Resinoid of Boswellia Carterii (Burseraceae) obtained from exudate by organic solvents extraction.

Boiling point

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

boiling point

Type of information:

experimental study

Adequacy of study:

key study

Study period:

05-12 February 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

The study was conducted according to an internationally recognised method, and under GLP. The test substance is adequately characterised. Therefore full validation applies.

Qualifier:

according to guideline

Guideline:

OECD Guideline 103 (Boiling Point)

Version / remarks:

1995

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.2 (Boiling Temperature)

Version / remarks:

2008

Deviations:

no

Principles of method if other than guideline:

Not applicable

GLP compliance:

yes (incl. QA statement)

Remarks:

Inspected on 13 and 14 October 2014 / Signed on 8 April 2015

Type of method:

method according to Siwoloboff

Key result

Boiling pt.:

167.4 °C

Atm. press.:

>= 99 477 - <= 99 721 Pa

Decomposition:

yes

Decomp. temp.:

>= 140 °C

Pre-test:

The test tube with test item was set into the aluminium block at 24.2 °C. The aluminium block was heated with gas burner.

The following observations were noted:

Observations	Temperatures
At the bottom slightly shrivelled together	Since 50 °C
Completely melted to a yellow clear sticky mass	82.3 °C
Lots of fine foam / bubbles (on the bottom clear and on top foam)	Since 130 °C
Foam rose up	Since 150 °C
Bubble chain with lots of foam, gas burner switched off	175 °C
Bubble chain broke	174.6 °C

Main test:

Observations	Temperatures
	First Determination atmospheric pressure 99477 Pa	Second Determination atmospheric pressure 99721 Pa
Test start: Test item melted immediately to a clear yellow sticky mass with lots of fine foam. The foam vanish slowly and rose up, the sample became a liquid.	140.0 °C	136.0 °C
Some bubbles and sample getting slightly darker	142 °C	140 °C
No foam anymore	145 °C
Boiling chain, gas burner switched off	173.7 °C	178.1 °C
Boiling chain broke	168.6 °C	166.2 °C

After cooling down the test item appeared as a yellow-brown clear solid.

No calculation following Sydney-Young was carried out as decomposition (change of colour) is considered.

Results of boiling point:

	Boiling point under decomposition	Pressure in Pa
First determination	168.6 °C (441.8 K)	99477 Pa
Second determination	166.2 °C (439.4 K)	99721 Pa
Mean	167.4 °C (440.6 K)

Mean ± standard deviation (without correction following Sydney Young)= 167.4 ± 1.7 °C (440.6 ± 1.7 K)

Conclusions:

The determination of the boiling point of test item was performed following the Siwoloboff Method.
First signs of possibly decomposition indicated by colour change were observed since 140°C (lowest value).
Therefore the boiling point of test item should be stated as: 167.4 ± 1.7 °C (440.6 ± 1.7 K) under decomposition of the test item.
These values are the mean of two independent determinations without Sydney-Young correction.

Executive summary:

The study was performed in order to determine the boiling point of test item according to OECD 103, resp. EU A.2, guideline, using the Siwoloboff method.

The boiling point of test item should be stated as: 167.4 ± 1.7 °C (440.6 ± 1.7 K) under decomposition of the test item.

Reference 2

Endpoint:

boiling point

Type of information:

experimental study

Adequacy of study:

key study

Study period:

6-14 February 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

The study was conducted according to an internationally recognised method, and under GLP. The test substance is adequately characterised. Therefore full validation applies.

Qualifier:

according to guideline

Guideline:

OECD Guideline 103 (Boiling Point)

Version / remarks:

1995

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.2 (Boiling Temperature)

Version / remarks:

2008

Deviations:

no

Principles of method if other than guideline:

Not applicable

GLP compliance:

yes (incl. QA statement)

Remarks:

Inspected on 13 and 14 October 2014/ Signed on 8 April 2015

Type of method:

method according to Siwoloboff

Key result

Boiling pt.:

179.2 °C

Atm. press.:

>= 99 350 - <= 99 910 Pa

Decomposition:

yes

Decomp. temp.:

>= 120 °C

Pre-test:

The test tube with test item was set into the aluminium block at 18.1 °C. The aluminium block was heated with gas burner.

The following observations were noted:

Observations	Temperatures
Shrivelled together	44.1 °C
Completely melted to a yellow clear sticky mass	65 °C
On surface small bubbles with colourless condensate, lots of fine foam. The foam vanishes slowly.	Since 100 °C
Individual bubble	Since 120 °C
No foam anymore	Since 125 °C
Getting slightly turbid	Since 135 °C
Bubble chain, gas burner switched off	195 °C
No bubbles anymore	179.1 °C

After cooling down the test item appeared as a yellow-brown clear solid.

Main test:

Observations and measured values are presented in the following table.

Observations	Temperatures
	First Determination atmospheric pressure 99350 Pa	Second Determination atmospheric pressure 99910 Pa
Test start: Test item melted to a clear yellow viscous liquid with lots of fine foam.The foam vanishes slowly. Colourless condensate visible.	120.0 °C
Some bubbles in large interval.Sample slightly turbid.	Since 126 °C
No foam anymore.	Since 126 °C	Since 129 °C
Boiling chain, gas burner switched off	202.7 °C	198.5 °C
Boiling chain broke	179.7 °C	178.7 °C

After cooling down the test item appeared as a yellow-brown clear solid.

No calculation following Sydney-Young was carried out as a sign of possibly decomposition (colourless condensate) is considered.

Results:

	Boiling point under decomposition	Pressure in Pa
First determination	179.7 °C (452.9 K)	99350 Pa
Second determination	178.7 °C (451.9 K)	99910 Pa
Mean	179.2 °C (452.4 K)

Mean ± standard deviation (without correction following Sydney Young)= 179.2 ± 0.7 °C (452.4 ± 0.7 K).

Conclusions:

The determination of the boiling point of test item was performed following the Siwoloboff Method.
First signs of possibly decomposition indicated by a colourless condensate was observed since 120°C.
Therefore the boiling point of test item should be stated as: 179.2 ± 0.7 °C (452.4 ± 0.7 K) under decomposition of the test item.
This value is the mean of two independent determinations without Sydney-Young correction.
No observations were made which might cause doubts on the validity of the study outcome. Therefore, the result of the study is considered valid.

Executive summary:

The study was performed in order to determine the boiling point of test item according to OECD 103, resp. EU A.2, guideline, using the Siwoloboff method.

The boiling point of test item should be stated as: 179.2 ± 0.7 °C (452.4 ± 0.7 K) under decomposition of the test item.

Description of key information

The boiling point under decomposition of test item was determined to be 167.4°C or 179.2°C depending the composition tested.

Additional information

Two fully reliable experimental studies, conducted according to OECD 103/ EU A2 guideline are available.

Both are considered as a key study, and the result is retained as key data.