Hexachloroethane

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

1978

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: test performed prior to guideline availability but with procedures similar to standard methods and with acceptable restrictions

Data source

Reference

Reference 1

Reference Type:

other: Published toxicological report

Title:

Unnamed

Year:

2011

Reference 2

Reference Type:

other: Published toxicological report

Title:

Toxicological Profile for Hexachloroethane

Author:

Agency for Toxic Substances and Disease Registry (ATSDR)

Year:

1997

Bibliographic source:

Not reported

Reference 3

Reference Type:

publication

Title:

The toxicity of hexachloroethane in laboratory animals.

Author:

Weeks MH, Angerhofer RA, Bishop R, Thomasino J, Pope CR.

Year:

1979

Bibliographic source:

Am Ind Hyg Assoc J. 1979 Mar;40(3):187-99. doi: 10.1080/15298667991429499. PMID: 495459.

Reference 4

Reference Type:

study report

Title:

Unnamed

Year:

1978

Materials and methods

Test guideline

Qualifier:

no guideline available

Principles of method if other than guideline:

- Principle of test: determine the mutagenicity potential of the test material in bacteria exposed to hexachloroethane with or without metabolic activation, followed by a quantification of revertant colonies.
- Short description of test conditions: in plate assay, S. typhimurium and S. cerevisiae strains were exposed to the test material in DMSO at concentrations up to the induction of cytotoxic effects. Vehicle and positive controls were included.
- Parameters analysed / observed: revertant colonies

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Hexachloroethane

EC Number:

200-666-4

EC Name:

Hexachloroethane

Cas Number:

67-72-1

Molecular formula:

C2Cl6

IUPAC Name:

hexachloroethane

Test material form:

solid: crystalline

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch number of test material: Hummel Chemical Company, specifications MIL-H-235B, national stock number 6810-00-N00-001
- Purity: technical grade, 99.8%

Method

Species / strain

Species / strain 1

Species / strain / cell type:

S. typhimurium, other: TA-1535, TA-1537, TA-1538, TA-98, TA-100

Species / strain 2

Species / strain / cell type:

Saccharomyces cerevisiae

Additional strain / cell type characteristics:

other: D4 strain

Metabolic activation:

with and without

Metabolic activation system:

Liver microsomal enzyme preparation from Sprague Dawley rats pretreated 5 days prior to kill with Aroclor 1254

Test concentrations with justification for top dose:

0.1, 1.0, 10, 100 and 500 µg/plate - The compound was tested over a series of concentrations such that there was either quantitative or qualitative evidence of some chemically induced physiological effects at the high dose level. The low dose in all cases was below a concentration that demonstrated any toxic effect.

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle and percentage of solvent in the final culture medium: not specified

Controls

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

Remarks:

but no details

True negative controls:

no

Positive controls:

yes

Positive control substance:

not specified

Details on test system and experimental conditions:

METHOD OF TREATMENT/ EXPOSURE:
- Plate assay

Results and discussion

Test results

Test results 1

Key result

Species / strain:

S. typhimurium, other: TA-1535, TA-1537, TA-1538, TA-98, TA-100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Test results 2

Species / strain:

Saccharomyces cerevisiae

Remarks:

D4 strain

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Applicant's summary and conclusion

Conclusions:

According to the authors, the test material did not induce any significant increase in revertant colonies with or without metabolic activation, neither in S. tiphymurium nor in S. cerevisiae strains.

Executive summary:

The mutagenicity potential of hexachloroethane was assessed by performing a bacterial reverse mutation assay in S. typhimurium TA-1535, TA-1537, TA-1538, TA-98, TA-100 and S. cerevisiae D4 strains, with or without metabolic activation. There was no information regarding a standardised method or GLP.

The test material was prepared in DMSO solvent and doses of 0.1, 1.0, 10, 100 and 500 µg/plate were applied to bacteria with or without metabolic activation system, i.e. liver microsomal enzyme preparation from Sprague Dawley rats pre-treated 5 days prior to kill with Aroclor 1254. Solvent and positive controls were included in this plate assay.

No result details were available. According to the authors, the test material did not induce any significant increase in revertant colonies with or without metabolic activation, neither in S. typhimurium nor in S. cerevisiae strains.