Reaction mass of 1-Propanaminium, 3-[[2-cyano-3-[4-(diethylamino)phenyl]-1-oxo-2-propen-1-yl]oxy]-N-[2-[[2-cyano-3-[4-(diethylamino)phenyl]-1-oxo-2-propen-1-yl]oxy]ethyl]-N,N-dimethyl- & chloride

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

Experimental data of read across chemical

Justification for type of information:

Data for the target chemical is summarized based on the structurally and functionally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: As mention below

Principles of method if other than guideline:

WoE report is based on short term toxicity studies on microorganisms as - WoE-2 and WoE-3

GLP compliance:

not specified

Specific details on test material used for the study:

Name: Reaction mass of 1-​Propanaminium, 3-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​-​N-​[2-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​ethyl]​-​N,​N-​dimethyl-​ & chlorideMol. formula: C35H46ClN5O4Molecular Weight: 636.232 g/moleInChI:1S/C35H46N5O4.ClH/c1-7-38(8-2)32-16-12-28(13-17-32)24-30(26-36)34(41)43-22-11-20-40(5,6)21-23-44-35(42)31(27-37)25-29-14-18-33(19-15-29)39(9-3)10-4;/h12-19,24-25H,7-11,20-23H2,1-6H3;1H/q+1;/p-1/b30-24+,31-25+;Smiles:C(CCOC(\C(=C\c1ccc(cc1)N(CC)CC)C#N)=O)[N+](C)(C)CCOC(\C(=C\c1ccc(cc1)N(CC)CC)C#N)=O.[ClH-]Substance type: Organic

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

activated sludge

Details on inoculum:

Activated sludge was obtained from a sewage works treating predominantly domestic sewage. The activated sludge may be obtained from a sewage works treating predominantly industrial waste water. The sludge was centrifuged and washed with tap water. This procedure was repeated three times. A small amount of the centrifuged wet sludge was weighed and dried. After drying the ratio R of the weight of the dry solid to that of the wet centrifuged sludge was known. From this ratio R the amount of wet sludge can be calculated which has to be suspended in tap water in order to obtain an activated sludge with a mixed liquor suspended solids level of 4 g dry solids/l. 50 ml OECD concentrate (2.2) is added to each liter of the activated sludge prepared as described above; this was then aerated overnight in a room at 20°C + 2°C, and kept aerated for use during the day.

Test type:

static

Water media type:

freshwater

Total exposure duration:

3 h

Test temperature:

20 ± 2°C

pH:

7-8

Dissolved oxygen:

<2.5 mg/l

Nominal and measured concentrations:

Nominal 100 mg/l

Details on test conditions:

- Test vessel: ACTIVATED SLUDGE RESPIRATION RATE APPARATUS- Material, size, headspace, fill volume: 500 ml flask- Aeration: Yes- Type of flow-through: "Pasteur" pipette- No. of vessels per concentration (replicates): 2- No. of vessels per control (replicates): 2

Reference substance (positive control):

not specified

Key result

Duration:

3 h

Dose descriptor:

IC50

Effect conc.:

>= 10 - <= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: Effect on the respiration rate of microorganisms

Remarks on result:

other: For both studies

Validity criteria fulfilled:

not specified

Conclusions:

1. After the exposure of test chemical for 3 hrs, inhibitory concentration at which 50 % rate of respiration affects was measured. Based on the inhibition of respiration rate IC50 was observed in the range of 10-100 mg/l. Thus based on IC50, chemical was consider as toxic and classified as aquatic chronic 3 as per the CLP criteria. 2. The IC50 value (concentration for 50% inhibition of respiration rate) for the test chemical on activated sludge (aerobic bacteria) is determined to be 10-100 mg/1 after 3 hrs of exposure.Based on the above studies, it was concluded that the Reaction mass of 1-​Propanaminium, 3-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​-​N-​[2-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​ethyl]​-​N,​N-​dimethyl-​ & chloride was toxic.

Executive summary:

Data available for the structurally and functionally similar read across chemicals study has been reviewed to determine the toxicity of the test chemical Reaction mass of 1-​Propanaminium, 3-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​-​N-​[2-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​ethyl]​-​N,​N-​dimethyl-​ & chloride on the microorganisms. The studies are as mentioned below:

The first study was conducted to investigate the toxic nature of test chemical when comes in contact with microorganisms. The test principle involves measuring the respiration rate of an activated sludge and comparing it with the respiration rate of the same activated sludge under identical conditions, but in the presence of the chemical under test. The test was carried out in activated sludge respiration rate apparatus with constant 20 ± 2°C and pH about 7-8. The test concentration used was 100 mg/l. OECD recommended synthetic sewage was used as feed, while activated sludge was obtained from a sewage works treating predominantly domestic sewage or from a sewage works treating predominantly industrial waste water. The respiration rate of an activated sludge only and with test chemical were noted down. In order to calculate the inhibitory effect of a particular chemical at 100 mg/l test concentration its respiration rate is expressed as a percentage of the mean of the two control respiration rates. After the exposure of test chemical for 3 hrs, inhibitory concentration at which 50 % rate of respiration affects was measured. Based on the inhibition of respiration rate IC50 was observed in the range of 10-100 mg/l. IC50. Thus based on IC50, chemical was consider as toxic.

