Piperazine-1,4-diethanol

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Data source

Reference

Reference Type:

other: HPV report

Title:

Unnamed

Year:

2003

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Piperazine-1,4-diethanol

EC Number:

204-586-0

EC Name:

Piperazine-1,4-diethanol

Cas Number:

122-96-3

Molecular formula:

C8H18N2O2

IUPAC Name:

1,4-Bis(2-hydroxyethyl)piperazine

Method

Species / strain

Species / strain / cell type:

bacteria, other: Salmonella typhimurium strains TA98, TA100, TA1535 and TA1537 and E. coli strain WP2 uvrA

Metabolic activation:

with and without

Test concentrations with justification for top dose:

100, 333, 1000, 3333 and 5000 ug/plate

Controls

Untreated negative controls:

yes

Positive controls:

yes

Positive control substance:

9-aminoacridine

2-nitrofluorene

sodium azide

methylmethanesulfonate

other: 2 -aminoanthracene

Results and discussion

Test results

Key result

Species / strain:

other: Salmonella typhimurium strains TA98, TA100, TA1535 and TA1537 and E. coli strain WP2 uvrA

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Untreated negative controls validity:

valid

Positive controls validity:

valid

Applicant's summary and conclusion

Conclusions:

Based on the Ames test, DiHEP was not mutagenic, in any strain with or without metabolic activation.