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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Description of key information

The result of the acute toxicity study is questionable. The EC50 determined was 37.2 mg/L. However, the NOEC in the Daphnia reproduction test was 30 mg/L (the highest dose tested) and the acute toxicity results for a structural analogue were EC0, 50 and 100 > 160 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
37.2 mg/L

Additional information

The EC50 for Reactive Red 239 was estimated by extrapolation to be 37.2 mg/L with 95% confidence limits of 32.1 -43.9 mg/L. The EC0 was determined to be 15 mg/L. The EC50 was estimated by extrapolation using Litchfield-Wilcoxon calculation.

The acute toxicity of the structural analogue SA01 on Daphnia magna was assessed in an Acute Immobilisation Test, over an exposure period of 48 hours in a semi-static test system.The frequency of the water renewal periods were 24 hours.

Toxic effects were not observed during the preliminary concentration range-finding test therefore a limit test was performed in the main experiment using only one test concentration (160 mg/L nominal) and one control group. Based on the analytical method validation and the fact that the test substance is not hydrolytically stable, a nominal concentration higher than 100 mg/L was tested, in order to achieve a measured test concentration of 100 mg/L in the limit test.

Only one immobilised animal could be observed in the test item treated group. In addition, no abnormal behaviour or appearance of test animals was detected.

Under the conditions of this Daphnia magnaacute immobilisation study the observed endpoints were the following:


Parameter (48h)

Test item concentration [mg/L]


> 160




> 160


> 160


Based on the results of this study, the test item had no toxic effect on daphnids at up to at least 160 mg/L nominal.