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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
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- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
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- pH
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- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
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- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
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- Nanomaterial surface chemistry
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- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Genetic toxicity
- Carcinogenicity
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- Specific investigations
- Exposure related observations in humans
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- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From Januray 20, 2016 to January 26, 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Quality Assesment Statement ia at page 4 of the report
- Specific details on test material used for the study:
- Batch no.: 145
Dry matter: 37.6% in water (water to 100%)
Purity: 90.4% on the dry matter
Solubility: soluble in water
Being the report done before ECHA Agreement on substance name, the name on the report is L-Glutamic acid, N-(1-oxooctyl)-,sodium salt (1:2) but the substance tested is the reference substance. All raw materials used and manufacturing process are the same - Analytical monitoring:
- yes
- Remarks:
- Measurement of analytical total organic carbon (TOC) concentrations with a TOC analyser
- Details on sampling:
- - Samples, properly diluted with deionised water with low TOC contents, have been analysed by means of an integrated sampling system directly from vials containing 40 ml volume. For each sample under test the corresponding blank is analysed.
- Calculations:
% stability: (TOC substance 72h - (TOC ctl 72h - TOC deionized water 72h))/
(TOC substance 0h - (TOC ctl 0h - TOC deionized water 0h)) * 100
Samples are considered stable if the % stability is between 80 and 120% of the value.
- The samplings were stable throughout the test period (86%, 104%, 95% as indicated in the table 4 Attachment 2 page 14 or the report) - Vehicle:
- no
- Remarks:
- Substance is alreday in water solution
- Details on test solutions:
- Deionized water + dissolved nutrients
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Algal suspension kept in medium culture in sterile 250 mL Erlenmeyer
Starting cellular density: 10E+04
Number of replicates: 6 for control and 3 for treated
Temperature: 23 +/- 2°C
Lighting: > 4300 lux
Stirring: steady with electrical stirrer - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Test temperature:
- 23 °C
- pH:
- 7.53-8.62
- Nominal and measured concentrations:
- Range finding test: 0.1 - 1000 mg/L
Definitive test: 1000 mg/L - Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 173.31 other: mg/l
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Validity criteria fulfilled:
- yes
- Remarks:
- Quality criteria are reported in the Attachment 2 of the report enclosed. All they are satisfied. Growth rate > 0.92% (results 2.02, 1.93, 1.84) %CVss< 35% (results 2.25, 4.11,3.13) %CVwp< 7% (result 0.68)
- Conclusions:
- Under the study conditions, the 72 h EC50 of the substance in Pseudokirchneriella subcapitata was determined to be 173.31 mg a.i./L.
- Executive summary:
A study was conducted to determine the toxicity of the substance to aquatic algae and cyanobacteria according to OECD Guideline 201, in compliance with GLP. Based on a range-finding study, Pseudokirchneriella subcapitata were exposed to test substance concentrations of 0, 95.26, 171.47, 308.64, 555.55 and 1000 mg/L for 72 h. Total organic carbon (TOC) concentrations were measured with a TOC analyser and the test substance was considered stable over the duration of the test. The average growth rate as well as the percent of inhibition of growth rate were calculated. Under the study conditions, the 72 h EC50 of the substance in Pseudokirchneriella subcapitata was determined to be 173.31 mg a.i./L (Giarei, 2016).
Reference
Quality criteria are reported in the Attachment 2 of the report enclosed. All they are satisfied.
Growth rate > 0.92% (results 2.02, 1.93, 1.84)
%CVss< 35% (results 2.25, 4.11,3.13)
%CVwp< 7% (result 0.68)
Interpretation of results:
The average growth rate as well as the percent of inhibition of growth rate were calculated.
Test substance EC50:
The value has been calculated by EPA Probit Program, version 1.5.
Description of key information
A study was conducted to determine the growth inhibition concentration of the substance to Algae according to OECD Guideline 201, in compliance with GLP.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 173.31 mg/L
Additional information
A study was conducted to determine the toxicity of the substance to aquatic algae and cyanobacteria according to OECD Guideline 201, in compliance with GLP. Based on a range-finding study, Pseudokirchneriella subcapitata were exposed to test substance concentrations of 0, 95.26, 171.47, 308.64, 555.55 and 1000 mg/L for 72 h. Total organic carbon (TOC) concentrations were measured with a TOC analyser and the test substance was considered stable over the duration of the test. The average growth rate as well as the percent of inhibition of growth rate were calculated. Under the study conditions, the 72 h EC50 of the substance in Pseudokirchneriella subcapitata was determined to be 173.31 mg a.i./L (Giarei, 2016).
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