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EC number: 619-232-4 | CAS number: 96624-52-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
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- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
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- Endpoint summary
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
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- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
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- Endocrine disrupter testing in aquatic vertebrates – in vivo
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Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Jul 04 - Sep 17, 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- 22 Oct 2010
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Version / remarks:
- 30 May 2008
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- [trans(trans)]- 4-Methyl-4'-propyl-1,1'-bicyclohexyl
- EC Number:
- 816-312-2
- Cas Number:
- 92343-70-9
- Molecular formula:
- C16H30
- IUPAC Name:
- [trans(trans)]- 4-Methyl-4'-propyl-1,1'-bicyclohexyl
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Appropriate amounts of test item were directly weight into the test vessels and test water was added. The composition was stirred intensively
- Controls: pure water, synthetic sewage and inoculum, but without addition of test item
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Test System: Activated sludge
Origin: Municipal sewage treatment plant of Darmstadt, Germany
Reason for the selection: Non-adapted activated sludge from the sewage plant at Bensheim is well suited as it receives predominantly municipal sewage and hardly any industrial chemical waste.
Pretreatment: The activated sludge used for this study was used as collected, but coarse particles were removed by settling for a short period (15 minutes) and then the upper layer decanted.
During holding prior to use the sludge was fed with 50 mL synthetic sewage feed (see below) per litre and kept aerated at room temperature overnight. An aliquot of the final sludge suspension was weighed, dried
and the ratio of wet sludge to its dry weight determined.
Based on the sludge dry matter, calculated amounts of wet sludge were suspended in pure water to yield a concentration equivalent to 3 g/L on dry weight basis. This level gives a concentration of 1.5 g/L suspended solids in the
test medium. The pH of the activated sludge inoculum was 6.9 and therefore no adjustment necessary.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 20.4°C
- pH:
- control: 7.3 (start) to 8.6 (end)
test item: 7.3 (start) to 8.6 (end) - Dissolved oxygen:
- Control samples : 6.9 mg O2/L
- Nominal and measured concentrations:
- test item: 100 mg/L (nominal)
positive control: 1, 4 and 16 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass flasks (approx. 1 litre) and Karlsruher flasks (250 mL)
- Type: open
- Aeration:compressed air (1.017 litre per minute)
- No. of vessels per test item concentration (replicates): 1
- No. of vessels per positive control concentration (replicates): 1
- No. of vessels of control (replicates): 2
- Sludge concentration (weight of dry solids per volume): 3.5 g/L
- Nutrients provided for bacteria: no
- Nitrification inhibitor used: N-allylthiourea
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionized water
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
TEST CONCENTRATIONS
- Test item concentrations: 100 mg/L - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- At a nominal test concentration of 100 mg test item/L less than 10% inhibition was observed after three hours of
incubation. The mean deviation in respiration rate was 4.2% at three replicates of 100 mg Art. 201498.
The respiration rate of the activated sludge to the test material at the rate of 100 mg/L was not statistically significant reduced when compared to the control (Two
sample t-test procedure). The test material at the rate of 100 mg/L had no toxic effects on respiration activity of activated sludge.
Therefore it can be assumed that the NOEC is above 100 mg test item/L. - Results with reference substance (positive control):
- 3,5-Dichlorophenol
EC50 = 8.1 mg/L
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- At a nominal test concentration of 100 mg test item/L less than 10% inhibition was observed after three hours of
incubation. The mean deviation in respiration rate was 4.2% at three replicates of 100 mg test material.
The respiration rate of the activated sludge to the test material at the rate of 100 mg/L was not statistically significant reduced when compared to the control (Two
sample t-test procedure). The test material at the rate of 100 mg/L had no toxic effects on respiration activity of activated sludge.
Therefore it can be assumed that the NOEC is above 100 mg test item/L.
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