Dipentaerythritol hexaesters of 3,5,5-trimethylhexanoic and n-heptanoic acids

Endpoint summary

Description of key information

There is no data available for the skin and eye irritation/corrosion potential of Dipentaerythritol hexaesters of 3,5,5-trimethylhexanoic and n-heptanoic acids (CAS 1379424 -11 -9). In order to fulfil the standard information requirements set out in Annex VIII, 8.5, in accordance with Annex XI, 1.5, of Regulation (EC) No 1907/2006, read-across from a structurally related substance was conducted.

The substance EC 444 -000-2 consists of dipentaerythritol hexaesters with fatty acids n-pentanoic and 3,5,5 -trimethylhexanoic acid and it is considered to be similar to the target substance, on the basis of the structural and similar properties and/or activties.

The available data on irritation/corrosion potential for the source substance (OECD 404 and OECD 405 , non skin irritating and non eye irritating) is used to predict the same behaviour for the target substance Dipentaerythritol hexaesters of 3,5,5 -trimethylhexanoic and n-heptanoic acids (CAS 1379424 -11 -9).

Key value for chemical safety assessment

Skin irritation / corrosion

Link to relevant study records

Reference

Reference 1

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

16 Dec - 23 Dec 1998

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method B.4 (Acute Toxicity: Dermal Irritation / Corrosion)

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: EPA OPPTS 879.2700 (draft)

GLP compliance:

yes (incl. QA statement)

Remarks:

The Department of Health of the Government of the United Kingdom, UK

Species:

rabbit

Strain:

New Zealand White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: David Percival Ltd., Moston Sandbach, Cheshire, UK
- Age at study initiation: 12-16 weeks
- Weight at study initiation: 2.71-2.85 kg
- Housing: individual in suspended metal cages.
- Diet: STANRAB SQC Rabbit Diet (Special Diets Services Ltd., Witham, Essex, UK), ad libitum
- Water: tap water, ad libitum

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 17-20
- Humidity (%): 51-60
- Air changes (per hr): approximately 15
- Photoperiod (hrs dark / hrs light): 12/12

Type of coverage:

semiocclusive

Preparation of test site:

shaved

Vehicle:

unchanged (no vehicle)

Controls:

other: untreated sites of the same animal served as control

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied: 0.5 mL
- Concentration: 100%

Duration of treatment / exposure:

4 h

Observation period:

14 days
Reading time points: 1, 24, 48 and 72 h and 7 days

Number of animals:

3

Details on study design:

TEST SITE
- Area of exposure: dorsal flank
- Type of wrap if used: the test material was introduced under a 6.25 cm² cotton gauze patch and held in position with a strip of surgical adhesive tape (BLENDERM: approximately size 2.5 cm x 4.0 cm). To prevent the animals interfering with the patches, the trunk of each rabbit was wrapped in an elasticated corset (TUBIGRIP).

REMOVAL OF TEST SUBSTANCE
- Washing: residual test material was removed by gentl swabbing with cotton wool soaked in 74% Industrial Methylated Spirits.
- Time after start of exposure: 4 h

SCORING SYSTEM: Draize scoring system

Irritation parameter:

erythema score

Basis:

animal #1

Time point:

24/48/72 h

Score:

1

Max. score:

4

Reversibility:

fully reversible within: 7 days

Irritation parameter:

erythema score

Basis:

animal #2

Time point:

24/48/72 h

Score:

1

Max. score:

4

Reversibility:

fully reversible within: 7 days

Irritation parameter:

erythema score

Basis:

animal #3

Time point:

24/48/72 h

Score:

0.67

Max. score:

4

Reversibility:

fully reversible within: 72 h

Irritation parameter:

edema score

Basis:

mean

Remarks:

out of all 3 animals

Time point:

24/48/72 h

Score:

0

Max. score:

4

Reversibility:

other: reversibility: not applicable

Irritant / corrosive response data:

Erythema: 2/3 animals showed slight erythema from 24 h up to 72 h being fully reversible within 7 days. In the remaining animal slight erythema was observed at the 24 and 48 h reading time point only.
Edema: No edema formation was apparent during the study period in any animal.

Other effects:

No other local or systemic effects were reported.

Table 1. Results of skin irritation study.

Observation time	Rabbit number and body weight (kg)
	#77 (2.83)		#79 (2.85)		#80 (2.71)
	Erythema	Edema	Erythema	Edema	Erythema	Edema
1 h	0	0	0	0	0	0
24 h	1	0	1	0	1	0
48 h	1	0	1	0	1	0
72 h	1	0	1	0	0	0
7 days	0	0	0	0	0	0
Mean value 24 + 48 + 72 h	1.00	0.00	1.00	0.00	0.67	0.00

 

Interpretation of results:

not irritating

Conclusions:

