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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
other: HPV report
Title:
Unnamed
Year:
2003

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Piperazine-1,4-diethanol
EC Number:
204-586-0
EC Name:
Piperazine-1,4-diethanol
Cas Number:
122-96-3
Molecular formula:
C8H18N2O2
IUPAC Name:
1,4-Bis(2-hydroxyethyl)piperazine

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female

Administration / exposure

Vehicle:
water
Doses:
14,700, 18,400, 23,800 and 25,000 mg/kg.
No. of animals per sex per dose:
5

Results and discussion

Effect levelsopen allclose all
Key result
Sex:
female
Dose descriptor:
LD50
Effect level:
18 738 mg/kg bw
Key result
Sex:
male
Dose descriptor:
LD50
Effect level:
20 093 mg/kg bw
Mortality:
At 14,700 mg/kg, 1/5 males and 0/5 females died; at 18,400 mg/kg, 1/5 males and 4/5 females died; at 23,800 mg/kg, 3/5 males and 3/5 females died; at 25,000 mg/kg, all animals died.
Clinical signs:
Clinical symptoms appeared approximately 1-4 hours after dosing. Clinical signs included: piloerection, apathy, passivity, twitching, hematuria and diarrhea. In some animals these symptoms progressed into a coma-like state followed by death.
Animals which survived the 72 hour period after dosing slowly recovered by day 5 and appeared normal at the end of the 14-day study period.

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met
Conclusions:
The rat oral LD50 was 20,093 and 18,738 mg/kg for males and females respectively.