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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
2002
Report date:
2002
Reference Type:
other: Statement
Title:
Unnamed
Year:
2016
Report date:
2016

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
2,4-difluoroaniline
EC Number:
206-687-5
EC Name:
2,4-difluoroaniline
Cas Number:
367-25-9
Molecular formula:
C6H5F2N
IUPAC Name:
2,4-difluoroaniline

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
other: Selenastrum capricornutum

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Nominal and measured concentrations:
Control, 0.63, 1.3, 2.5, 5.0, 10, and 20 mg a.i./L (nominal)
< LOQ, 0.52, 1.0, 2.0, 4.0, 8.0, and 17 mg a.i./L (mean measured)
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
2.05 µg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: Recalculation with ToxRat Professional Vers. 2.10.05
Remarks:
95% confidence interval : 1.398 – 2.679 µg/L
Duration:
72 h
Dose descriptor:
EC20
Effect conc.:
4.106 µg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: Recalculation with ToxRat Professional Vers. 2.10.05
Remarks:
95% confidence interval: 3.239 – 4.918 µg/L
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
15.507 µg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: Recalculation with ToxRat Professional Vers. 2.10.05
Remarks:
95% confidence interval: 12.986 - 19.548
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
1 other: mg a.i./L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other:
Remarks:
calculation from 2002 (The SAS System for Windows", Version 8.02)
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
16 other: mg a.i./L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: calculation from 2002 (The SAS System for Windows", Version 8.02)
Remarks:
95% confidence interval: 13 - 18 mg a.i./L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The derived NOEC (72 h) was 1.0 mg a.i./L and the ErC50 (72 h) was 16 mg a.i./L (arithmetic mean measured;calculation from 2002 (The SAS System for Windows", Version 8.02)).
The recalculated (ToxRat Professional Vers. 2.10.05) ErC10 (72 h) is 2.050 mg/L and Er50 (72h) is 15.507 µg/L.