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EC number: 617-606-1 | CAS number: 84656-75-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Jul 30,2010 - Mar 01, 2011
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Principles of method if other than guideline:
- None
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Reconstituted water according to ELENDT (1990):
Macro nutrients (mg/L):
CaCI2 x 7 H20 293.80
MgSO4 x 7 H20 123.30
NaHCO3 64.80
KCI 5.80
Na2SiO3 x 9H20 10.00
NaNO3 0.27
KH2PO4 0.14
K2HPO4 0.18
Trace elements (mg/L):
B 0.5000
Fe 0.2000
Mn 0.1000
Li, Rb and Sr 0.0500
Mo 0.0250
Br 0.0125
Cu and Zn 0.0063
Co and I 0.0025
Se 0.0010
V 0.0003
Macro nutrients (mg/L):
Na2EDTA x 2H20 2.50
Vitamins (µg/L):
Thiamine 75.00
B12 1.00
Biotin 0.75
After preparation, the reconstituded water was aerated for 24 hours.
- Hardness: about 14° dH (about 250 mg/L CaCO3)
- pH: 7.9 ± 0.3, after an aeration for 24 hours.
References:
ELENDT, B.-P. Selenium deficiency in Crustacea. An ultrastructural approach to antennal damage in Daphnia magna Straus.
Protoplasma 154, 25-33, 1990 - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Species: Daphnia magna (Strauss)
Breeder: known
Age: not older than 24 hours
This species is internationally accepted for this type of study. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 14 ° dH (about 250 mg/L CaCO3)
- Test temperature:
- 22°C
- pH:
- 7.8 - 8
- Dissolved oxygen:
- at least 80 %
- Nominal and measured concentrations:
- A single test material concentration of 0.1 g/L was tested in an open static system.
- Details on test conditions:
- - Preparation
The test medium (reconstituted water and test material) was freshly prepared. Therefore, the calibrated flask with test material and vehicle, reconstituted water, was treated in an ultrasonic device for 1 hour. Subsequently the preparation was aerated, and stirred with a magnetic stirrer for further 23 hours. After 24 hours the formulation was given through a nutsch filter (pore size >10 - <16 µm). The filtrate was used for the study.
-- Analysis
The analysis of a saturated aqueous solution revealed that the solubility of the test material in water is < 0.01 mg/L. Therefore, the concentrations of the solution could not be determined analytically.
- Administration
At the start of the experimental phase, 5 Daphnia magna were placed into 10 ml of reconstituted water (control group) or test medium (test material group). The Daphnia magna were not fed, and the control medium and test medium were not aerated during the test. The test vessels were labeled to assure an unmistakable identification.
-- Dose levels
A single test material concentration of 0.1 g/L was tested in an open static system.
-- Observation schedule
The mobility was determined by visual control and recorded after 24 and 48 hours.
-- pH and oxygen concentration
The pH-values and dissolved oxygen concentration (02) were measured in the control and all test material concentrations at the beginning and at the end of the experimental part.
-- Temperature
During the experimental part, the temperature was registered in a control vessel with an electronic thermometer containing a maximum and minimum memory display.
-- Archive statement
All raw data, specimens and the final report are stored in the archives. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- A nominal test concentration of 100 mg/L revealed no aquatic toxicity in this test system.
- Results with reference substance (positive control):
- The sensitivity of the test system is demonstrated periodically. The positive control was carried out with potassium dichromate.
- Validity criteria fulfilled:
- yes
- Conclusions:
- An aqueous solution of nominal 100 mg/L of the test material revealed no aquatic toxicity in the test system. The 48 hours EC50 to Daphnia magna exceeded the solubility (< 0.01 mg/L) of the test material in reconstituted water and, thus, could not be determined in this test.
- Executive summary:
The objective of this study was to determine the acute toxicity of the test material using Daphnia magna.
For this purpose, juvenile Daphnia magna were exposed to an aqueous test material preparation over 48 hours, under defined conditions. The study comprised of four test vessels per concentration containing five Daphnia magna each, i.e. 20 Daphnia per concentration (test medium group). In addition, a control group of 20 Daphnia in reconstituted water was used.
Daphnia magna were exposed to a test material concentration of nominal 100 mg/L (limit test) in an open static system.
After exposure to a saturated aqueous solution of the nominal concentration of 0.1 g/L for 48 hours to Daphnia magna the following results were obtained:
Nominal concentration Immobilization immobilized / exposed
[g/L] 24 hours 48 hours 0.0 0/20 0/20 0.1 0/20 0/20 Daphnia magna exposed to an aqueous preparation of the nominal concentration of 100 mg/L of test material were not affected.
The test material concentration in the aqueous medium was not quantified at the start and the end of this study due to the very low water solubility (< 0.01 mg/L) and the fact that no toxicity was observed up to the limit of water solubility (< 0.01 mg/L).
For the test material the following nominal EC50 values for Daphnia magna were determined:
24 h EC50 > 100 mg/L
48 h EC50 > 100 mg/L
Conclusions
An aqueous solution of nominal 100 mg/L of the test material revealed no aquatic toxicity in the test system. The 48 hours EC50 to Daphnia magna exceeded the solubility (< 0.01 mg/L) of the test material in reconstituted water and, thus, could not be determined in this test.
Reference
Description of key information
The information for this endpoint study record was obtained from an experimental study. The OECD GLP criteria were met and the methods applied are fully compliant with OECD TG 202. An aqueous solution of nominal 100 mg/L of the test material revealed no aquatic toxicity in the test system. The 48 hours EC50 to Daphnia magna exceeded the solubility (< 0.01 mg/L) of the test material in reconstituted water and, thus, could not be determined in this test.
EC50 (48h) > 100 mg/L
Key value for chemical safety assessment
Additional information
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