Registration Dossier
Registration Dossier
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EC number: - | CAS number: -
Toxicity to soil microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Duration:
- 28 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 other: mg/kg
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- nitrate formation rate
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 other: mg/kg
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- nitrate formation rate
- Details on results:
- RESULTS
- Absorbance readings and nitrate concentrations determined on each sampling day are given in Table 1 (attached).
- Nitrate formation rates and percentage inhibition of nitrogen transformation activity on Day 28 are given in Table 2 (attached).
VALIDATION CRITERIA
- Variation between replicate control nitrification rates was < 15 % and therefore satisfied the criterion given in the test guideline.
ENVIRONMENTAL MEASUREMENTS
- Temperature was maintained between 19 and 21 °C throughout the test.
- Moisture content of each control and test vessel was adjusted to 45 % of the WHC (± 5 %) of the soil on Days 7, 14, 21 and 28. - Results with reference substance (positive control):
- - Results from the positive control were within the normal ranges for 2-chloro-6-(trichlormethyl)pyridine (see Appendix 3, attached)
- Validity criteria fulfilled:
- yes
- Conclusions:
- The effect of the test item on the nitrogen transformation activity of the soil microorganisms gave an EC50 value of greater than 1000 mg/kg. The No Observed Effect Concentration (NOEC) after 28 days exposure was 1000 mg/kg.
- Executive summary:
GUIDELINE
Nitrogen transformation activity of soil microorganisms was investigated using a method designed to be compatible with OECD Guidelines for Testing of Chemicals (2000) No 216 "Soil Microorganisms: Nitrogen Transformation Test" and Method C.21 of Commission Regulation (EC) No. 440/2008.
METHODS
Soil microorganisms were exposed to the test item at concentrations of 10, 32, 100, 320 and 1000 mg/kg for 28 days at temperatures of between 19 and 21 °C in the dark with the addition of powdered Lucerne-green-grass meal to act as a respiratory substrate.
The inhibitory effort of the test item on nitrogen transformation was assessed by the determination of nitrate concentration in the soil samples on Days 0 and 28 and compared to data obtained from control soil samples.
RESULTS
The effect of the test item on the nitrogen transformation activity of the soil microorganisms gave an EC50 value of greater than 1000 mg/kg. The No Observed Effect Concentration (NOEC) after 28 days exposure was 1000 mg/kg.
Reference
Description of key information
The effect of the test item on the nitrogen transformation activity of the soil microorganisms gave an EC50 value of greater than 1000 mg/kg. The No Observed Effect Concentration (NOEC) after 28 days exposure was 1000 mg/kg (OECD 216 and EU Method C.21).
Key value for chemical safety assessment
- Short-term EC50 for soil microorganisms:
- 1 000 mg/kg soil dw
- Long-term EC10 or NOEC for soil microorganisms:
- 1 000 mg/kg soil dw
Additional information
Nitrogen transformation activity of soil microorganisms was investigated using a method designed to be compatible with OECD Guidelines for Testing of Chemicals (2000) No 216 "Soil Microorganisms: Nitrogen Transformation Test" and Method C.21 of Commission Regulation (EC) No. 440/2008.
Soil microorganisms were exposed to the test item at concentrations of 10, 32, 100, 320 and 1000 mg/kg for 28 days at temperatures of between 19 and 21°C in the dark with the addition of powdered Lucerne-green-grass meal to act as a respiratory substrate.
The inhibitory effort of the test item on nitrogen transformation was assessed by the determination of nitrate concentration in the soil samples on Days 0 and 28 and compared to data obtained from control soil samples.
The effect of the test item on the nitrogen transformation activity of the soil microorganisms gave an EC50 value of greater than 1000 mg/kg. The No Observed Effect Concentration (NOEC) after 28 days exposure was 1000 mg/kg.
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