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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1991-03-12 to 1991-06-19
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: OECD Guideline study under GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report date:
1991

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
1989
GLP compliance:
yes

Test material

Constituent 1
Reference substance name:
Silicophosphonat
IUPAC Name:
Silicophosphonat
Details on test material:
- Name of test material (as cited in study report): Silicophosponat
- Physical state: Liquid

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Concentrations: 12.5 - 100 mg/L

Test solutions

Details on test solutions:
Stock solution: 100 mg/L in test medium

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Strain: Strauß
- Source: National de Recherche Chimique Appliquee 1978, France, cultured in the laboratory
- Age at study initiation (mean and range, SD): 2-24 hours
- Feeding during test: none

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
none

Test conditions

Hardness:
Total hardness: 2.7 +/- 0.5 mmol/L
Test temperature:
20 +/- 1 °C
pH:
7.5 - 8.5
Dissolved oxygen:
8.9-9.2 mg O2/L
Nominal and measured concentrations:
Noiminal 12.5, 25, 50, 100 mg/L
Range of the percentage of the nominal concentrations after 48h (Derived from phosphorous): 105-112%
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 20-mL-glass tubes, 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 1-2 animals/mL

TEST MEDIUM / WATER PARAMETERS: Synthetic medium M4

OTHER TEST CONDITIONS
- Photoperiod: 16 h light, 8 h dark
-Light intensity: 5-6 µE/m*m*s (400-700 nm)



EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : EC100/EC50/EC0 (3, 6, 24, 48 h)
Those animals were considered to be immobile which were unable to swim within 15 seconds after gentle agitation of the test vessel.


Reference substance (positive control):
not specified

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Duration:
3 h
Dose descriptor:
EC0
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Duration:
6 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Duration:
6 h
Dose descriptor:
EC0
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Duration:
24 h
Dose descriptor:
EC0
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Details on results:
control: immobilization <10%

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Silicophosphonate is not toxic to the water flea Daphnia magna under the reported test conditions.
Executive summary:

In a 48 h static acute toxicity tests to daphnia according to no significant effects were seen up to a concentration of 100 mg/L. The EC0 is 100mg/L. Both the EC50 and EC100 are greater 100 mg/L. Thus silicophosphonate is not toxic to the water flea Daphnia magna under the reported test conditions.