CLP: not classified

Reference 2

Endpoint:

skin irritation: in vivo

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

There are no data available on the skin and eye irritation potential of the substance Dipentaerythritol hexaesters of 3,5,5-trimethylhexanoic and n-heptanoic acids (CAS 1379424 -11 -9). In order to fulfill the standard information requirements set out in Annex VII, in accordance with Annex XI, 1.5, of Regulation (EC) No 1907/2006, a read-across from a structurally related substance was conducted. In accordance with Article 13 (1) of Regulation (EC) No 1907/2006, "information on intrinsic properties of substances may be generated by means other than tests, provided that the conditions set out in Annex XI are met. In particular for human toxicity, information shall be generated whenever possible by means other than vertebrate animal tests", which includes the use of information from structurally related substances (grouping or read-across). Having regard to the general rules for grouping and read-across approach laid down in Annex XI, Item 1.5, of Regulation (EC) No 1907/2006, whereby toxicological properties may be predicted from data for the reference substance(s) on the basis of structural similarity, the substance Dipentaerythritol with fatty acids C5 and C9 iso (formerly identified as CAS 647028-25-9) is selected as reference substance for assessment of toxicological endpoints, for which information gaps are identified.
The source and target substances are considered to be similar on the basis of structural similariy resulting in similar properties and/or activities. The available endpoint information is used to predict the same endpoints for Dipentaerythritol hexaesters of 3,5,5-trimethylhexanoic and n-heptanoic acids (CAS 1379424 -11 -9). A detailed analogue approach justification is provided in the technical dossier (see IUCLID Section 13).

Reason / purpose for cross-reference:

read-across source

Irritation parameter:

erythema score

Basis:

animal #1

Time point:

24/48/72 h

Score:

1

Max. score:

4

Reversibility:

fully reversible within: 7 days

Irritation parameter:

erythema score

Basis:

animal #2

Time point:

24/48/72 h

Score:

1

Max. score:

4

Reversibility:

fully reversible within: 7 days

Irritation parameter:

erythema score

Basis:

animal #3

Time point:

24/48/72 h

Score:

0.67

Max. score:

4

Reversibility:

fully reversible within: 72 h

Irritation parameter:

edema score

Basis:

mean

Remarks:

out of all 3 animals

Time point:

24/48/72 h

Score:

0

Max. score:

4

Reversibility:

other: not applicable

Interpretation of results:

GHS criteria not met

Conclusions:

The source substance Dipentaerythritol wiht fatty acids C5 and C9iso resulted non irritating in a GLP skin irritation study (OECD 404).
Based on the results from the structurally related source substance, it can be concluded that the target substance Dipentaerythritol hexaesters of 3,5,5-trimethylhexanoic and n-heptanoic acids will not exhibit skin irritation /corrosion effects.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed (not irritating)

Eye irritation

Link to relevant study records

Reference

Reference 1

Endpoint:

eye irritation: in vivo

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

There are no data available on the skin and eye irritation potential of the substance Dipentaerythritol hexaesters of 3,5,5-trimethylhexanoic and n-heptanoic acids (CAS 1379424 -11 -9). In order to fulfill the standard information requirements set out in Annex VII, in accordance with Annex XI, 1.5, of Regulation (EC) No 1907/2006, a read-across from a structurally related substance was conducted. In accordance with Article 13 (1) of Regulation (EC) No 1907/2006, "information on intrinsic properties of substances may be generated by means other than tests, provided that the conditions set out in Annex XI are met. In particular for human toxicity, information shall be generated whenever possible by means other than vertebrate animal tests", which includes the use of information from structurally related substances (grouping or read-across). Having regard to the general rules for grouping and read-across approach laid down in Annex XI, Item 1.5, of Regulation (EC) No 1907/2006, whereby toxicological properties may be predicted from data for the reference substance(s) on the basis of structural similarity, the substance Dipentaerythritol with fatty acids C5 and C9 iso (CAS 647028-25-9) is selected as reference substance for assessment of toxicological endpoints, for which information gaps are identified.
The source and target substances are considered to be similar on the basis of structural similariy resulting in similar properties and/or activities. The available endpoint information is used to predict the same endpoints for Dipentaerythritol hexaesters of 3,5,5-trimethylhexanoic and n-heptanoic acids (CAS 1379424 -11 -9). A detailed analogue approach justification is provided in the technical dossier (see IUCLID Section 13).

Reason / purpose for cross-reference:

read-across source

Irritation parameter:

cornea opacity score

Basis:

mean

Remarks:

out of all 3 animals

Time point:

24/48/72 h

Score:

0

Max. score:

4

Reversibility:

other: reversibility: not applicable

Irritation parameter:

iris score

Basis:

mean

Remarks:

out of all 3 animals

Time point:

24/48/72 h

Score:

0

Max. score:

2

Reversibility:

other: reversibility: not applicable

Irritation parameter:

conjunctivae score

Basis:

animal #1

Time point:

24/48/72 h

Score:

0

Max. score:

3

Reversibility:

other: reversibility: not applicable

Irritation parameter:

conjunctivae score

Basis:

animal #2

Time point:

24/48/72 h

Score:

0.33

Max. score:

3

Reversibility:

fully reversible within: 2 days

Irritation parameter:

chemosis score

Basis:

mean

Remarks:

out of all 3 animals

Time point:

24/48/72 h

Score:

0

Max. score:

4

Reversibility:

other: reversibility: not applicable

Irritant / corrosive response data:

No effects on corneal opacity and iris were noted at any time point observed. Moderate conjunctival redness (score = 2) was noted in two treated animals at the 1 h observation. Minimal conjunctival redness (score = 1) was observed in one treated animal at the 1 h observation and persisted in two treated animals to the 24 h reading. Minimal chemosis (score = 1) was observed in all treated animals at the 1 h observation time point. Treated eyes appeared normal 24 to 48 h after treatment.

