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Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2018-02-21 - 2018-04-23
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2018
Report date:
2018

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
Deviations:
yes
Remarks:
Ammonium chloride excluded from medium to prevent oxygen consumption as a result of nitrification / river water used as inoculum
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
trinonyl trimellitate
IUPAC Name:
trinonyl trimellitate
Details on test material:
Physical state at 25°C: Liquid
Storage conditions: Keep container tightly closed. Keep in a dry, cool and well ventilated place.
Lot number: 3607211300
Expiry date: The product TM 9 due to its nature does not have expiry date.
Purity: 99.98% w/w
Date received: 15 November 2011
Storage at testing facility: 5 years
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source: Commercial batch
- Lot/batch No.of test material: 2103218004
- Purity: 99.9%

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Ambient conditions, in the dark
- Stability under test conditions: Stable

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: None

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
natural water: freshwater
Details on inoculum:
- Source of inoculum/activated sludge: River water from the Rhine near Heveadorp, The Netherlands. The nearest plant (Arnhem-Zuid) treating domestic wastewater biologically was 3 km upstream of the sampling site.
- Pretreatment: Aerated for 7 days before use to reduce the endogenous respiration. River water without particles was used as inoculum. The particles were removed by sedimentation after 1 day while moderately aerating.
Duration of test (contact time):
60 d
Initial test substance concentration
Initial conc.:
2 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
TEST CONDITIONS
- Composition of medium: As per OECD TG 301 less ammonium chloride (to prevent nitrification)
- Solubilising agent (type and concentration if used): Tween 80, 2.0 mg/L
- Test temperature: 22.6 - 23.0 deg C
- pH: 8.0
- pH adjusted: No
- Continuous darkness: Yes

TEST SYSTEM
- Culturing apparatus: 300 mL BOD bottles
- Number of culture flasks/concentration: 10
- Method used to create aerobic conditions: Aeration of inoculum
- Measuring equipment: Oxygen electrode

SAMPLING
- Sampling frequency: Days 0, 7, 14, 21, 28, 42 and 60

CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Other: Yes - bottles containing inoculum + surfactant

Reference substance
Reference substance:
acetic acid, sodium salt

Results and discussion

% Degradationopen allclose all
Parameter:
% degradation (O2 consumption)
Value:
0
Sampling time:
0 d
Parameter:
% degradation (O2 consumption)
Value:
2
Sampling time:
7 d
Parameter:
% degradation (O2 consumption)
Value:
15
Sampling time:
14 d
Parameter:
% degradation (O2 consumption)
Value:
29
Sampling time:
21 d
Parameter:
% degradation (O2 consumption)
Value:
46
Sampling time:
28 d
Parameter:
% degradation (O2 consumption)
Value:
63
Sampling time:
42 d
Parameter:
% degradation (O2 consumption)
Value:
71
Sampling time:
60 d

BOD5 / COD results

Results with reference substance:
76% degrdation after 14 days

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
inherently biodegradable
Conclusions:
The substance was biodegraded by 46% at day 28 in the Closed Bottle test. In the prolonged Closed Bottle test (enhanced biodegradability test) the substance was biodegraded by 71% within 60 days.
The biodegradation percentage of >60% demonstrates that test substance is inherently biodegradable. Moreover, the biodegradation in excess of 60% within the 60-day test period allows classification of the substance as not persistent
Executive summary:

Biodegradation has been studied in the Closed Bottle test, performed according to slightly modified EEC, OECD and ISO Test methods.

The substance was biodegraded by 46% at day 28 in the Closed Bottle test. In the prolonged Closed Bottle test (enhanced biodegradability test) the substance was biodegraded by 71% within 60 days.

The biodegradation percentage of >60% demonstrates that test substance is inherently biodegradable. Moreover, the biodegradation in excess of 60% within the 60-day test period allows classification of the substance as not persistent