N,N-diethylaniline

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: data is from authoritative database

Justification for type of information:

data is from authoritative database

Qualifier:

according to guideline

Guideline:

other: refer principle below

Qualifier:

according to guideline

Guideline:

other: "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances"

Principles of method if other than guideline:

BCF value of test chemical is determined in fish Cyprinus carpio for 8 weeks on the basis of whole body weight.

GLP compliance:

not specified

Radiolabelling:

not specified

Details on sampling:

1 w 2 w 4 w 6 w and 8 w

Vehicle:

yes

Remarks:

Name and concentration of vehicle : HCO-40,

Details on preparation of test solutions, spiked fish food or sediment:

PREPARATION AND APPLICATION OF TEST SOLUTION
1st Concentration area : 1.0 ppm, 2nd Concentration area : 0.1 ppm

Test organisms (species):

Cyprinus carpio

Route of exposure:

aqueous

Test type:

not specified

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

8 wk

Details on test conditions:

RANGE-FINDING / PRELIMINARY STUDY - Test concentrations : TLm(48h) 16.8 ppm(w/v), Rice fish (Oryzias latipes)

Nominal and measured concentrations:

- Nominal concentrations 1st Concentration area: 0.2 ppm, 2nd Concentration area: 0.02 ppm

Conc. / dose:

0.2 mg/L

Type:

BCF

Value:

>= 44 - <= 161 L/kg

Basis:

whole body w.w.

Time of plateau:

8 wk

Remarks on result:

other: other details not available

Conc. / dose:

0.02 mg/L

Type:

BCF

Value:

>= 17 - <= 125 L/kg

Basis:

whole body w.w.

Time of plateau:

8 wk

Remarks on result:

other: other details not available

		2 W	4 W	6W	8W
1st Concentration area	Test substance concentration (ppm)	0.166	0.157	0.161	0.162
	Bioconcentration factor (L/kg)	75	95	44	161
		63	118	57	64
2nd Concentration area	Test substance concentration (ppm)	0.021	0.020	0.019	0.020
	Bioconcentration factor (L/kg)	98	57	28	125
		80	17	29	75

Validity criteria fulfilled:

not specified

Conclusions:

The bioconcentration factor (BCF) for test chemical was determined in Cyprinus carpio . The BCF value was observed to be in range 44-161 L/kg at dose concentration 0.2 mg/L and 17-125 L/kg at dose concentration 0.02 mg/L on the basis of total lipid content on test organism Cyprinus carpio during 8 weeks period. Therefore it is concluded that this test chemical is nonbioaccumulative.

Executive summary:

Bioaccumulation test was conducted for 8 weeks for determination the bioconcentration factor (BCF) of chemical on testorganism Cyprinus carpio.

Nominal concentrations used in the study are Nominal concentrations 1st Concentration area: 0.2 ppm(v/v), 2nd Concentration area: 0.02 ppm(v/v) and Range finding study was carried out on Rice fish (Oryzias latipes) TLm(48h) 16.8 ppm(w/v). The vehicle used in this study was HCO-40.The bioconcentration factor (BCF) for test chemical was determined in  Cyprinus carpio . The BCF value was observed to be in range 44-161 L/kg at dose concentration 0.2 mg/L and 17-125 L/kg at dose concentration 0.02 mg/L on the basis of total lipid content on test organism Cyprinus carpio during 8 weeks period. Therefore it is concluded that this test chemical is nonbioaccumulative. These range BCF values indicate that the test chemical is not bioaccumulative in fish and food chain as these values are far less than 2000 criteria.

Description of key information

Bioaccumulation test was conducted for 8 weeks for determination the bioconcentration factor (BCF) of chemical on testorganism Cyprinus carpio.

Nominal concentrations used in the study are Nominal concentrations 1st Concentration area: 0.2 ppm(v/v), 2nd Concentration area: 0.02 ppm(v/v) and Range finding study was carried out on Rice fish (Oryzias latipes)TLm(48h) 16.8 ppm(w/v). The vehicle used in this study was HCO-40.The bioconcentration factor (BCF) for test chemical was determined in  Cyprinus carpio . The BCF value was observed to be in range 44-161 L/kg at dose concentration 0.2 mg/L and 17-125 L/kg at dose concentration 0.02 mg/L on the basis of total lipid content on test organism Cyprinus carpio during 8 weeks period. Therefore it is concluded that this test chemical is nonbioaccumulative.These range BCF values indicate that the test chemical is not bioaccumulative in fish and food chain as these values are far less than 2000 criteria.

Additional information

Various experimental studies and prediction have been reviewed for test chemical for bioaccumulation endpoint in water endpoint and their results are summarized below.

First study was experimental study reviewed from authoritative database ( J -check) in this study the bioaccumulation test was conducted for 8 weeks for determination the bioconcentration factor (BCF) of chemical on test organism Cyprinus carpio. Nominal concentrations used in the study are Nominal concentrations 1st Concentration area: 0.2 ppm(v/v), 2nd Concentration area: 0.02 ppm(v/v) and Range finding study was carried out on Rice fish (Oryzias latipes) TLm(48h) 16.8 ppm(w/v). The vehicle used in this study was HCO-40.The bioconcentration factor (BCF) for test chemical was determined in  Cyprinus carpio . The BCF value was observed to be in range 44-161 L/kg at dose concentration 0.2 mg/L and 17-125 L/kg at dose concentration 0.02 mg/L on the basis of total lipid content on test organism Cyprinus carpio during 8 weeks period. Therefore it is concluded that this test chemical is nonbioaccumulative. These range BCF values indicate that the test chemical is non bioaccumulative in fish and food chain as these values are far less than 2000 criteria.

Another study was reviewed from Ecotoxicology and Environmental Safety journal (1980) in this study the BCF value of test chemical was estimated by using equation taken from Kenaga and Goring (1978). The bioaccumulation concentration factor of test chemical was estimated to be 16 dimensionless by using water solubility of test chemical. On the basis of BCF value it is concluded that test chemical is non bioaccumulative in nature.

In last study prediction was done by using BCFBAF Program (v3.00) model of EPI suite (2018) the estimated bio concentration factor (BCF) for test chemical  is 70.95 L/kg wet-wt at 25 deg. c which does not exceed the bioconcentration threshold of 2000. Therefore it is concluded that test chemical is non-bioaccumulative in food chain.

By considering results of all the studies mentioned above it is concluded that BCF value of test chemical is in range of 16-161 L/Kg this range BCF value indicates that test chemical is non bioaccumulative in nature.