[No public or meaningful name is available]

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reason / purpose for cross-reference:

read-across source

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

44.35 mg/L

Nominal / measured:

nominal

Conc. based on:

other: Nominal with concentration gradient confirmed by TOC

Basis for effect:

mortality (fish)

Remarks:

sublethal effects, if any, will be noted

Remarks on result:

other: 19.9-98.5 mg/L

Key result

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

33.02 mg/L

Nominal / measured:

nominal

Conc. based on:

other: nominal with concentration gradient confirmed by TOC

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: no sign of sublethal effects observed in controls. No sublethal effects were observed in test treatments during the 72 hour testing period. At the 96 hour exposure period, the sublethal effect of lethargy was observed in one organism in the 0.11 mg/L concentration.

- Other biological observations: no sign of sublethal effects

- Mortality of control: 100 percent survival

- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: Some product was observed on the water surface or on the bottom of the tank at the 3.35, 33.02 and 330 mg/L concentrations

Results with reference substance (positive control):

No reference tox reported

Reported statistics and error estimates:

Results of the toxicity test were interpreted using standard statistical techniques. LC50 values were computed using an EPA (Stephen 1983) computer program that uses the probit, spearman karber, and binomial methods.

Sublethal observations / clinical signs:

Table 1: Survival and Sublethal Effect Data

Test Concentration mg/L	Replicate	Number of Survivors at Hour					Number Affected  at Hour
		0	24	48	72	96	24	48	72	96
0.00	A	10	10	10	10	10	0	0	0	0
0.11	A	10	10	10	10	10	0	0	0	1
0.35	A	10	10	10	9	9	0	0	0	0
3.35	A	10	10	9	9	8	0	0	0	0
33.02	A	10	10	10	10	9	0	0	0	0
330	A	10	0	0	0	0	*	*	*	*

Table 2: Median Lethal Concentration (LC50), Median Effect Concentration (EC50), and No Observed Effect Concentration (NOEC)

Exposure Period (hours)	LC50 (mg/L)	EC50 (mg/L)	NOEC (mg/L)
24	104.69	104.69	33.02
48	83.07	83.07	33.02
72	68.69	68.69	33.02
96	44.35	44.35	33.02

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity of the test material to the freshwater fish, Oncorhynchus my kiss, was investigated and resulted in a 96 hour LC50 of 44.35 mg/L and an NOEC of 33.02 mg/L.

Executive summary:

Introduction. A study was performed to assess the acute toxicity of the test material to rainbow trout using OECD 203 and U.S. EPA 797 Guidelines.

 

Methods. 10 fish per test concentration were exposed to dispersions of the test material in reconstituted water over a range of nominal concentrations of 0, 0.11, 0.35, 3.35, 33.02, and 330 mg/L. TOC analysis was conducted that confirmed the concentration gradient. The test was conducted under static conditions for 96 hours at a temperature of 15C. The number of fish mortalities was recorded after, 24, 48, 72, and 96 hours. 

 

Results The acute toxicity of the test material to the freshwater fish, Oncorhynchus mykiss, was investigated and resulted in a 96 hour LC50 of 44.35 mg/L and an NOEC of 33.02 mg/L. No sublethal effects were observed during the 72 hour testing period in all treatments and controls. The sublethal effect of lethargy was observed in one organism in the 0.11 mg/L test concentration at the 96 hour exposure period.

Description of key information

The acute toxicity of the test material to the freshwater fish, Oncorhynchus mykiss, was investigated and resulted in a 96 hour LC50 of 44.35 mg/L and an NOEC of 33.02 mg/L.  

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

44.35 mg/L

Additional information

A study was performed to assess the acute toxicity of the test material to rainbow trout using OECD 203 and U.S. EPA 797 Guidelines.

 

Methods. 10 fish per test concentration were exposed to dispersions of the test material in reconstituted water over a range of nominal concentrations of 0, 0.11, 0.35, 3.35, 33.02, and 330 mg/L. TOC analysis was conducted that confirmed the concentration gradient. The test was conducted under static conditions for 96 hours at a temperature of 15C. The number of fish mortalities was recorded after, 24, 48, 72, and 96 hours. 

 

Results The acute toxicity of the test material to the freshwater fish,Oncorhynchus my kiss, was investigated and resulted in a 96 hour LC50 of 44.35 mg/L and an NOEC of 33.02 mg/L. No sublethal effects were observed during the 72 hour testing period in all treatments and controls. The sublethal effect of lethargy was observed in one organism in the 0.11 mg/L test concentration at the 96 hour exposure period.