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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
2004
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
yes
Details on sampling:
Triplicate samples (3 x 2 x ~5 mL) were taken at the beginning of the test from the test solution, then 2 x ~5 mL sample was taken from each replicate at the end of the experiment. Samples from the control were taken at the start and end of the experiment (1 x 2 x ~5 mL).
Test item solutions were sampled after the preparation and were stored in freezer until measurement.
Vehicle:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Species and strain: Daphnia magna (Straus)
- Age at study initiation (mean and range, SD): Less than 24 h old at the beginning of the test.
- Source: Hungarian University of Agriculture and Life Sciences, Department of Aquaculture 2100 Gödöllő, Páter Károly u. 1. – Hungary
- Feeding during test: no

ACCLIMATION
To avoid the necessity of adaptation prior to the test, the water used in the test was similar to the culture conditions (water temperature, background colour, light intensities).
- Type and amount of food: The Daphnia were fed with concentrated algal suspension of Raphidocelis subcapitata during the holding and were not fed during the test.
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Hardness:
231 mg/L (as CaCO3)
Test temperature:
21.2 - 21.9°C
pH:
7.39 – 7.75
Dissolved oxygen:
8.3 – 8.9 mg/L
Nominal and measured concentrations:
nominal: 100 mg/L
measured geometric mean: 102.3 mg/L

Details on test conditions:

TEST SYSTEM
- Volume of solution: 40 ml/flask
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water (ISO medium, according to OECD 202)

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16-hour light and 8-hour dark cycle


RANGE-FINDING STUDY
- Test concentrations: 0.1, 1, 10, 100 mg/l
- Results used to determine the conditions for the definitive study: yes, no significant immobilisation => limit test
Reference substance (positive control):
yes
Remarks:
Potassium dichromate is tested at least twice a year to demonstrate satisfactory test conditions. The date of the last study (Study Code: 22/137-023DA) with reference item Potassium dichromate (batch no.: A0430787) is: 24 - 25 June 2022.
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 115 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Results with reference substance (positive control):
24h EC50: 0.63 mg/L, (95 % confidence limits: 0.52 – 0.76 mg/L).
This result falls within the acceptable range (0.6 mg/L to 2.1 mg/L) hence this reference study shows that the acute immobilization test on Daphnia magna is valid in this laboratory.

No abnormal behaviour or appearance of test animals was detected.

Validity criteria fulfilled:
yes
Conclusions:
There were no immobilized animals in the test item treated group and in the control group.
Under the conditions of this Daphnia magna acute immobilisation study the observed 48h EC50 for N-Glycyl-L-tyrosine dihydrate is > 115 mg/L corresponding to a 48h EC50 of > 100 mg/L for the active ingredient N-Glycyl-L-tyrosine.

Description of key information

Short-term toxicity to aquatic invertebrates: EC50 (48 h): > 100 mg/L (nominal, OECD202)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Dose descriptor:
EC50
Effect concentration:
> 100 mg/L

Additional information