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EC number: 210-762-8 | CAS number: 622-97-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 980
- Report date:
- 1980
Materials and methods
- Principles of method if other than guideline:
- Two groups of 5 male and 5 female rats were exposed (whole body) to the test substance at nominal concentrations of 3300 or 2280 ppm. Mean analysed chamber concentrations were 1960 and 1510 ppm, respectively (equivalent to ca. 7287.53 and 9459.3 mg/m3, respectively). After exposure, the animals were observed for 14 d.
- GLP compliance:
- no
- Limit test:
- no
Test material
- Reference substance name:
- Vinyltoluene
- EC Number:
- 246-562-2
- EC Name:
- Vinyltoluene
- Cas Number:
- 25013-15-4
- Molecular formula:
- C9H10
- IUPAC Name:
- Ethenylmethylbenzene
- Test material form:
- liquid
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- - Source: Charles River, Wilmington, USA
- Weight at study initiation:
Group 1: Males, 235-294 g; Females, 217-236 g
Group 2: Males, 244-294 g; Females, 238-258 g
- Fasting period before study: Not specified
- Acclimation period: 2-3 weeks
Administration / exposure
- Route of administration:
- inhalation: vapour
- Type of inhalation exposure:
- whole body
- Vehicle:
- air
- Details on inhalation exposure:
- Two 6 h inhalation exposures were performed. In each exposure, the test substance was placed in a 1 L nipple bottom bubbler and suspended in awaterbath maintained at 72°C. Nitrogen, at flow rates of 20 and 13 L/minute for Groups 1 and 2, respectively, was passed through a 13 m coil of copper tubing (also immersed in the waterbath) and into the bubbler to create a vapour. The vapour-laden airstream then passed through a kjeldahl trap and through a 1 L round-bottomed trap flask before entering a 760 L glass and stainless steel exposure chamber housing the test animals.
Oxygen was pumped into the chamber at 4 L/minute to maintain the oxygen content of the chamber air at ca 20%. The chamber flow was 125 L/minute.
Additional test substance was delivered into the nipple bottom bubbler from a 1L Erlenmeyer reservoir flask when needed using a pump. A similar pump was used to drain test substance from the 1 L trap flask and deliver it into the Erlenmeyer reservoir flask.
The test substance, 1 L nipple bottom bubbler, three necked round-bottom flask, and Erlenmeyer reservoir flask, clamps, tubing, and stoppers were weighed before and after each exposure period. The difference in weight represented the amount of test substance delivered during each exposure.
The nominal concentration of the test substance was calculated by dividing the amount of substance delivered by the total air flow through the chamber during each exposure period.
Chamber air concentration was monitored continuously during each exposure using an ambient air analyser and recorded once each hour. Waterbath temperature, nitrogen flow rate, oxygen flow rate, and chamber air flow were also recorded hourly.
Nominal and analysed test substance concentrations:
Group 1: During the exposure, a total of 728.98 g of test substance was delivered in a total volume of 45,000 L of air, yielding a nominal exposure concentration of 16.2 mg/L or 3,300 ppm (mol.wt. 118; 1 mg/L = 207 ppm). Mean analysed chamber concentration was 1,960 ppm (range 1840 - 2055 ppm; n=6).
Group 2: During the exposure, a total of 505.20 g of test substance was delivered in a total volume of 45,000 L of air, yielding a nominal exposure concentration of 11.2 mg/L or 2,280 ppm. Mean analysed chamber concentration was 1,510 ppm (range 1480 - 1570 ppm; n=6). - Analytical verification of test atmosphere concentrations:
- yes
- Duration of exposure:
- 6 h
- Concentrations:
- Group 1 (high dose): 3300 ppm (nominal); 1960 ppm (mean analysed)
Group 2 (low dose): 2280 ppm (nominal); 1510 ppm (mean analysed) - No. of animals per sex per dose:
- 5 males and 5 females
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 d
- Frequency of observations and weighing:
Clinical signs: 15 min intervals during the first hour of exposure, then hourly for the remainder of the exposure; on removal of the animals from the chamber; hourly for 2 h post-exposure; and daily thereafter for 14 d.
Body weights: Day 0 (prior to exposure) and on days 1, 2, 4, 7 and 14.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, organ weights, histopathology, other: any animals dying prematurely were subject to necropsy and macroscopic examination as soon as possible after death. On day 14, all surviving animals were killed (ethyl ether) and a macroscopic examination was performed at necropsy.
Results and discussion
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- > 1 510 ppm
- Based on:
- test mat.
- Exp. duration:
- 6 h
- Remarks on result:
- other: i.e. >9459.3 mg/m3 air
- Mortality:
- Group 1 (high dose): One male died on day 1.
Group 2 (low dose): There were no mortalities. - Clinical signs:
- other: Group 1 (high dose): During exposure, all animals showed excessive lachrymation, reduced activity and closed eyes. Excessive salivation and laboured breathing were observed frequently. Other signs mainly included gasping, ataxia, rapid breathing, shallow
- Body weight:
- Group 1 (high dose): Small transient weight losses were noted in all animals; body weights recovered by day 7 for most animals.
Group 2 (low dose): Small transient weight losses were noted in all animals; body weights recovered by day 7 for males, and by day 7/14 in females. - Gross pathology:
- Groups 1 and 2: There were no remarkable macroscopic findings at necropsy.
Applicant's summary and conclusion
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- Under the study conditions, ocular and nasal irritation and neuromuscular impairment were noted after exposure to both concentrations, but these signs abated within 2 d of exposure at the high concentration, and within one day at the low concentration. One rat exposed to the high concentration died on Day 1. Slight, but transient body weight losses were noted for both sexes at each concentration. Macroscopic findings at necropsy were unremarkable for both groups. The 6 h LC50 was therefore >1510 ppm (i.e. >9459.3 mg/m3 or 9.5 mg/L).
- Executive summary:
A study was conducted to determine the acute inhalation toxicity of the test substance in Sprague-Dawley rats. Two groups of 5 male and 5 female rats were exposed (whole body) for 6 h at nominal concentrations of 3300 or 2280 ppm. Mean analysed chamber concentrations were 1960 and 1510 ppm, respectively (equivalent to ca. 7287.53 and 9459.3 mg/m3, respectively). After exposure, the animals were observed for 14 d. Mortality and clinical signs were recorded frequently during and immediately after exposure and daily thereafter. Body weights were determined on Day 0 (prior to exposure) and on Days 1, 2, 4, 7 and 14. At necropsy, a macroscopic examination was performed on all animals. Under the study conditions, ocular and nasal irritation and neuromuscular impairment were noted after exposure to both concentrations, but these signs abated within 2 d of exposure at the high concentration, and within one day at the low concentration. One rat exposed to the high concentration died on Day 1. Slight, but transient body weight losses were noted for both sexes at each concentration. Macroscopic findings at necropsy were unremarkable for both groups. The 6 h LC50 was therefore >1510 ppm (i.e. >9459.3 mg/m3 or 9.5 mg/L) (Norvell, 1980).
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