Ammonium 7-(2,6-dimethyl-8-(2,2-dimethylbutyryloxy)-1,2,6,7,8,8a-hexahydro-1-naphthyl)-3,5-dihydroxyheptanoate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

5th February 1990 to 9th February 1990

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

GLP compliance:

yes

Specific details on test material used for the study:

Composition:
99.2% L-654,969, 0.2% lovastatin ammonium salt, 0.2% triol

Details on sampling:

Water samples were taken from control and test concentrations at 0, 24 and 96 hours

Details on test solutions:

Test concentrations:
32, 56, 100, 180 and 320 mg/L

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow trout
- Source: Westacre Trout Farm, Norfolk, UK.
- Length at study initiation: mean - 4.9cm
- Weight at study initiation: mean - 1.71g

ACCLIMATION
- Acclimation period: 2 months in test conditions
- Type and amount of food during acclimation: commercial trout pellets
- Health during acclimation: <1% mortality in 7 days prior to test

Test type:

semi-static

Water media type:

not specified

Total exposure duration:

96 h

Hardness:

350 mg CaCO3/L

Test temperature:

14°C ± 1°C

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass aquaria holding 20L of test media.
- Aeration: via narrow bore glass tubes
- No. of organisms per vessel: 10 animals per test concentration

OTHER TEST CONDITIONS
- Photoperiod: 16 h light, 8 h dark

- Test concentrations: 32, 56, 100, 180 and 320 mg/L

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

ca. 240 mg/L

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

ca. 220 mg/L

Basis for effect:

mortality (fish)

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

ca. 220 mg/L

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

ca. 220 mg/L

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

ca. 320 mg/L

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

ca. 100 mg/L

Conclusions:

LC50 (96 h) 220 mg/L

Executive summary:

A 96 hour LC₅₀ test was carried out on test substance L654,969 using rainbow trout. The test conditions were semi-static, and according to OECD Guideline 203 and EEC directive 67/548 Annex V C1. 

The resulting LC₅₀ (96 h) is 220 mg/L

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

220 mg/L