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EC number: 402-420-3 | CAS number: 89157-03-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 987
- Report date:
- 1987
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.12 (Mutagenicity - In Vivo Mammalian Erythrocyte Micronucleus Test)
- Version / remarks:
- 84/449/EWG, B.12
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- GLP compliance:
- yes
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- -
- EC Number:
- 402-420-3
- EC Name:
- -
- Cas Number:
- 89157-03-9
- Molecular formula:
- C31H19ClN7Na5O19S6
- IUPAC Name:
- pentasodium 5-{[4-chloro-6-({4-[2-(sulfonatooxy)ethanesulfonyl]phenyl}amino)-1,3,5-triazin-2-yl]amino}-3-[2-(1,5-disulfonatonaphthalen-2-yl)diazen-1-yl]-4-hydroxynaphthalene-2,7-disulfonate
- Test material form:
- solid: particulate/powder
- Details on test material:
- Reactive Read 239
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- NMRI
- Sex:
- male/female
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- deionised water
- Duration of treatment / exposure:
- animals were killed after 24, 48 or 72 hours after administration of the test compound
- Frequency of treatment:
- The test compound was given in two equal parts within two hours
Doses / concentrations
- Remarks:
- Doses / Concentrations:
6250 mg/kg
Basis:
nominal in water
- No. of animals per sex per dose:
- Male: 6250 mg/kg; No. of animals: 5; Sacrifice time: 24 hours
Male: 6250 mg/kg; No. of animals: 5; Sacrifice time: 48 hours
Male: 6250 mg/kg; No. of animals: 5; Sacrifice time: 72 hours
Female: 6250 mg/kg; No. of animals: 5; Sacrifice times: 24 hours
Female: 6250 mg/kg; No. of animals: 5; Sacrifice times: 48 hours
Female: 6250 mg/kg; No. of animals: 5; Sacrifice times: 72 hours - Positive control(s):
- endoxane, 50 mg/kg BW
Results and discussion
Test results
- Key result
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- no effects
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- The incidence of micronucleated polychromatic erythrocytes in the dose groups of Reaktiv-Rot F-52167 FW was within the normal range of the negative control groups. No statistically significant increase of micronucleated polychromatic erythrocytes has been observed.
Due to the colour of the test compound urine, faeces and skin were red coloured.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
not mutagenic - Executive summary:
Reactive Red 239 was tested in the micronucleus test. The test compound was administered orally by gavage to male and female mice. Doses levels of 0 and 6250 mg per kg bodyweight were tested. The 6250 mg per kg bodyweight dose level was chosen since a preliminary study had shown it to be the maximal applicable dose. The test compound was given in two equal parts within two hours and according to the test procedure the animals were killed 24, 48, 72 hours after administration of the test compound.
Endoxan was used as positive control substance and was administered orally at a dose of 50 mg per kg bodyweight.
The incidence of micronucleated polychromatic erythrocytes of the animals treated with Reactive Red 239 was within the normal range of the negative control. The number of normochromatic erythrocytes containing micronuclei was not increased. The ratio of polychromatic/normochromatic erythrocytes in both male and female animals remained unaffected by the treatment with Reactive Red 239 and was statistically not different form the control values.
Endoxan induced a marked statistically significant increase in the number of polychromatic cells with micronuclei in both males and females, indicating the sensitivity of the system. The ratio of polychromatic erythrocytes to normocytes was not changed to a significant extend.
The results indicated that, under the conditions of the present study, Reactive Red 239 is not cytogenic in the micronucleus test.
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