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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2018

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
not specified
GLP compliance:
yes

Test material

Constituent 1
Reference substance name:
Hydrolyzed Quinoa
EC Number:
825-815-6
Cas Number:
227025-12-9
IUPAC Name:
Hydrolyzed Quinoa
Constituent 2
Chemical structure
Reference substance name:
Water
EC Number:
231-791-2
EC Name:
Water
Cas Number:
7732-18-5
Molecular formula:
H2O
IUPAC Name:
Water
Test material form:
liquid
Details on test material:
dark clear liquid

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
no
Details on test solutions:
Due to the lack of toxicity observed in preliminary range-finding tests (0.01 to 1000 mg/l concentrations tested) OECD Limit Tests were conducted using direct addition of material.
100 mg/l

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
SPECIES: Daphnia magna
ACCLIMATION/TEST WATER: Hard Syntetic Freshwater
FEEDING PRIOR TO TEST: YCT + Selenastrum capricornutum
FEEDING DURING TEST: None -YCT + Selenastrum 2+ h before test
SOURCE: CI Stock cultures
ACCLIMATION TEMP (°C ): 20
BROOD RELEASE START DATE & TIME: 3/30/18 16:00
BROOD RELEASE END DATE & TIME: 3/31/18 10:30
DATE/TIME WATER ADDED: 3/31/18 13:59
DATE/TIME ANIMALS ADDED: 3/31/18 14:17
ANIMAL AGE WINDOW: 18h 30m
MAX AGE AT TEST START: 22h 17m

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Test temperature:
19 - 21
pH:
8.00 - 8.28
Dissolved oxygen:
7.2 - 8.9 mg/L
Conductivity:
568 - 579 uS/cm
Reference substance (positive control):
yes
Remarks:
KCl

Results and discussion

Effect concentrations
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The EC50 was greater than the test concentration of 100 mg/l.