Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.

REACH

Reaction mass of 1-Propanaminium, 3-[[2-cyano-3-[4-(diethylamino)phenyl]-1-oxo-2-propen-1-yl]oxy]-N-[2-[[2-cyano-3-[4-(diethylamino)phenyl]-1-oxo-2-propen-1-yl]oxy]ethyl]-N,N-dimethyl- & chloride

EC number: 947-402-0 | CAS number: -

Life Cycle description
Uses advised against

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed according to the OECD guideline.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

This study was designed to assess the toxic effects of the test compound on the Zebra fish (Danio rerio). Test was conducted in compliance with the OECD guideline 203.

GLP compliance:

not specified

Specific details on test material used for the study:

Name: Reaction mass of 1-Propanaminium, 3-[[2-cyano-3-[4-(diethylamino)phenyl]-1-oxo-2-propen-1-yl]oxy]-N-[2-[[2-cyano-3-[4-(diethylamino)phenyl]-1-oxo-2-propen-1-yl]oxy]ethyl]-N,N-dimethyl- & chloride Mol. formula: C35H46ClN5O4Molecular Weight: 636.232 g/moleInChI:1S/C35H46N5O4.ClH/c1-7-38(8-2)32-16-12-28(13-17-32)24-30(26-36)34(41)43-22-11-20-40(5,6)21-23-44-35(42)31(27-37)25-29-14-18-33(19-15-29)39(9-3)10-4;/h12-19,24-25H,7-11,20-23H2,1-6H3;1H/q+1;/p-1/b30-24+,31-25+;Smiles:C(CCOC(\C(=C\c1ccc(cc1)N(CC)CC)C#N)=O)[N+](C)(C)CCOC(\C(=C\c1ccc(cc1)N(CC)CC)C#N)=O.[ClH-]Substance type: Organic

Analytical monitoring:

not specified

Vehicle:

yes

Details on test solutions:

The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 100 mg of the test substance in 100 ml of potable water (passed through reverse osmosis system) with continuous one hr stirring, stock was analytically detected, solubility found 1015.78 mg/L.On the basis of solubility value following test concentrations prepared 1.25mg/L,2.5mg/L,5mg/L,10mg/L,20mg/L ,respectively.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM- Common name: Zebra fish(Danio rerio)- Source: Anytime Pet, Jariphataka, Nagpur- Length at study initiation (length definition, mean, range and SD): average 1.29 cm- Weight at study initiation (mean and range, SD): 0.049g (average)ACCLIMATION- Acclimation period: 7 days- Acclimation conditions (same as test or not): Same as test- Feeding frequency during acclimation: Daily once- Health during acclimation (any mortality observed): No mortality were observedOther conditions during acclimation: - Photoperiod 16 hours light and 8 hours dark- Temperature range: 24.1 °C- pH range 7.1- DO range 7.0 mg/l- Aeration Continous

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Post exposure observation period:

24, 48, 72, 96 hrs

Hardness:

155.5 (mg of CaCO3)

Test temperature:

23 °C

pH:

7.2

Dissolved oxygen:

7 mg/l

Nominal and measured concentrations:

1.25mg/L, 2.5mg/L, 5mg/L, 10mg/L and 20mg/L

Details on test conditions:

TEST SYSTEM - Test vessel: Plastic aquaria - Material, size, headspace, fill volume: Plastic aquaria having the water capacity of 5 liters filled with 2 liters of water with 3 liter of headspace. - Aeration: Aeration in test vessels was provided 1 day before the start of experiment - No. of organisms per vessel: 8 - No. of vessels per concentration (replicates): 2 OTHER TEST CONDITIONS - Adjustment of pH: No - Photoperiod: 16 hours light and 8 hours dark EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Observations of (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 24 hours, 48 hours, 72 hours and 96 hours of the start of the experiment.

