Hydrolyzed Quinoa

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

not specified

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Hydrolyzed Quinoa

EC Number:

825-815-6

Cas Number:

227025-12-9

IUPAC Name:

Hydrolyzed Quinoa

Constituent 2

Reference substance name:

Water

EC Number:

231-791-2

EC Name:

Water

Cas Number:

7732-18-5

Molecular formula:

H2O

IUPAC Name:

Water

Test material form:

liquid

Details on test material:

dark clear liquid

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

no

Details on test solutions:

Due to the lack of toxicity observed in preliminary range-finding tests (0.01 to 1000 mg/l concentrations tested) OECD Limit Tests were conducted using direct addition of material.
100 mg/l

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

SPECIES: Daphnia magna
ACCLIMATION/TEST WATER: Hard Syntetic Freshwater
FEEDING PRIOR TO TEST: YCT + Selenastrum capricornutum
FEEDING DURING TEST: None -YCT + Selenastrum 2+ h before test
SOURCE: CI Stock cultures
ACCLIMATION TEMP (°C ): 20
BROOD RELEASE START DATE & TIME: 3/30/18 16:00
BROOD RELEASE END DATE & TIME: 3/31/18 10:30
DATE/TIME WATER ADDED: 3/31/18 13:59
DATE/TIME ANIMALS ADDED: 3/31/18 14:17
ANIMAL AGE WINDOW: 18h 30m
MAX AGE AT TEST START: 22h 17m

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Test temperature:

19 - 21

pH:

8.00 - 8.28

Dissolved oxygen:

7.2 - 8.9 mg/L

Conductivity:

568 - 579 uS/cm

Reference substance (positive control):

yes

Remarks:

KCl

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The EC50 was greater than the test concentration of 100 mg/l.