Reaction products of phosphoryl trichloride and 2-methyloxirane

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: This study was conducted according to the methods of Annex V, Part B.1. (acute toxicity (oral), to Directive 67/548/EEC of the Council of the European Communities of June 27, 1967 (Official Journal of the European Communities L196/1 of August 16, 1967)

Principles of method if other than guideline:

This study was conducted according to the methods of Annex V, Part B.1. (acute toxicity (oral), to Directive 67/548/EEC of the Council of the European Communities of June 27, 1967 (Official Journal of the European Communities L196/1 of August 16, 1967) in the current version as amended for the seventeenth time by Directive 92/69/EEC of the Commission of the European Communities of July 31, 1992 (Official Journal of the European Communities L 383 A of December 29, 1992).

GLP compliance:

yes

Test type:

other: Acute oral toxicity

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

1-chloropropan-2-yl bis(2-chloropropyl) phosphate; bis(1-chloropropan-2-yl) 2-chloropropyl phosphate; tris(1-chloropropan-2-yl) phosphate; tris(2-chloropropyl) phosphate

EC Number:

807-935-0

Cas Number:

1244733-77-4

Molecular formula:

C9H18Cl3O4P

IUPAC Name:

1-chloropropan-2-yl bis(2-chloropropyl) phosphate; bis(1-chloropropan-2-yl) 2-chloropropyl phosphate; tris(1-chloropropan-2-yl) phosphate; tris(2-chloropropyl) phosphate

Specific details on test material used for the study:

Tris(2-chlorisopropyl)phosphat CAS-No. 13674-84-5
designation: tris(2-chloropropyl)phosphate
manufacturer: Bayer AG
Batch: 07026
CAS no: 13674-84-5
appeartance: colourleaa liquid
storage: room temperature
expiry date: 07 Feb 1998

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: corn oil

Doses:

200, 500 mg/kg (both sexes) 2000mg/kg females only

No. of animals per sex per dose:

5/sex/dose
only female rats received 2000 mg/kg bw.

Control animals:

not specified

Results and discussion

Effect levels

Effect levels 1

Sex:

female

Dose descriptor:

LD50

Effect level:

632 mg/kg bw

Effect levels 2

Sex:

male

Dose descriptor:

LD50

Effect level:

> 500 - < 2 000 mg/kg bw

Mortality:

All animals in the female group 2000 mg/kg bw died after 3-6 hours.

Clinical signs:

other: other: In female rats after 2000 mg/kg bw following signs of intoxication were observed: Apathy, palmospasm, blood-crusted snout and lateral position. A single dose of 200 and 500 mg/kg bw was tolerated by male and female rats without any clinical signs.

Gross pathology:

At necropsy mottled reddened lungs were noticed in the animals which died during the observation period.
None of the animals sacrified at the end of the 14-day observation period showed any noticeable gross pathological findings.

Any other information on results incl. tables

RS-Freetext:
LD50 > 500 mg/kg (males); LD50 = 632 mg/kg (females)

RS-Freetext:
mortality: 0/5 at 200 and 500 mg/kg (both sexes), 5/5 at
2000 mg/kg (females); clinical signs observed only at 2000
mg/kg: apathy, palmospasm, blood-crusted snout and lateral
position; necropsy findings only at 2000 mg/kg: reddened
lungs

Applicant's summary and conclusion

Executive summary:

Acute toxicological investigations in male and female Wistar rats were conducted after single oral administration of TCPP.

The LD50 was calculated as approximate 632 mg/kg body weight for female rats and was greater than 500 mg/kg and less than 2000 mg/kg bw for the male rats.