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EC number: 211-525-1 | CAS number: 658-79-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: other routes
Administrative data
- Endpoint:
- acute toxicity: other routes
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1987-09-14 - 1987-10-28
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 987
- Report date:
- 1988
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- - Principle of test: Intravenous infusion of either 20 or 25 mmol/kg bw at 250 ml/kg bw N-glycyl-L-tyrosine via the lateral tail vein for a single 8h period. Subsequently the animals were observed for 14 days.
- Short description of test conditions: Animals were housed singly and fed ad libitum with free access to water except overnight before the treatment. Dose administration was effected using a 27G Microflex infusion set (Vygon) connected to a 2 way stopcock, syringe and calibrated peristaltic infusion pump (Natson Marlow, England). The pH of the administered solution was 5.6. During the 8 h infusion period and for +2 h post infusion the animals were monitored continuously and observations recorded at 30 min intervals, in the subsequent 14 days the animals were observed at least twice daily, body weight was recorded daily. On day 15 after administration the animals were sacrificed and necropsies were performed. Additionally the organ weights of brain, liver, kidneys, heart, lungs, spleen and testes/ovaries were determined.
- Parameters analysed / observed: body weight, clinical signs, mortality, gross necropsy, organ weights. - GLP compliance:
- yes
Test material
- Reference substance name:
- N-glycyl-L-tyrosine
- EC Number:
- 211-525-1
- EC Name:
- N-glycyl-L-tyrosine
- Cas Number:
- 658-79-7
- Molecular formula:
- C11H14N2O4
- IUPAC Name:
- glycyl-L-tyrosine
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: Males: 94-116 g; females: 95-189 g
- Fasting period before study: overnight
- Housing: singly in solid bottom polypropylene cages (42 x 27 x 20 cm) with stainless steel mesh tops
- Diet (e.g. ad libitum): ad libitum; Rat and Mouse (Modified) No.1 Diet SQC Expanded, supplied by Special Diet Services Limited, Stepfield, Witham, Essex, England
- Water (e.g. ad libitum): ad libitum; Domestic mains quality drinking water
- Acclimation period: 17 days prior to dosing
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20°C ± 2°C
- Humidity (%): 55% ± 10%
- Air changes (per hr): 15-20 air changes per hour
- Photoperiod (hrs dark / hrs light): 12h light-dark cycle
Administration / exposure
- Route of administration:
- intravenous
- Vehicle:
- other: electrolyte solution consisting of 50 mmol/L sodium and 10 mmol/L potassium (sodium chloride and potassium chloride respectively)
- Details on exposure:
- slow 8h infusion via the tail vein
- Doses:
- 20 and 25 mmol/kg bw
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: daily
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, organ weights, mortality
Results and discussion
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 5 956 mg/kg bw
- Based on:
- act. ingr.
- Remarks on result:
- other: Exposure time: 8 hour(s)
- Mortality:
- not observed
- Clinical signs:
- 1/5 animals for each sex showed epistaxis
- Body weight:
- Body weight profiles were considered satisfactory with all animals showing a weight gain over the 14 day post infusion observation period.
- Gross pathology:
- Gross abnormalities observed at necropsy included areas of red/purple colouration on the kidneys and mottled appearance of the liver or kidneys. Since these findings were noted in occasional animals from the treatment groups, it is considered that they were incidental and independent of dosage level.
Applicant's summary and conclusion
- Conclusions:
- N-glycyl-L-tyrosine was intravenously administered via the tail vein within 8 hours to female and male Sprague-Dawley rats. The animals were observed for 14 days after administration and clinical signs, body weight, mortality, gross necropsy and organ weight were determined. No treatment related changes occurred during the 14 days observation period. Thus, the LD50 is considered to be > 5956 mg/kg bw.
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