Reaction products of phosphoryl trichloride and 2-methyloxirane

Long-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

May-June 1995

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

GLP

Qualifier:

according to guideline

Guideline:

other: OECD Test Guideline 202 (1984)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

The test material was supplied by the study Sponsor as follows:
- Common name: TCPP
- Product name: TCPP = Fyrol PCF
- Purity >99.5%
- Description: clear colourless liquid

Analytical monitoring:

yes

Details on sampling:

Determinations on test concentrations at 0, 2, 5, 7, 9, 12, 14, 16, 19, and 21 days. Samples were taken from the control and each surviving test group (replicates pooled).

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna (Straus) were maintained in a laboratory culture originating from a strain supplied by the Institut National de Recherche Chimique
Appliquee (I .R.CH.A.) France.
Adult Daphnia were maintained in polypropylene vessels containing approximately 2 litres of reconstituted water at a temperature of 21°C.
The lighting cycle was controlled to give a 16 hours light and 8 hours darkness cycle. Fed daily with a suspension of mixed algae.

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Hardness:

approximately 270 mg/L

Test temperature:

21.0

pH:

7.6 - 7.9

Dissolved oxygen:

7.9 - 8.6

Nominal and measured concentrations:

nominal test concentrations of : 10, 18, 32, 56 and 100 mg/L
Measured test concentrations were all in excess of the required 80% of nominal values with the exception of the 32 mg/L test group on days 2, 9, 16, 19 and 21 which showed measured concentrations of 75, 75, 75, 76 and 79 percent of nominal respectively. These low values were considered to be due to analytical and /or sampling variation given that the measured concentrations on all other sampling occasions were near nominal.

Reference substance (positive control):

no

Key result

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

32 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks:

parent

Key result

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

32 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

40 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

immobilisation

Remarks:

parent

Details on results:

The overall mean measured concentrations were shown to range from 85 - 102% of nominal therefore it was considered justifiable to
calculate EC50 values in terms of nominal test concentrations only.

Reported statistics and error estimates:

The EC50 (immobilisation) values and associated confidence limit were calculated by the moving average method of Thompson (1947). The numbers of young produced per adult for the control and each test group were compared using one way analysis of variance (ANOVA) incorporating an Fmax test for homogeneity of variance and the Williams test for differences between treatment means when several dose levels are compared with a zero dose control.

Table - Surviving Adults During the Test

Day	Nominal Concentration (mg/L)
	0	10	18	32	56	100
1	40	40	40	40	40	40
2	40	40	40	40	40	40
3	40	40	40	40	40	40
4	40	40	40	40	39	38
5	40	40	40	40	30	17
6	40	40	40	40	27	7
7	40	40	40	40	23	3
8	40	40	40	40	7	1
9	40	40	40	40	6	0
10	40	40	40	40	4	0
11	40	40	40	40	1	0
12	40	40	40	40	0	0
13	40	40	40	40	0	0
14	40	40	40	40	0	0
15	40	40	40	40	0	0
16	40	40	40	40	0	0
17	40	40	40	40	0	0
18	40	40	40	40	0	0
19	40	40	40	38	0	0
20	40	40	40	37	0	0
21	40	40	40	37	0	0

Table - Total Cumulative Production of Young

Nominal Concentration (mg/L)	Day
	0	2	5	7	9	12	14	16	19	21
0	0	0	0	54	282	715	973	1324	1684	1973
10	0	0	0	35	242	710	1018	1390	1775	2137
18	0	0	0	36	234	667	941	1271	1632	1927
32	0	0	0	41	257	672	921	1199	1514	1862
56	0	0	0	0	0	A/D	-	-	-	-
100	0	0	0	0	A/D	-	-	-	-	-

 

Validity criteria fulfilled:

yes

Remarks:

Control mortality /= 60 %; First young produced within 9 days; cumulative young per female >/= 20 after 14 days and >/= 40 after 21 days; Number of broods per control>/= 3

Conclusions:

No impairment of reproduction was observed at the lower test concentrations of 10, 18 and 32 mg/l TCPP. The "No Observed Effect Concentration" (NOEC) of TCPP is therefore given as 32 mg/l.

Executive summary:

The chronic toxicity of TCPP to aquatic invertebrates (Dapnia magna) was conducted in a semistatic reproduction test according OECD Guideline for Testing of Chemicals 202 (1984). After 21 days of exposure a NOEC-reporduction of 32 mg/l and EC50-reproduction of 40 mg/l were determined. The results relate to nominal concentration, as the exposure concentrations were confirmed by chemical analysis. The analytical monitoring was performed via gaschromatography with external standard calibration. All validity criteria like control mortality, dissolved oxygen, pH, first young, cumulative young per female and number of broods per control group were fulfilled.

Description of key information

Chronic toxicity of TCPP to aquatic invertebrates (Daphnia magna) was assessed in a reproduction test, performed according the OECD Guideline for Testing of Chemicals 202, (1984). After 21 days of exposure a NOEC for reproduction of 32 mg/l was determined. 

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

32 mg/L

Additional information

The chronic toxicity of TCPP to aquatic invertebrates (Daphnia magna) was conducted in a semistatic reproduction test according OECD Guideline for Testing of Chemicals 202 (1984). After 21 days of exposure a NOEC-reporduction of 32 mg/l and EC50-reproduction of 40 mg/l were determined. The results relate to nominal concentration, as the exposure concentrations were confirmed by chemical analysis. The analytical monitoring was performed via gaschromatography with external standard calibration. All validity criteria like control mortality, dissolved oxygen, pH, first young, cumulative young per female and number of broods per control group were fulfilled.