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EC number: 202-795-1 | CAS number: 99-86-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
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- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
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- Flash point
- Auto flammability
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- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
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- Endpoint summary
- Stability
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- Environmental data
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
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- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2007
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
Data source
Reference
- Reference Type:
- publication
- Title:
- Examination of a -terpinene on Primary Eye Irritancy and Skin Sensitization
- Author:
- Park B.S.
- Year:
- 2 007
- Bibliographic source:
- Mol. Cell. Toxicol. Vol. 3(1), 68-75, 2007
- Report date:
- 2007
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
- Qualifier:
- according to guideline
- Guideline:
- other: Korean Food and Drug Administration (KFDA) guidelines
- GLP compliance:
- not specified
Test material
- Reference substance name:
- p-mentha-1,3-diene
- EC Number:
- 202-795-1
- EC Name:
- p-mentha-1,3-diene
- Cas Number:
- 99-86-5
- Molecular formula:
- C10H16
- IUPAC Name:
- 1-isopropyl-4-methylcyclohexa-1,3-diene
- Test material form:
- liquid
- Details on test material:
- Clear colourless liquid.
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: alpha terpinene was purchased from Sigma (St. Louis, MO)
FORM AS APPLIED IN THE TEST: Formulation of test repellents.
Repellent solution prepared using 0.5 g (2.0%) of alpha terpinene.
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: New Zealand white, Sejin Laboratory Co. (Seoul, South Korea)
- Age at study initiation: 12-18 weeks of age
- Weight at study initiation: male (2.8-3.3 kg) and female (2.7-3.4 kg)
- Acclimation period: yes, for 5 days before testing
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 16-21.6 °C
- Humidity (%): of 40-70%
- Air changes (per hr): not specified
- Photoperiod (hrs dark / hrs light): 12 h photoperiod
Test system
- Type of coverage:
- occlusive
- Preparation of test site:
- shaved
- Remarks:
- dorsal trunk
- Vehicle:
- other: Formulation of test repellents
- Controls:
- not specified
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): A volume of 0.5 mL repellent solution
- Concentration: 0.5 g (2.0%) of alpha terpinene in repellent solution
NEGATIVE CONTRL - yes
POSITIVE CONTROL - no - Duration of treatment / exposure:
- 4 hrours
- Observation period:
- 24 h and 72 h
- Number of animals:
- Three males and three females were tested
- Details on study design:
- TEST SITE
- Area of exposure: dorsal trunk
- % coverage: 100% After applying the test compound polyethylene wrap was secured around the trunk of the rabbit. Rabbits were placed in a restraining cage for 4 h treatment. After that time which wraps were removed.
- Type of wrap if used: 1-inch square gauze patch was placed on the skin and secured with adhesive tape.
OBSERVATION TIME POINTS - 24 h and 72 h
SCORING SYSTEM: provided
Four different skin sensitivity cohorts were examined, as follows:
(1) non-treated at intact site (NTN group)
(2) non-treated at abraded site (NTA group),
(3) 2% a -terpinene solution at intact site (2TN group),
(4) 2% a -terpinene solution at abraded site (2TA group).
Each test rabbit had 3 intact sites and 3 abraded sites.
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- erythema score
- Remarks:
- scoring based on the final results
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- > 0 - <= 2
- Max. score:
- 4
- Reversibility:
- fully reversible
- Remarks on result:
- other: no scoring provided
- Remarks:
- No acute abnormalities were observed to intact or abraded skin after application of 2% alpha terpinene in rabbits.
- Irritation parameter:
- edema score
- Remarks:
- scoring based on the final results
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- > 0 - <= 2
- Max. score:
- 4
- Reversibility:
- fully reversible
- Remarks on result:
- other: no scoring provided
- Remarks:
- No acute abnormalities were observed to intact or abraded skin after application of 2% alpha terpinene in rabbits.
Applicant's summary and conclusion
- Interpretation of results:
- other: not skin irritant
- Conclusions:
- No acute abnormalities were observed to intact or abraded skin after application of 2% alpha terpinene in rabbits.
- Executive summary:
In this study, the skin irritation potential was investigated in New Zealand white rabbits using the repellent solution containing 0.5 g (2.0%) of alpha terpinene.
The local skin reactions were read and recorded at 24 h and 72 h using the scoring system. A skin biopsy was taken at 72 h after treatment for histological studies. The skin biopsies were fixed in 10% buffered formalin solution for several days, then underwent ethanol series dehydration and embedded in paraffin. Sections were stained with hematoxylin-eosin and examined under a light microscope.
Results demonstrated, that no acute abnormalities were observed to intact or abraded skin after application of 2% alpha terpinene in rabbits.
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