Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 947-217-5
CAS number: -
vitro skin corrosivity test of test item was performed in a
reconstructed human epidermis model. EPISKIN (SM) is designed to predict
and classify the corrosive potential of chemicals by measuring its
cytotoxic effect as reflected in the MTT
(3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay).
The corrosivity of the test item was evaluated according to the OECD No.
EPISKIN (SM) (two units) were treated with test item and incubated for 4
hours at room temperature. Exposure of test material was terminated by
rinsing with Phosphate Buffered Saline solution. The viability of each
disk was assessed by incubating the tissues for 3 hours with MTT
solution. The precipitated formazan crystals were then extracted using
acidified isopropanol and quantified spectrophotometrically.
Physiological saline (0.9% (w/v) NaCl solution) and glacial acetic acid
treated epidermis were used as negative and positive controls,
respectively (two units / control). Two additional disks were used to
provide an estimate of colour contribution (NSC living) from the test
item. The possible MTT interaction potential of the test item was
examined using two additional test item treated and two negative control
treated killed epidermis units. Furthermore, to avoid a possible double
correction for colour interference, a third set of controls for
non-specific colour in killed tissues (NSCkilled), two
additional disks were used. For each treated tissue viability was
expressed as a % relative to the negative control. If the mean relative
viability after 4 hours of exposure is below 35% of the negative
control, the test item is considered to be corrosive to skin.
exposure to test item, the mean cell viability was 107.7 % compared to
the negative control (after adjustment for non-specific MTT reduction).
This is above the threshold of 35%, therefore the test item was
considered as being non-corrosive. The experiment met the validity
criteria, therefore the study was considered to be valid.
from the in vitro EPISKIN (SM) indicate that the test item is
non-corrosive to skin.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again