Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report date:
1990

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
Version / remarks:
The method was modified according to the EC ring test "Assessment of biodegradability of chemicals in water by manometric respirometry (DG X 1 283/82 REV 5)
Deviations:
not specified
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
[8,20-dihydro-8,20-diphenyl-5,24:12,17-diimino-7,10:22,19-dinitrilodibenz[f,p][1,2,4,9,11,12,14,19]octaazacycloicosinato(2-)-N25,N26,N27,N28]iron
EC Number:
256-526-8
EC Name:
[8,20-dihydro-8,20-diphenyl-5,24:12,17-diimino-7,10:22,19-dinitrilodibenz[f,p][1,2,4,9,11,12,14,19]octaazacycloicosinato(2-)-N25,N26,N27,N28]iron
Cas Number:
50293-39-5
Molecular formula:
C32H18FeN12
IUPAC Name:
[8,20-dihydro-8,20-diphenyl-5,24:12,17-diimino-7,10:22,19-dinitrilodibenz[f,p][1,2,4,9,11,12,14,19]octaazacycloicosinato(2-)-N25,N26,N27,N28]iron
Test material form:
solid

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): 10 1 Laboranlage (Wupperverband)
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
100 mg/L
Based on:
test mat.
Remarks:
ThOD = 1405 mg/g
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
TEST CONDITIONS
- Composition of medium: mineral medium
- Test temperature: 20 ± 1 °C
- Suspended solids concentration: 30 mg/l

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes

TEST SYSTEM
- Test performed in open system: no

SAMPLING
- Sampling frequency: every other day
Reference substance
Reference substance:
aniline

Results and discussion

Test performance:
The positive control gave the appropriate response.
% Degradation
Key result
Parameter:
% degradation (O2 consumption)
Value:
0
Sampling time:
28 d
Remarks on result:
other: not readily biodegradable

BOD5 / COD results

Results with reference substance:
The test result is valid as the reference substance aniline reached a degradation of 84% with the same activated sludge.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Conclusions:
Ready biodegradation was determined acc. OECD 301C with some information lacking. However, given data indicate that the test was properly performed, the positive control gave the appropriate results. Hence, the study is considered to be sufficiently reliable to assess the ready biodegradation of the test item. Biodegradation was determined to be 0% (O2 consumption) after 28 days, hence, the test item is considered to be not readily biodegradable.
Executive summary:

Ready biodegradation of the test item was determined acc. OECD 301C, aniline was the positive control and gave the appropriate results. Biodegradation of the test item was determined to be 0% (O2 consumption) after 28 days, it is not readily biodegradable.