Quaternary ammonium compounds, tri-C8-10-alkylmethyl, chlorides

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

17 Oct - 12 Dec 1989

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions (non GLP, test substance not characterized in detail).

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

yes

Remarks:

non GLP, test substance not characterized in detail

GLP compliance:

no

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Quaternary ammonium compounds, tri-C8-10-alkylmethyl, chlorides

EC Number:

264-120-7

EC Name:

Quaternary ammonium compounds, tri-C8-10-alkylmethyl, chlorides

Cas Number:

63393-96-4

Molecular formula:

Unspecified

IUPAC Name:

Quaternary ammonium compounds, tri-C8-10-alkylmethyl, chlorides

Details on test material:

- Physical state: yellowbrown, oily liquid
- Purity test date: not specified

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Winkelmann, Borchen
- Fasting period before study: 16 h
- Housing: 5 animals per cage in Makrolon 3 cages
- Diet: Altromin-Haltungsdiät 1324, Fa. Altromin, ad libitum
- Water: tap water, ad libitum
- Acclimation period: 7-8 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 - 25
- Humidity (%): 45 - 60
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

peanut oil

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 20% (g/V) for 2000 mg/kg bw and 2% (g/V) for 200 mg/kg bw

MAXIMUM DOSE VOLUME APPLIED: 10 mL/kg

Doses:

200, 2000 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: observations for clinical signs and mortality regularly on day of application and 2 x daily thereafter, weighing was carried out one day before application, on day of application and 2, 7 and 14 days following application
- Necropsy of survivors performed: yes

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 200 - < 2 000 mg/kg bw

Based on:

test mat.

Remarks on result:

other: no substance related death at 200 mg/kg bw, but all animals died at the highest dose of 2000 mg/kg bw

Mortality:

Neither female nor male animals died in the 200 mg/kg bw test group. Only one male animal had to be killed after 52 hours, but this was due to mistake during gavage. In the 2000 mg/kg bw test group 4 male animals and all female animals died within the first 30 hours after dosing. The additional male animal died 48 hours after application. Thus, mortality was 100% at 2000 mg/kg bw.

Clinical signs:

other: Female animals dosed with 200 mg/kg bw did not show any clinical signs. The one male animal that died due to gavage error showed decreased activity, brownish colored nose, vocalization, diarrhoea, severe reduction of body weight and chromodacryorrhoea but

Gross pathology:

For the animals dosed with 200 mg/kg bw no abnormal findings at gross necropsy were detected. 2 male animals dosed with 2000 mg/kg bw showed a severe gaseous distention of the small intestine and all 5 female animals showed a slight to severe gaseous distention of the small intestine and the stomach. 1 female animal had a red discolored glandular stomach and the duodenum filled with liquid. 2 male animals and all 5 female animals showed a severe hydrothorax, 2 male animals had redness of the fundus area and moderate ascites. 1 male animal had a filled small intestine, slight ascites and a slight hypothorax.

Any other information on results incl. tables

Table 1. Table for acute oral toxicity. 

Dose [mg/kg bw]	Toxicological results*	Duration of clinical signs	Time of death	Mortality (%)
Males
200	1/1/5	Day 1	---	20
2000	5/5/5	Day 1	24-48 h	100
Females
200	0/0/5	Day 1	---	0
2000	5/5/5	Day 1	24-28 h	100
LD50 = 174 mg/kg bw

* first number = number of dead animals                                 

 second number = number of animals with clinical signs         

 third number = number of animals used