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EC number: 211-525-1 | CAS number: 658-79-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: control and limit concentration
- Sample storage conditions before analysis: samples from the start of the study were stored in freezer until measurement - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: a concentration of 100 mg anhydrous form of test item/L (nominal) was prepared with direct addition of the test item, mixed into the algal growth medium (OECD Medium)
- Controls: OECD Medium
- Test concentration separation factor: limit test
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): no - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Raphidocelis subcapitata
- Strain: 61.81 SAG
- Source (laboratory, culture collection): Collection of Algal Cultures, Inst. Plant Physiology, University of Göttingen, GERMANY
ACCLIMATION
- Culturing media and conditions (same as test or not): same conditions as the test
- Any deformed or abnormal cells observed: no - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Test temperature:
- 22.6-23.0°C measured in the flask
22.0-23.6°C measured within the climate chamber - pH:
- 7.29–8.76
- Nominal and measured concentrations:
- 115 mg test item /L (nominal) corresponding to 100 mg N-Glycyl-L-tyrosine/L (calculated as anhydrous form of test item)
As the measured concentration deviated not more than 20 per cent from the nominal during the test, biological results are based on the nominal test item concentration. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL Erlenmeyer flasks
- Type (delete if not applicable): open
- Aeration: Erlenmeyer flasks were continuously shaken ; flasks were covered with air-permeable stoppers
- Initial cells density: approximately 10000 algal cells per mL test medium
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no
- Photoperiod: flasks were continuously illuminated
- Light intensity and quality: ~ 8162 lux (equivalent to ~110 µE/m2/s)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: microscopic method
TEST CONCENTRATIONS
- Spacing factor for test concentrations: limit test
- Range finding study: yes
- Test concentrations of Range finding study: 0.1, 1, 10, 100 mg N-Glycyl-L-tyrosine/L
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- For the evaluation of the quality of the algae and validation of the experimental conditions, potassium dichromate is tested once a year to demonstrate satisfactory test conditions.
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 115 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- yield
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 115 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 115 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- yield
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 115 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- yield
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- yield
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Results with reference substance (positive control):
- ErC50 1.01 mg/L
95 % conf. limits 0.81 – 1.26 mg/L - Reported statistics and error estimates:
- For determination of the NOEC and the ECx the approaches recommended in the OECD guideline 201 were used. An effect was considered to be significant if statistical analysis of the data obtained for the test concentrations compared with those obtained in the negative control revealed significant inhibition of growth rate or inhibition of yield (William Multiple Sequential t-test, α=0.05, one-sided, smaller). This statistical comparison was preceded by a check on normal distribution of the data (Shapiro-Wilk’s Test) and a test for homogeneity of the variances (Levene’s test).
ToxRat Professional v 3.3.0 (ToxRat Solutions® GmbH, Germany) was used to perform the data analysis. - Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the present study with Raphidocelis subcapitata, the test item had no toxic effect on growth rate and yield of this freshwater algae species at the Limit concentration (100 mg N-Glycyl-L-tyrosine/L).
Reference
number of cells - cell concentrations (x 104 cell/mL) during the experiment
Test group | Replicate | 0h | 24h | 48h | 72h |
Control | 1 | 1 | 5 | 18 | 68 |
2 | 1 | 4 | 16 | 72 | |
3 | 1 | 3 | 18 | 67 | |
4 | 1 | 3 | 20 | 73 | |
5 | 1 | 4 | 16 | 65 | |
6 | 1 | 4 | 20 | 73 | |
Mean | 1 | 3.7 | 18 | 69.7 | |
SD | 0 | 0.8 | 1.8 | 3.4 | |
100 mg N-Glycyl-L-tyrosine /L (nominal) | 1 | 1 | 4 | 21 | 73 |
2 | 1 | 5 | 17 | 65 | |
3 | 1 | 4 | 16 | 72 | |
4 | 1 | 4 | 17 | 70 | |
5 | 1 | 4 | 20 | 66 | |
6 | 1 | 3 | 19 | 71 | |
Mean | 1 | 4 | 18.3 | 69.5 | |
SD | 0 | 0.6 | 2 | 3.3 |
Description of key information
ErC50 (72 h): >100 mg/L (nominal, OECD201)
NOErC (72h): >/= 100 mg/L (nominal, OECD201)
Key value for chemical safety assessment
- EC10 or NOEC for freshwater algae:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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