Diisobutyl adipate

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1994

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: OECD Test Guideline 202

Version / remarks:

(1984)

Deviations:

no

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Dibutyl adipate

EC Number:

203-350-4

EC Name:

Dibutyl adipate

Cas Number:

105-99-7

Molecular formula:

C14H26O4

IUPAC Name:

dibutyl adipate

Test material form:

liquid

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

Stock solution was prepared with DMSO:HCO = 9:1 (100 mg/l). Controls with and without this vehicle were taken for test.

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Test conditions

Nominal and measured concentrations:

40 daphnids (4 replicates; 10 organisms per replicate) were exposed to each of 5 nominal concentrations (0.18-18 mg/l).

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

21 d

Dose descriptor:

LC50

Effect conc.:

ca. 4.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

immobilisation

Effect concentrations 2

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

ca. 9.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

immobilisation

Effect concentrations 3

Key result

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

ca. 4.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Effect concentrations 4

Key result

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

ca. 5.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Effect concentrations 5

Key result

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

ca. 18 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Any other information on results incl. tables

Type of test: static [ ]; semi-static [X]; flow-through [ ]; other [ ];

open-system [X]; closed-system [ ]

Species: Daphnia magna

End-point: Mortality [ ]; Reproduction rate [X]; Other [X]

Exposure period: 21 day

Results:

Immobility: LC50 (24 h) =

LC50 (48 h) = 9.3 mg/l

LC50 (96 h) =

LC50 ( 7 d) =

LC50 (14 d) =

LC50 (21 d) = 4.3 mg/l (95% confidence level: 3.4-5.4 mg/l)

NOEC =

LOEC =

Reproduction: EC50 (14 d)= l

EC50 (21 d) = 8.9 mg/l(95% confidence level:4.5-28 mg/l)

NOEC = 5.6 mg/l (p < 0.05)

LOEC = 18 mg/l (p < 0.05)

Analytical monitoring: Yes [ ] No [X] ? [ ]

Method: OECD Test Guideline 202 (1984)

GLP: Yes [ ] No [X] ? [ ]

Test substance: Dibutyl adipate, purity = 99 %

Remarks: 40 daphnids (4 replicates; 10 organisms per replicate) were exposed

to each of 5 nominal concentrations (0.18-18 mg/l). Stock solution was prepared with DMSO:HCO = 9:1 (100 mg/l).

Controls with and without this vehicle were taken for test.

Reference: EA, Japan (1994)