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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
16 November 2017 to 04 January 2018
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
2004
Deviations:
no
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
2008
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
The test item is listed as 4-PPP (CAS # 20657-49-0) and is a clear amber colored liquid stored at room temperature in the dark.
Analytical monitoring:
yes
Vehicle:
no
Details on test solutions:
Concentrations of the test solution used in the definitive test (0.63 mg/L, 1.25 mg/L, 2.5 mg/L 5.0 mg/L and 10 mg/L) were based on the results from a range-finding test using concentrations of 0.10 mg/L, 1.0 mg/L, 10 mg/L and 100 mg/L. Test solutions were prepared after 24 hour of stirring.
Test organisms (species):
Daphnia magna
Details on test organisms:
The test was carried out using first instar Daphnia magna derived from in-house laboratory cultures.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Test temperature:
20 °C to 22 °C
pH:
7.7 to 7.9
Dissolved oxygen:
8.4 to 9.7 mg O2/L
Nominal and measured concentrations:
Based on the results of the range-finding test the following nominal test concentrations were assigned to the definitive test: 0.63 mg/L, 1.25 mg/L, 2.5 mg/L, 5.0 mg/L and 10 mg/L. Chemical analysis of the test preparations at 0 hours showed measured test concentrations to range from 0.41 to 7.8 mg/L.
Reference substance (positive control):
yes
Remarks:
A positive control (Envigo Study Number LK67NP) used potassium dichromate as the reference item at concentrations of 0.32, 0.56, 1.0, 1.8 and 3.2 mg/L.
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.81 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
0.41 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
LOEC
Effect conc.:
0.85 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mobility
Reported statistics and error estimates:
The EC50 values and associated confidence limits at 24 and 48 hours and the slope of the response curve and its standard error were calculated by Probit analysis using Linear Maximum-Likelihood regression. The Lowest Observed Effect Concentration and the No Observed Effect Concentration at 24 and 48 hours were calculated using the Step-down Cochran-Armitage Test Procedure. All results were calculated using the ToxRat Professional computer software package (TOXRAT).
Validity criteria fulfilled:
yes
Remarks:
The test was considered valid since none of the control daphnids showed immobilisation or other signs of disease or stress and the oxygen concentration at the end of the test was equal to or greater than 3 mg/L in the control and test vessels.
Conclusions:
The test substance did induce acute immobilisation of Daphnia magna at the nominal concentrations tested after 48 h of exposure. Chemical analysis of the test preparations showed thtat the test item concentrations ranged from 0.41 mg/L to 7.8 mg/L at the initial 0 hr measurement. The effects concentrations were based on the 0 hour measured test concentrations and are as follows: 48-h NOEC was 0.41mg/L, the 48-h LOEC was 0.85 mg/L and the 48-hr EC50 was 0.81 mg/L.

Description of key information

The key study was a static acute invertebrate toxicity test on the effects of the substance on the freshwater invertebrate Daphnia magna following OECD Guideline 202.  The test substance did induce acute immobilisation of Daphnia magna at the nominal concentrations tested after 48 h of exposure. Chemical analysis of the test preparations showed that the test item concentrations ranged from 0.41 mg/L to 7.8 mg/L at the initial 0-h measurement. The effects concentrations were based on the 0-h measured test concentrations and are as follows: 48-h NOEC was 0.41mg/L, the 48-h LOEC was 0.85 mg/L and the 48-hr EC50 was 0.81 mg/L. 

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
0.81 mg/L

Additional information