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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
according to guideline
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
13 April 2004
GLP compliance:
Specific details on test material used for the study:
Batch Sample Ref CRKL-719-09-12-VII-003-09
Analytical monitoring:
Details on sampling:
the test solutions were measured at 0 and 48 hours
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution (and the highest test concentration) of PF-03394197 with a nominal concentration of 56 mg/L was prepared by the addition of a nominal 0.112 g of test substance to dilution water made up to 2000 ml. The stock solution was ultrasonicated for 30 minutes after which it was observed to be a clear and colourless solution.
Test organisms (species):
Daphnia magna
Details on test organisms:
- Common name: freshwater crustacean, Daphnia magna
- Age at study initiation (mean and range, SD): <24 hours old
- Source: continuous laboratory cultures held at Brixham Environmental Laboratory.

- Acclimation conditions (same as test or not): same
- Type and amount of food: fed on a mixed algae diet of Chlorella vulgaris, strain CCAP 211/12 and Pseudokirchneriella subcapitata, strain CCAP 278/4.
- Feeding frequency: fed daily ad libitum depending on age and density of the culture.
Test type:
Water media type:
Limit test:
Total exposure duration:
48 h
236 mg/L CaCO3
Test temperature:
20 oC
7.1 to 7.9
Dissolved oxygen:
9.0 to 9.6 mg/L
643 uS cm-1
Nominal and measured concentrations:
Nominal concentrations: 0.56, 1.0, 1.8, 3.2, 5.6, 10, 32 and 56 mg/L
Measured concentrations: 0.55, 0.99, 1.8, 3.2, 5.6, 10, 33 and 56 mg/L
Details on test conditions:
- Test vessel: Glass beakers of 250 ml nominal capacity
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume:
- Volume of solution: 200 ml of test solution providing a depth of approximately 60 mm
- Aeration:
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate): no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates):
- Biomass loading rate:

- Adjustment of pH: no
- Photoperiod: 16 hours light: 8 hours dark, with 20 minute dusk and dawn transition periods
- Light intensity:

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
An assessment of the response of the D. magna was made 24 and 48 hours after the start of the test. Each D. magna was viewed by eye and was defined as affected if showing no whole body movement, relative to the water, within a period of 15 seconds even if movement of individual appendages was visible. D. magna so affected were termed immobile. Any overt symptoms of toxicity were also recorded.
Reference substance (positive control):
Key result
48 h
Dose descriptor:
Effect conc.:
18 mg/L
Nominal / measured:
Conc. based on:
test mat.
Basis for effect:
Details on results:
Percentage immobilised at 48 h:
Nominal 32 mg/L: 100%;
Nominal 56 mg/L: 100%
Validity criteria fulfilled:
The 48 hours EC50 of the test item is determined as 18 mg/L.
Executive summary:

The acute toxicity of the test item to Daphnia magna was determined, according to OECD Guideline 202 under GLP. The 48 hours EC50 of the test item is determined as 18 mg/L.

Description of key information

The acute toxicity of the test item to Daphnia magna was determined, according to OECD Guideline 202 under GLP. The 48 hours EC50 of the test item is determined as 18 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Dose descriptor:
Effect concentration:
18 mg/L

Additional information