Similarly in second study, screening method based on the measurement of the respiration rate of activated sludge for assessing the possible inhibitory effect of dyestuffs on aerobic waste-water bacteria. The test principle involves measuring the respiration rate of an activated sludge and comparing it with the respiration rate of the same activated sludge under identical conditions, but in the presence of the chemical under test. The test was carried out in activated sludge respiration rate apparatus with constant 20 ± 2°C and pH about 7-8. The test concentration used was 100 mg/l. OECD recommended synthetic sewage was used as feed, while activated sludge was obtained from a sewage works treating predominantly domestic sewage or from a sewage works treating predominantly industrial waste water. The respiration rate of an activated sludge and the respiration rate of activated sludge with test chemical were noted down. In order to calculate the inhibitory effect of a particular chemical at 100 mg/l test concentration its respiration rate is expressed as a percentage of the mean of the two control respiration rates. Thus, IC50 value (concentration for 50% inhibition of respiration rate) for the test chemical on activated sludge (aerobic bacteria) is determined to be10 - 100 mg/1 after 3 hrs of exposure.

Based on the above studies, it was concluded that the Reaction mass of 1-​Propanaminium, 3-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​-​N-​[2-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​ethyl]​-​N,​N-​dimethyl-​ & chloride was toxic.

Description of key information

1. After the exposure of test chemical for 3 hrs, inhibitory concentration at which 50 % rate of respiration affects was measured. Based on the inhibition of respiration rate IC50 was observed in the range of 10-100 mg/l. Thus based on IC50, chemical was consider as toxic and classified as aquatic chronic 3 as per the CLP criteria.

2. The IC50 value (concentration for 50% inhibition of respiration rate) for the test chemical on activated sludge (aerobic bacteria) is determined to be 10-100 mg/1 after 3 hrs of exposure.

Based on the above studies, it was concluded that the Reaction mass of 1-​Propanaminium, 3-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​-​N-​[2-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​ethyl]​-​N,​N-​dimethyl-​ & chloride was toxic.

Additional information

Data available for the structurally and functionally similar read across chemicals study has been reviewed to determine the toxicity of the test chemical Reaction mass of 1-​Propanaminium, 3-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​-​N-​[2-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​ethyl]​-​N,​N-​dimethyl-​ & chloride on the microorganisms. The studies are as mentioned below:

 

The first study was conducted to investigate the toxic nature of test chemical when comes in contact with microorganisms. The test principle involves measuring the respiration rate of an activated sludge and comparing it with the respiration rate of the same activated sludge under identical conditions, but in the presence of the chemical under test. The test was carried out in activated sludge respiration rate apparatus with constant 20 ± 2°C and pH about 7-8. The test concentration used was 100 mg/l. OECD recommended synthetic sewage was used as feed, while activated sludge was obtained from a sewage works treating predominantly domestic sewage or from a sewage works treating predominantly industrial waste water. The respiration rate of an activated sludge only and with test chemical were noted down. In order to calculate the inhibitory effect of a particular chemical at 100 mg/l test concentration its respiration rate is expressed as a percentage of the mean of the two control respiration rates. After the exposure of test chemical for 3 hrs, inhibitory concentration at which 50 % rate of respiration affects was measured. Based on the inhibition of respiration rate IC50 was observed in the range of 10-100 mg/l. IC50. Thus based on IC50, chemical was consider as toxic and classified as aquatic chronic 3 as per the CLP criteria.

 

Similarly in second study, screening method based on the measurement of the respiration rate of activated sludge for assessing the possible inhibitory effect of dyestuffs on aerobic waste-water bacteria. The test principle involves measuring the respiration rate of an activated sludge and comparing it with the respiration rate of the same activated sludge under identical conditions, but in the presence of the chemical under test. The test was carried out in activated sludge respiration rate apparatus with constant 20 ± 2°C and pH about 7-8. The test concentration used was 100 mg/l. OECD recommended synthetic sewage was used as feed, while activated sludge was obtained from a sewage works treating predominantly domestic sewage or from a sewage works treating predominantly industrial waste water. The respiration rate of an activated sludge and the respiration rate of activated sludge with test chemical were noted down. In order to calculate the inhibitory effect of a particular chemical at 100 mg/l test concentration its respiration rate is expressed as a percentage of the mean of the two control respiration rates. Thus, IC50 value (concentration for 50% inhibition of respiration rate) for the test chemical on activated sludge (aerobic bacteria) is determined to be10 - 100 mg/1 after 3 hrs of exposure.

 

Based on the above studies, it was concluded that the Reaction mass of 1-​Propanaminium, 3-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​-​N-​[2-​[[2-​cyano-​3-​[4-​(diethylamino)​phenyl]​-​1-​oxo-​2-​propen-​1-​yl]​oxy]​ethyl]​-​N,​N-​dimethyl-​ & chloride was toxic.