[No public or meaningful name is available]

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

17 July 1992

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

Batch Sample Ref: CRKL-719-09-12-VII-003-09

Analytical monitoring:

yes

Details on sampling:

the test solutions were measured at 0 and 96 hours

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A 100 mg/L stock solution was prepared by weighing the required amount of test substance into glass vials. The contents of the vials were then added to dilution water in stock jars and ultrasonicated for 45 minutes. The stock solutions were mixed together. Appropriate amounts of the stock solution were dispensed into the appropriate tanks containing dilution water (volumes adjusted). The test solutions were then stirred. The control tank contained dilution water only. The final solutions were clear and colourless.

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: rainbow trout
- Source: Houghton Springs Fish Farm, Winterbourne Houghton, Blandford Forum, Dorset, DT11 0PD

ACCLIMATION
- Acclimation period: 7 days
- Acclimation conditions (same as test or not): same
- Type and amount of food during acclimation: fed daily using a proprietary brand of high protein pelleted fish food.
- Feeding frequency during acclimation: daily
- Health during acclimation (any mortality observed): There was 1 mortality recorded (1.4%) within the 48 hours prior to the start of the study.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

56 mg/L CaCO3

Test temperature:

15 oC

pH:

6.7 to 7.8

Dissolved oxygen:

9.0 to 10 mg/L

Conductivity:

228 μS cm-1 @ 25oC

Nominal and measured concentrations:

Nominal concentrations: 10, 18, 32, 56 and 100 mg/L
Measured concentrations: 10, 20, 35, 52 and 53 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume:
- Aeration: gentle
- Renewal rate of test solution (frequency/flow rate): no
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates):
- Biomass loading rate: 0.44 g/L

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light: 8 hours dark with 20 minute dawn and dusk transition periods
- Light intensity:

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Observations for mortalities and symptoms of toxicity were made at 3, 24, 48, 72 and 96 hours. At 24 and 48 hours observations of symptoms of toxicity were impeded due to the turbidity of the test solution in the 100 mg/L test concentration.

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

38 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Cumulative percentage mortality observed at 96 h:
Nominal 56 mg/L: 86%
Nominal 100 mg/L: 100%

Validity criteria fulfilled:

yes

Conclusions:

The 96 hours LC50 of the test item based on mortality is determined as 38 mg/L.

Executive summary:

The acute toxicity of the test item to rainbow trout Oncorhynchus mykiss was determined, according to OECD Guideline 203 under GLP. The 96 hours LC50 of the test item based on mortality is determined as 38 mg/L.

Description of key information

The acute toxicity of the test item to rainbow trout Oncorhynchus mykiss was determined, according to OECD Guideline 203 under GLP. The 96 hours LC50 of the test item based on mortality is determined as 38 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

38 mg/L