methyl (2E)-[2-(chloromethyl)phenyl](methoxyimino)acetate

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study with GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2006

Report date:

2006

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

GLP compliance:

yes (incl. QA statement)

Remarks:

BASF AG, Experimental Toxicology and Ecology

Test type:

standard acute method

Limit test:

yes

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

- Name of test material (as cited in study report): 520 F-Chlormethyloximether
- Analytical purity: 94.4g/100g
- Lot/batch No.: 31196-182
- Storage condition of test material: The stability under storage conditions over the study period was guaranteed by the sponsor, and the sponsor holds this responsibility.

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: RCC Ltd Laboratory Animal Services, Woelferstrasse 4, CH-4414 Fuellinsdorf, Switzerland
- Age at study initiation: male animals approx. 8 - 10 weeks, female animals approx. 15 weeks
- Weight at study initiation: male 255 g (251-259), female 213 g (208-224)
- Housing: single
- Diet (e.g. ad libitum): Kliba-Labordiet, Provimi Kuba SA, Kaiseraugst, Basel
- Water (e.g. ad libitum): tap water
- Acclimation period: at least 5 days


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20-24
- Humidity (%): 30-70
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Type of coverage:

semiocclusive

Vehicle:

olive oil

Details on dermal exposure:

TEST SITE
- Area of exposure: 40 cm2
- % coverage: 10


REMOVAL OF TEST SUBSTANCE
- Washing (if done): yes, with warm water
- Time after start of exposure: 24 h


TEST MATERIAL
- Concentration (if solution): 60 g/100 ml


VEHICLE
- Amount(s) applied (volume or weight with unit): 3.33 ml/kg bw

Duration of exposure:

24 h

Doses:

2000 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Individual body weights shortly before application (day 0), weekly thereafter and at the end of the study. Recording of signs and symptoms several times on the day of application, at least once each workday for the individual animals.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, other: Individual readings 30 - 60 minutes after removal of the semiocclusive dressing (day 1), as a rule weekly thereafter and at the end of the study (last day of the observation period). The evaluation of skin reactions was performed according to Draize, J.H. (1959).

Statistics:

The data were statistically evaluated by a probit analysis or a binomial test.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Based on:

test mat.

Mortality:

No mortality occurred

Clinical signs:

No systemic clinical observations were observed during clinical examination.

Body weight:

The mean body weights of the animais increased throughout the study period.

Gross pathology:

No macroscopic pathologic abnormalities were noted in the animals (5 males and 5 females) examined at termination of the study.

Other findings:

- Other observations: No Iocal effects were observed