.00057 mg/kg and the regional PECsoil is 0.000000159 mg/kg dw. Thus, the PECsoil / PNECsoil ratio is less than 0.01. Therefore, according to both the general risk assessment scheme and the integrated testing strategy in Figures R.7.11-2 and R.7.11-3 of TGD 7c, no additional long-term toxicity testing for soil organisms needs to be done.
In accordance with column 2 of REACH Annex X, the long-term toxicity testing on plants (required in section 9.4.6) does not need to be conducted as the chemical safety assessment according to Annex I indicates no need to investigate further the effects of the substance and/or degradation products on terrestrial organisms.
TDG 7c: Guidance on information requirements and chemical safety assessment Chapter R.7c: Endpoint specific guidance (November 2012; version 1.1)
Based on seedling emergence and growth, dicotyledons were less sensitive than monocotyledons. Onion (Allium cepa) was the only plant species to exhibit a germination dose response determined to correspond to a NOEC of 2 lb ai/a. Based on seedling growth, a dose response was determined for the dicotyledons between 2.25 x 10E11 and 2478 lb ai/a, and for monocotyledons between 47.2 and 23 lb ai/a.
Key value for chemical safety assessment
Additional information
There are two short-term plant studies, a vegetative vigour test and a seedling emergence toxicity test. These tests are specific to a part of the plants life-cycle and do not cover chronic effects or effects on reproduction.
Toxicity to terrestrial plants.001 - vegetative vigor - 1989 – key: Short-term vegetative vigour test to six dicotyledons and four monocotyledons was assessed according to the methods defined by the US Environmental Protection Agency Office of Pesticide Programs (EPA OPP) 121-1 (Target Area Phytotoxicity) according to GLP. The study exposed the plants to the test substance after growth of 1-3 true leaves by overhead spraying of the test substance combined with the irrigation water for 5 non-consecutive days followed by a 21 day observation period. The plants were grown under greenhouse conditions in natural soil. Irrigation occurred at, and daily by overhead nozzles placed 1.2 meters apart for 3 hours such that 26.15 mL of water per application was applied. Treatment at concentrations of 4.0 and 8.0 lb active ingredient/acre (lb ai/a) results in total plant death by the 21 day observation period in all plant species, except soybean. Based on phytotoxicity, plant height, and dry weight the oat (Avena sativa) and ryegrass (Lolium perenne) have NOECs of 0.8 to 0.4 lb ai/a, corn (Zea mays) have NOECs of 0.8 to 0.2 lb ai/a, carrot (Daucus carota) and cabbage (Brassica oleracea var. capitata) have NOECs of 0.4 to 0.2 lb ai/a, onion (Allium cepa) have NOECs of 0.1 to 0.05 lb ai/a, soybean (Glycine max) have NOECs of 0.1 to 0.006 lb ai/a, tomato (Lycopersicon esculentum) have NOECs of 0.05 to 0.025 lb ai/a, and cucumber (Cucumis sativus) and lettuce (Lactuca sativa) have NOECs of 0.013 to less than 0.006 lb ai/a. In general, based on phytotoxicity, plant height and dry weight, monocotyledons where less sensitive to the test substance than dicotyledons.
Toxicity to terrestrial plants.002 - seed germination – 1988: Short-term seedling emergence toxicity test to six dicotyledons and four monocotyledons according to the methods defined by the US Environmental Protection Agency Office of Pesticide Programs (EPA OPP) 121-1 (Target Area Phytotoxicity) according to GLP. Seeds were placed on filter paper in the bottom of glass petri plates. A seed treatment solution was added to each petri plate. Ten seeds of each crop were added to each petri plate, followed by the addition of the test compound. Each treatment/crop combination was replicated three times. The petri plates were incubated in the dark at 25 ± 1 ºC or 18 ± 1ºC for five to seven days. After incubation, the seeds were removed from the petri plates and the radicle length was measured to the nearest millimetre. Onion (Allium cepa) was the only plant species to exhibit a germination dose response determined to correspond to an EC50 of 644 lb active ingredient/acre (lb ai/a) and a NOEC of 2 lb ai/a. Soybean (Glycine max), carrot (Daucus carota), and cucumber (Cucumis sativus) did not demonstrate a dose response for growth. For the remaining plant species, based on growth the EC50 was determined to be for tomato (Lycopersicon esculentum) 2.25 x 1011 lb ai/a, lettuce (Lactuca sativa) 1.1 x 106 lb ai/a, cabbage (Brassica oleracea var. capitata) 2478 lb ai/a, ryegrass (Lolium perenne) 47.2 lb ai/a, oat (Avena sativa) 40.9 lb ai/a, onion (Allium cepa) 26 lb ai/a, and corn (Zea mays) 23 lb ai/a. In general, based on seedling emergence and growth, dicotyledons were less sensitive than monocotyledons.
The CSR attached within this dossier indicates that the PNECsoil based on the equilibrium partitioning method is 0.00057 mg/kg and the regional PECsoil is 0.000000159 mg/kg dw. Thus, the PECsoil / PNECsoil ratio is less than 0.01. Therefore, according to both the general risk assessment scheme and the integrated testing strategy in Figures R.7.11-2 and R.7.11-3 of TGD 7c, no additional long-term toxicity testing for soil organisms needs to be done.
In accordance with column 2 of REACH Annex X, the long-term toxicity testing on plants (required in section 9.4.6) does not need to be conducted as the chemical safety assessment according to Annex I indicates no need to investigate further the effects of the substance and/or degradation products on terrestrial organisms.
TDG 7c: Guidance on information requirements and chemical safety assessment Chapter R.7c: Endpoint specific guidance (November 2012; version 1.1)
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
