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EC number: 218-451-9 | CAS number: 2155-60-4
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
Based on the results obtained in a study performed in accordance with OECD 301F (Manometric Respirometry Test.), it can be concluded that Dibutyl itaconate is readily biodegradable. This conclusion is supported by BIOWIN calculations.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
The ready biodegradation of the substance was investigated according to OECD guideline 301 F and GLP principles. Test concentration of 37 mg/L was tested in six replicates for 28 days. The biodegradation values calculated from the O2 measurements performed during the test period of 28 days revealed significant biodegradation of the substance. At day 7, 42% biodegradation of the substance was observed, 68% at day 14 and 75% at day 28. Since biodegradation of at least 60% was reached within 10 days of biodegradation, the substance was concluded to be readily biodegradable. In the toxicity control, the substance was found not to inhibit microbial activity. The criteria for acceptability of the test were met and the study is considered to be reliable without restriction. In conclusion, the substance was determined to be readily biodegradable under the conditions of the Manometric Respirometry Test.
The two earlier performed Biodegradation studies (OECD 301B and 301D) are used as supporting evidence. The ready biodegradation of the substance under the conditions of the closed bottle test was investigated according to OECD 301D and GLP principles (reliable with restrictions, as not all specifications of the OECD TG 301 D were met). Test concentrations of 1 and 3 mg/L (2.2 and 6.54 mg ThOD/L) were tested in duplicate during 28 days. The relative biodegradation values calculated from the O2 measurements performed during the test period of 28 days revealed significant biodegradation of the substance. At day 7, 53% and 50% biodegradation of the substance at 1 and 3 mg/L, respectively, which was 63% and 60% at day 14. Since biodegradation of at least 60% was reached within 14 days of biodegradation exceeding 10%, the substance was concluded to be readily biodegradable.
Earlier the ready biodegradation of the substance was investigated according to OECD 301B (modified Sturm test) and GLP principles (reliable with restrictions, as not all validity criteria were met). In this study, the substance was also biodegraded significantly during the test period of 28 days. Only, as the pass level of 60% for ready biodegradability within the 10-day window was not met, it was not designated as readily biodegradable under the conditions of the modified Sturm test.
The substance is an ester that may hydrolyze in water to form butanol. Butanol is volatile and is expected to disappear in the modified Sturm test, while it does not disappear in the closed bottle test and the manometric respiratory test, in which all substances that arise are forced to stay into solution. Based on this and as there is no reason to question the test results obtained with the Manometric Respiratory test and supported by the results the closed bottle test, the substance is concluded to be readily biodegradable. This conclusion is supported by BIOWIN calculations.
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