2-ethylhexyl 10-ethyl-4-[[2-[(2-ethylhexyl)oxy]-2-oxoethyl]thio]-7-oxo-8-oxa-3,5-dithia-4-phosphatetradecanoate 4-oxide

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

1981

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

1981

Deviations:

yes

Remarks:

Acclimation of fish was 10 days

GLP compliance:

not specified

Specific details on test material used for the study:

TEST MATERIAL (as stated in study report): TK 12 184; trade name D 16-51

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Sponsor / Batch No. Mixt. 4/5/6

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room-temperature (20 - 24 deg C)
- Stability under test conditions:
- Solubility and stability of the test substance in the solvent/vehicle:
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium:

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing:
- Preliminary purification step (if any):
- Final dilution of a dissolved solid, stock liquid or gel:
- Final preparation of a solid:

FORM AS APPLIED IN THE TEST (if different from that of starting material)

TYPE OF BIOCIDE/PESTICIDE FORMULATION (if applicable)

OTHER SPECIFICS:

Analytical monitoring:

no

Vehicle:

yes

Remarks:

acetic acid ethyl ester

Details on test solutions:

10 g TK 12 184 were dissolved in 50 ml Acetic acid-ethylester and completed with water to 1000 ml; this emulsion was treated during 30 seconds with ultrasound. Calculated amounts of stock solution to produce the desired test concentrations were given into the water of the aquaria and homogenously distributed with a high speed mixer. A stable emulsion of the test substance was obtained.

Controls: water control, and vehicle control (0.29 mL/L acetic acid ethyl ester, concentration used for the highest test concentration)

Test organisms (species):

other: Salmo gairdneri

Details on test organisms:

TEST ORGANISM
- Source: H. Mändli, Orishof, CH-4410 Liestal
- Length at study initiation: 60.6 mm (52 - 65 mm)
- Weight at study initiation: 1.79 g (1.26 - 2.4 g)

ACCLIMATION
- Acclimation period: 10 days

FEEDING DURING TEST : none

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

176.56 mg/L CaCO3

Test temperature:

15 +/- 2 deg C

pH:

7.8 - 8.3

Dissolved oxygen:

4.9 - 9.8 mg/L

Nominal and measured concentrations:

5.8, 10, 18, 32, 58 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass aquaria, 15 1 water
- Aeration: none
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 0.6 g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated tap water

OTHER TEST CONDITIONS
- Lighting: indirect daylight , 12 hours photoperiod daily

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality, observations and symptoms were recorded daily

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

8.25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% confidence limits

Remarks:

6.91 - 9.86 mg/L

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

<= 5.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 1 of 10 organisms dead at 96 h

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

18 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No mortality occurred in the water control, or in the vehicle control groups.

Validity criteria fulfilled:

not specified

Conclusions:

Under these guideline test conditions, the LC50 of TK 12 184 in rainbow trout was calculated to be 8.25 mg/L (95% confidence limits: 6.91 - 9.86 mg/11 mg/L)

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

1982

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

1981

Deviations:

no

GLP compliance:

not specified

Specific details on test material used for the study:

TEST MATERIAL (as stated in study report): TK 12 184; trade name D 16-51

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Sponsor / Batch No. Mixt. 4/5/6

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature (20 - 24 deg C)

Analytical monitoring:

no

Vehicle:

yes

Remarks:

acetic acid ethyl ester

Details on test solutions:

Stock solution: 1 g TK 12 184 were dissolved in 5 ml aceticacid-ethylester, emulsified with 30 ml water, treated during 30 seconds with ultrasound and completed with water to 100 ml. Calculated amounts of stock solution to produce the desired test concentrations were given into the water of the aquaria and homogenously
distributed with a high speed mixer. A stable emulsion was obtained.

Controls: water control, and vehicle control (0.09 mL/L acetic acid ethyl ester, concentration used for the highest test concentration)

Test organisms (species):

other: Brachydanio rerio

Details on test organisms:

TEST ORGANISM
- Source: West-Aquarium D-3422 Bad Lauterberg
- Length at study initiation: 27 mm (20 - 32 mm)
- Weight at study initiation (mean and range, SD): 0.16 g (0.1 - 0.2 g)
- Loading: 0.1 g/L

ACCLIMATION
- Acclimation period: 12 days
- Type and amount of food during acclimation:

FEEDING DURING TEST : none

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

195.6 mg/L CaCO3

Test temperature:

23 +/- 2 deg C

pH:

7.8 - 8.5

Dissolved oxygen:

6.9 - 8.3 mg/L

Nominal and measured concentrations:

Nominal: 1.8, 3.2, 5.8, 10.0, 18.0 mg/L

Details on test conditions:

Water: Dechlorinated tap water
TEST SYSTEM
- Test vessel: Glass aquaria, 15 L water
- Aeration: none
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 0.1 g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water

OTHER TEST CONDITIONS
- Lighting: 16 hours fluorescent light per day

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality, observations and symptoms recorded daily

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

4.05 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95 % confidence limits

Remarks:

3.21 - 5.11 mg/L

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

1.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No mortality occurred in the water control or vehicle control groups.

Validity criteria fulfilled:

not specified

Conclusions:

Under these guideline test conditions, the LC50 of TK 12 184 in Zebrafish was calculated to be 4.05 mg/L (95% confidence limits: 3.21 - 5.11 mg/L)

Reference 3

Endpoint:

short-term toxicity to fish

Type of information:

(Q)SAR

Adequacy of study:

supporting study

Study period:

2018

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification

Principles of method if other than guideline:

Results predicted by US EPA ECOSAR v1.11 model.

GLP compliance:

no

Details on results:

No effects are expected at saturated concentrations in water. The estimated logKow for this substance is greater than the class specific cut-offs for acute fish toxicity, and the predicted effect level exceeds the water solubility of this substance by more than 10x.

Validity criteria fulfilled:

not applicable

Conclusions:

The US EPA ECOSAR v1.11 model predicts no effects at saturated concentrations in water for TGEPO (CAS No. 83547-95-9). The estimated logKow for this substance is greater than the class specific cut-offs for acute fish toxicity, and the predicted effect level exceeds the estimated water solubility of this substance by more than 10x.

Description of key information

The LC50 of water emulsions of TK 12 184 was calculated to be 8.25 mg/L (95% confidence limits: 6.91 - 9.86 mg/11 mg/L) in rainbow trout, and 4.05 mg/L (95% confidence limits: 3.21 - 5.11 mg/L) in Zebrafish. Water emulsions in carrier solvents were prepared due to the low water solubility of the test substance.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

4.05 mg/L

Additional information

The US EPA ECOSAR v1.11 model predicts no effects at saturated concentrations in water for TGEPO (CAS No. 83547-95-9).  The estimated logKow for this substance is greater than the class specific cut-offs for acute fish toxicity, and the predicted effect level exceeds the estimated water solubility of this substance by more than 10x.