C10-14-alkyl-1-(ethenyloxy)-

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

20 September to 12 October 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Kyoeisha; Bx 7080101
- Expiration date of the lot/batch: 01 Aug 2019
- Purity test date: 01 aug 2017

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature
- Stability under test conditions: Stable
- Solubility and stability of the test substance in the solvent/vehicle:
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium:

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: none
- Preliminary purification step (if any):
- Final dilution of a dissolved solid, stock liquid or gel:
- Final preparation of a solid:

Analytical monitoring:

yes

Details on sampling:

- Concentrations:
- Sampling method: 50 mL test samples from each replicate were withdrawn and mixed together for each group at 0 and 48 h. The samples were divided into two equal portions. One portion (100 mL) was sent for test concentration analysis and the second portion (100 mL) was stored at -20 ± 5 ºC until study completion. Active ingredient concentration in water was determined using a validated analytical method
- Sample storage conditions before analysis:

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:
- Eluate:
- Differential loading: 1.81
- Controls: Yes
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): none
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)):
- Evidence of undissolved material (e.g. precipitate, surface film, etc.):
- Other relevant information:

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: daphnia
- Strain/clone:
- Age at study initiation (mean and range, SD): less than 24h
- Stage and instar at study initiation: 2nd instar
- Method of breeding: no data
- Source:
- Age of parental stock (mean and range, SD): < 24h
- Feeding during test No
- Food type:
- Amount:
- Frequency:

ACCLIMATION
- Acclimation period: 48h
- Acclimation conditions (same as test or not):
- Type and amount of food: live algal cells
- Feeding frequency:
- Health during acclimation (any mortality observed):

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

no data

Test temperature:

20 ± 1 °C

pH:

no data

Dissolved oxygen:

no data

Salinity:

N/A

Conductivity:

no dats

Nominal and measured concentrations:

0.9, 1.7, 3.1 and 5.5 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass beaker 600mL
- Type (delete if not applicable): open / closed no data
- Material, size, headspace, fill volume:
- Volume of solution: 100mL
- Aeration: none
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4
- Biomass loading rate:

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: in-house
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Salinity:
- Culture medium different from test medium:
- Intervals of water quality measurement:

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 16h light: 8h dark.
- Light intensity: 1320 to 1340 Lux.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

VEHICLE CONTROL PERFORMED: yes/no

RANGE-FINDING STUDY
- Test concentrations:
- Results used to determine the conditions for the definitive study:

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.523 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

0.235 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

0.235 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities:
- Observations on body length and weight: not applicable
- Other biological observations: none
- Mortality of control: none
- Other adverse effects control: none
- Immobilisation of control: Immobilisation of the control was 0%
- Abnormal responses:
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium:

Results with reference substance (positive control):

- Results with reference substance valid? Yes
- Relevant effect levels:
- Limit test: no
- Dose-response test:
- ECx: EC50 0.43 mg/l
- Other:

Validity criteria fulfilled:

yes

Conclusions:

The EC50 was found to be 0.523 mg/L.

Description of key information

The EC50 was found to be 0.523 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.523 mg/L