3-(Hydroxymethyl)-1-pentene

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

10.01.-25.06.2008

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

About 50 mg of the test item were dissolved in 50 mL acetonitrile to obtain a stock solution of 1 g test item/L, respectively. Appropriate amounts of this stock solution were diluted with test medium to obtain fortified samples at a level of 10 and 100 mg test item/L. Exact values were documented in the raw data.

Vehicle:

no

Details on test solutions:

Reconstituted water
CaCl2*2H2O 290.00 mg/L
MgSO4*7H2O 123.25 mg/L
NaHCO3 64.75 mg/L
KCl 5.75 mg/L

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Source: Institute of Toxicology, Merck KGaA
- Length at study initiation: 2.0 +-1.0 cm
- Weight at study initiation: mean 8.9 g (control), 8.8 g (test material group)

ACCLIMATION
- Acclimation period: 14 d
- Type and amount of food during acclimation: Tetra Min diet, Tetra GmbH, Melle, daphnia, or artemia, or frozen mosquitolarva
- Feeding frequency during acclimation: daily
- Health during acclimation (any mortality observed): mortality <5%

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

15°dH (ca. 267 mg/L CaCO3)

Test temperature:

22-25 °C

pH:

6.96 - 7.60

Dissolved oxygen:

79.1 - 93.3%

Nominal and measured concentrations:

Nominal Concentration: 100 mg/L
Measured: 90% of nominal (mean)

Details on test conditions:

TEST SYSTEM
- Test vessel: 9 L
- Aeration: 24 h before use
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water
- Ca/Mg ratio: 4.1
- Na/K ratio: 10:1

OTHER TEST CONDITIONS
- Photoperiod: 12 h Light/12 h dark

Reference substance (positive control):

not required

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 90.2 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: fish swimming near bottom of aquarium, only at 100 mg/L

Sublethal observations / clinical signs:

Mortality

	Mean analytical concentration during the study		Mortality
	[%]	[mg/L]	24 h	48 h	72 h	96 h
Control	-	-	0/10	0/10	0/10	0/10
Test item	90	90.2	0/10	0/10	0/10	0/10

Symptoms

		3h	24 h	48 h	72 h	96 h
Test item	no abnormalities detected	5	5	5	8	10
	Fish near the bottom of aquarium	5	5	5	1	0
Contol	No abnormalities detected	10	10	10	10	10

Validity criteria fulfilled:

yes

Conclusions:

The 96h-LC50 was determined to be >90.2 mg/L (mean analytical concentration).

Executive summary:

Study design

The objective of this study was to determine the acute toxicity of the test material (Ethyl-Butenol) in the zebra fish, Danio rerio. For this purpose, 10 fish (test material group) and further 10 fish (control group) were exposed over 96 hours, under defined conditions in a limit test. The fish were observed for signs of toxicity or death for 96 hours. Zebra fish of the test material group were exposed to a saturated aqueous test material solution of nominal 100 mg/L (limit test) in a closed static system.

Results

Signs of toxicity were observed in the test material group at a nominal concentration of 100.00 mg/L consisting of fish swimming near the bottom of the aquarium. They started immediately after exposure and lasted up to 72 hours. No mortality was observed throughout the test. The analytical recovery was 111 % and 70 % after 0 and 96 hours, respectively. The mean recovery over 96 hours was 90 %. As the stability of the test material in the aqueous solution could not be maintained at ≥80 %, the LC₅₀ values are based on analytical concentrations (see Addendum Analytical Phase Report). Nominal, corresponding mean analytical concentrations and mortality are given in the following table.

	Mean analytical concentration during the study		Mortality
	[%]	[mg/L]	24 h	48 h	72 h	96 h
Control	-	-	0/10	0/10	0/10	0/10
Test item	90	90.2	0/10	0/10	0/10	0/10

 

For the test material, the following LC₀, LC₅₀ and LC₁₀₀ values for zebra fish were determined:

 

24 h LC0               ≥ 90.2 mg/L

48 h LC0               ≥ 90.2 mg/L

72 h LC0               ≥ 90.2 mg/L

96 h LC0               ≥ 90.2 mg/L

 

24 h LC50            > 90.2 mg/L

48 h LC50            > 90.2 mg/L

72 h LC50            > 90.2 mg/L

96 h LC50            > 90.2 mg/L

 

24 h LC100          > 90.2 mg/L

48 h LC100          > 90.2 mg/L

72 h LC100          > 90.2 mg/L

96 h LC100          > 90.2 mg/L

 

Conclusion

An aqueous solution of the test item, of a nominal concentration of 100 mg/L, revealed no aquatic toxicity in the test system. The 96 hours LC₅₀ to zebra fish was > 90.2 mg/L and, thus, could not be determined in this test.

Description of key information

The 96h-LC50 was determined to be >90.2 mg/L.