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: No effect were observed

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Toxic to fish

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

20 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Other details not known

Sublethal observations / clinical signs:

Fig:Mortality and symptoms

Nominal Concentration (mg/l)	Number of fish		Mortality (cumulated) after
			3hr			6hr		24hr			48hr		72hr			96hr
			V1	V2		V1	V2	V1	V2		V1	V2	V1	V2		V1	V2
Control	4	4	-	-		-	-	-	-		-	-	-	-		-	-
6.25	4	4	-		-	-	-	-		-	-	-	-		-	-		-
12.5	4	4	-		-	-	-	-		-	-	-	-		-	-		-
25	4	4	-		-	-	-	-		-	-	-	-		-	-		-
50	4	4	-		-	-	-	-		-	-	-	1		1	2		2
100	4	4	-		-	-	-	-		-	2	2	4		4	4		4

Fig: Visible Symptoms:

Nominal

Concentration

(mg/l)

Number of

fish

symptom (cumulated) after

3hr

6hr

24hr

48hr

72hr

96hr

Control

NS4

6.25

NS4

12.5

NS4

100

NS4

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

8.2. pH and oxygen content:

		Number of	pH readings after
Nominal	fish		24h		48h		72h		96h
Concentration			V1	V2	V1	V2	V1	V2	V1	V2
(mg/l)
Control	4	4	7.3	7.3	7.3	7.3	7.4	7.4	7.5	7.5
1.25	4	4	7.4	7.4	7.5	7.5	7.6	7.6	7.7	7.7
2.5	4	4	7.4	7.4	7.5	7.5	7.6	7.6	7.7	7.7
5	4	4	7.5	7.5	7.6	7.6	7.7	7.7	7.8	7.8
10	4	4	7.6	7.6	7.7	7.7	7.8	7.8	7.9	7.9
20	4	4	7.7	7.7	7.8	7.8	-	-	-	-

Nominal	Number of		Oxygen content (mg/L) after
Concentrationfish			24h		48h		72h		96h
(mg/l)			V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	7.0	7.0	6.9	6.9	6.8	6.8	6.7	6.7
1.25	4	4	6.9	6.9	6.8	6.8	6.7	6.7	6.6	6.6
2.5	4	4	6.9	6.9	6.8	6.8	6.7	6.7	6.6	6.6
5	4	4	6.9	6.9	6.8	6.8	6.7	6.7	6.6	6.6
10	4	4	6.9	6.9	6.7	6.7	6.6	6.6	6.5	6.5
20	4	4	6.9	6.9	6.7	6.7	-	-	-	-
8.3. Test temperature
Nominal	Number of		Temperature (°C) after
Concentrationfish			24h		48h		72h		96h
(mg/l)			V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	23.2	23.2	23.2	23.2	23.3	23.3	23.3	23.3
1.25	4	4	23.2	23.2	23.2	23.2	23.3	23.3	23.3	23.3
2.5	4	4	23.2	23.2	23.2	23.2	23.3	23.3	23.3	23.3
5	4	4	23.2	23.2	23.2	23.2	23.3	23.3	23.3	23.3
10	4	4	23.2	23.2	23.2	23.2	23.3	23.3	23.3	23.3
20	4	4	23.2 23.2		23.2	23.2	-	-	-	-

Validity criteria fulfilled:

yes

Conclusions:

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for Reaction mass of 1-Propanaminium, 3-[[2-cyano-3-[4-(diethylamino)phenyl]-1-oxo-2-propen-1-yl]oxy]-N-[2-[[2-cyano-3-[4-(diethylamino)phenyl]-1-oxo-2-propen-1-yl]oxy]ethyl]-N,N-dimethyl- & chloride on Zebra fish (Danio rerio) was determined to be 10 mg/L by observing the mortality. Thus based on LC50 it can be concluded that the chemical was toxic.

