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EC number: 226-685-8
CAS number: 5451-76-3
According to "Ministry of environmental protection of the people's Republic of China, The Guidelines for the Testing of Chemicals-Effects on Biotic System, 2013, 204 Fish Prolonged Toxicity Test: 14-day Study", the prolonged toxicity of the test substance, 2-Butoxyethyl benzoate (DOWM2014041 0-0882), to Gobiocypris rarus was determined by a semi-static test.
The test substance was added in sterilized test water (test water autoclaved at 121°C for 20 min) and stirred (via magnetic stirrer) in the dark for 24h at 500 rpm to prepare a stock solution with a nominal concentration of 100 mg/L. The test solutions were prepared by dilution of the stock solution with test water. Based on the results of fish acute toxicity test with the same test substance, five treatment groups, a sterilized test water control group and a control group were included in the test. The nominal concentrations of the treatment groups were 0.3, 0.5, 0.9, 1.7 and 3.0 mg/L, which the time-weighed mean measured concentrations were 0.147, 0.240, 0.409, 1.41 and 2.54 mg/L, respectively. All treatment groups, sterilized test water control group and control group had 3 replicates each containing 5 fish. The frequency of the test solution renewal was 24h and the test duration was 14 days.
The mortality in the control group and sterilized test water control group were both zero at the end of the test and the dissolved oxygen concentration was greater than 60.0% of the air saturation value (ASV) throughout the test. So the test was considered to be valid.
Results from the UPLC analysis of the test solutions showed that the measured concentrations of the newly prepared (renewal) test solutions varied less than ± 20% of their geometric mean measured concentrations during the test. However, though measures were taken to prevent degradation of the test substance in the test solutions (e.g. 24-h renewals in clean vessels, stock solutions prepared in sterile water), the measured concentrations of the test substance declined and varied more than 20% over the renewal periods (old test solutions). Thus, results were expressed as the time-weighed mean measured concentrations. Under the tested conditions, the LC50 of Gobiocypris rarus exposed to the test substance for 14 days was 0.338 mg/L with 95% confidence interval (CI) of 0.287-0.416 mg/L; the no obsersed effect concentration (NOEC) was 0.147 mg/L; the threshold level of the lethal effect was 0.240 mg/L.
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