2,4-difluoroaniline

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Reference 2

Reference Type:

other: Statement

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2,4-difluoroaniline

EC Number:

206-687-5

EC Name:

2,4-difluoroaniline

Cas Number:

367-25-9

Molecular formula:

C6H5F2N

IUPAC Name:

2,4-difluoroaniline

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

other: Selenastrum capricornutum

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Nominal and measured concentrations:

Control, 0.63, 1.3, 2.5, 5.0, 10, and 20 mg a.i./L (nominal)
< LOQ, 0.52, 1.0, 2.0, 4.0, 8.0, and 17 mg a.i./L (mean measured)

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

2.05 µg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: Recalculation with ToxRat Professional Vers. 2.10.05

Remarks:

95% confidence interval : 1.398 – 2.679 µg/L

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC20

Effect conc.:

4.106 µg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: Recalculation with ToxRat Professional Vers. 2.10.05

Remarks:

95% confidence interval: 3.239 – 4.918 µg/L

Effect concentrations 3

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

15.507 µg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: Recalculation with ToxRat Professional Vers. 2.10.05

Remarks:

95% confidence interval: 12.986 - 19.548

Effect concentrations 4

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

1 other: mg a.i./L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other:

Remarks:

calculation from 2002 (The SAS System for Windows", Version 8.02)

Effect concentrations 5

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

16 other: mg a.i./L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: calculation from 2002 (The SAS System for Windows", Version 8.02)

Remarks:

95% confidence interval: 13 - 18 mg a.i./L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The derived NOEC (72 h) was 1.0 mg a.i./L and the ErC50 (72 h) was 16 mg a.i./L (arithmetic mean measured;calculation from 2002 (The SAS System for Windows", Version 8.02)).
The recalculated (ToxRat Professional Vers. 2.10.05) ErC10 (72 h) is 2.050 mg/L and Er50 (72h) is 15.507 µg/L.