Other effects:

No further local or systemic effects were reported.

Interpretation of results:

GHS criteria not met

Conclusions:

The source substance Dipentaerythritol wiht fatty acids C5 and C9iso resulted non irritating in a GLP eye irritation study (OECD 405).
Based on the results from the structurally related source substance, it can be concluded that the target substance Dipentaerythritol hexaesters of 3,5,5-trimethylhexanoic and n-heptanoic acids will not exhibit skin irritation /corrosion effects.

Reference 2

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

23 Dec 1998 - 1 Jan 1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Deviations:

yes

Remarks:

no data on analytical purity

Qualifier:

according to guideline

Guideline:

EU Method B.5 (Acute Toxicity: Eye Irritation / Corrosion)

Deviations:

yes

Remarks:

no data on analytical purity

Qualifier:

according to guideline

Guideline:

EPA OPPTS 870.2400 (Acute Eye Irritation)

Deviations:

yes

Remarks:

no data on analytical purity

GLP compliance:

yes (incl. QA statement)

Remarks:

The Department of Health of the Government of the United Kingdom, UK

Species:

rabbit

Strain:

New Zealand White

Details on test animals or tissues and environmental conditions:

TEST ANIMALS
- Source: David Percival Ltd, Moston, Sandbach, United Kingdom
- Age at study initiation: 12-16 weeks
- Weight at study initiation: 2.93-3.13 kg
- Housing: animals were housed individually in suspended metal cages
- Diet: STANRAB SQC Rabbit Diet (Special Diets Services Ltd, Witham, United Kingdom), ad libitum
- Water: mains tap water, ad libitum
- Acclimation period: at least 5 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 17-19
- Humidity (%): 43-64
- Air changes (per hr): 15
- Photoperiod (hrs dark / hrs light): 12/12

Vehicle:

unchanged (no vehicle)

Controls:

other: not required, the untreated eye served as control

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied: 0.1 mL

Duration of treatment / exposure:

single application without washing

Observation period (in vivo):

3 days
Reading time points: 1, 24, 48 and 72 h

Number of animals or in vitro replicates:

1 male and 2 females

Details on study design:

SCORING SYSTEM: Draize scoring system

Irritation parameter:

cornea opacity score

Basis:

mean

Remarks:

out of all 3 animals

Time point:

24/48/72 h

Score:

0

Max. score:

4

Reversibility:

other: reversibility: not applicable

Irritation parameter:

iris score

Basis:

mean

Remarks:

out of all 3 animals

Time point:

24/48/72 h

Score:

0

Max. score:

2

Reversibility:

other: reversibility: not applicable

Irritation parameter:

conjunctivae score

Basis:

animal #1

Time point:

24/48/72 h

Score:

0

Max. score:

3

Reversibility:

other: reversibility: not applicable

Irritation parameter:

conjunctivae score

Basis:

animal #2

Time point:

24/48/72 h

Score:

0.33

Max. score:

3

Reversibility:

fully reversible within: 2 days

Irritation parameter:

conjunctivae score

Basis:

animal #3

Time point:

24/48/72 h

Score:

0.33

Max. score:

3

Reversibility:

fully reversible within: 2 days

Irritation parameter:

chemosis score

Basis:

mean

Remarks:

out of all 3 animals

Time point:

24/48/72 h

Score:

0

Max. score:

4

Reversibility:

other: reversibility: not applicable

Irritant / corrosive response data:

No effects on corneal opacity and iris were noted at any time point observed. Moderate conjunctival redness (score = 2) was noted in two treated animals at the 1 h observation. Minimal conjunctival redness (score = 1) was observed in one treated animal at the 1 h observation and persisted in two treated animals to the 24 h reading. Minimal chemosis (score = 1) was observed in all treated animals at the 1 h observation time point. Treated eyes appeared normal 24 to 48 h after treatment.

Other effects:

No further local or systemic effects were reported.

Interpretation of results:

not irritating

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

CLP: not classified

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed (not irritating)

Respiratory irritation

Endpoint conclusion

Endpoint conclusion:

no study available

Justification for classification or non-classification

Based on read-across from a structurally similar substance, the available data on skin/eye irritation potential do not meet the classification criteria according to Regulation (EC) 1272/2008 and are therefore conclusive but not sufficient for classification.