Executive summary:

This study was designed to assess the toxic effects of the test reaction mass of 1-Propanaminium, 3-[[2-cyano-3-[4-(diethylamino)phenyl]-1-oxo-2-propen-1-yl]oxy]-N-[2-[[2-cyano-3-[4-(diethylamino)phenyl]-1-oxo-2-propen-1-yl]oxy]ethyl]-N,N-dimethyl- & chloride on the Zebra fish (Danio rerio). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 100 mg of the test substance in 100 ml of potable water (passed through reverse osmosis system) with continuous one hr stirring, stock was analytically detected, solubility found 1015.78 mg/L. On the basis of solubility value following test concentrations prepared 1.25mg/L, 2.5mg/L, 5mg/L, 10mg/L, 20mg/L ,respectively. Study conducted under the static system for 96 hrs. Test animal was collected from Anytime Pet, Jariphataka, Nagpur. 8 fishes exposed to the test concentration. Aeration in test vessels was provided 1 day before the start of experiment. After the exposure of chemical (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 24 hours, 48 hours, 72 hours and 96 hours of the start of the experiment. Based on nominal concentrations, experimental median lethal concentrations [LC-50 (96 h)] for Reaction mass of 1-Propanaminium, 3-[[2-cyano-3-[4-(diethylamino)phenyl]-1-oxo-2-propen-1-yl]oxy]-N-[2-[[2-cyano-3-[4-(diethylamino)phenyl]-1-oxo-2-propen-1-yl]oxy]ethyl]-N,N-dimethyl- & chloride on Zebra fish (Danio rerio) was determined to be 10 mg/L by observing the mortality. Thus based on LC50 it can be concluded that the chemical was toxic and classified as aquatic chronic 2 as per the CLP criteria.

Description of key information

Based on nominal concentrations, experimental median lethal concentrations [LC-50 (96 h)] for Reaction mass of 1-Propanaminium, 3-[[2-cyano-3-[4-(diethylamino)phenyl]-1-oxo-2-propen-1-yl]oxy]-N-[2-[[2-cyano-3-[4-(diethylamino)phenyl]-1-oxo-2-propen-1-yl]oxy]ethyl]-N,N-dimethyl- & chloride on Zebra fish (Danio rerio) was determined to be 10 mg/L by observing the mortality. Thus based on LC50 it can be concluded that the chemical was toxic and classified as aquatic chronic 2 as per the CLP criteria.

Key value for chemical safety assessment

Fresh water fish

Effect concentration:: 10 mg/L

Additional information

This study was designed to assess the toxic effects of the test compound reaction mass of 1-Propanaminium, 3-[[2-cyano-3-[4-(diethylamino)phenyl]-1-oxo-2-propen-1-yl]oxy]-N-[2-[[2-cyano-3-[4-(diethylamino)phenyl]-1-oxo-2-propen-1-yl]oxy]ethyl]-N,N-dimethyl- & chloride on the Zebra fish (Danio rerio). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 100 mg of the test substance in 100 ml of potable water (passed through reverse osmosis system) with continuous one hr stirring, stock was analytically detected, solubility found 1015.78 mg/L. On the basis of solubility value following test concentrations prepared 1.25mg/L, 2.5mg/L, 5mg/L, 10mg/L, 20mg/L ,respectively. Study conducted under the static system for 96 hrs. Test animal was collected from Anytime Pet, Jariphataka, Nagpur. 8 fishes exposed to the test concentration. Aeration in test vessels was provided 1 day before the start of experiment. After the exposure of chemical (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 24 hours, 48 hours, 72 hours and 96 hours of the start of the experiment. Based on nominal concentrations, experimental median lethal concentrations [LC-50 (96 h)] for Reaction mass of 1-Propanaminium, 3-[[2-cyano-3-[4-(diethylamino)phenyl]-1-oxo-2-propen-1-yl]oxy]-N-[2-[[2-cyano-3-[4-(diethylamino)phenyl]-1-oxo-2-propen-1-yl]oxy]ethyl]-N,N-dimethyl- & chloride on Zebra fish (Danio rerio) was determined to be 10 mg/L by observing the mortality. Thus based on LC50 it can be concluded that the chemical was toxic and classified as aquatic chronic 2 as per the CLP criteria.

Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.