Silsesquioxanes, 3-phosphonopropyl, Et Me esters, sodium salts

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1991-03-18 to 1991-03-21

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: OECD Guideline study under GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1991

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.3 (Algal Inhibition test)

Version / remarks:

May 1988

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Silicophosphonat

IUPAC Name:

Silicophosphonat

Details on test material:

- Name of test material (as cited in study report): Silicophosphonat
- Physical state:Liquid

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 12.5, 25, 50, 100 mg/L and control

Test solutions

Vehicle:

no

Details on test solutions:

- Stocksolution 200 mg/L

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Strain: CHODAT SAG 86.81
- Source: Collection of algal cultures in Göttingen (SAG
- Method of cultivation: Sterile nutrient medium

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Hardness:

not available

Test temperature:

23 +/- 2 °C

pH:

8

Dissolved oxygen:

not given in the report

Nominal and measured concentrations:

Nominal: 0, 12.5, 25, 50, 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 250 ml Erlenmeyer
- Aeration: not given
- Initial cells density: 10000 cells /mL
- Control end cells density: only given as flourescence pararameter
- No. of vessels per concentration (replicates): 3 inoculated and 1 uninoculated replicate for pH mesurements
- No. of vessels per control (replicates): 3




GROWTH MEDIUM
- Standard medium used: yes


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionised water
- Culture medium different from test medium: no
- Intervals of water quality measurement: not given


OTHER TEST CONDITIONS
- Sterile test conditions: not given
- Adjustment of pH: no
- Photoperiod: permanent illumination
- Light intensity and quality: 120 µEinstein/m*m*s



TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Basis for effect:

biomass

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Basis for effect:

growth rate

Effect concentrations 3

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

> 100 mg/L

Remarks on result:

other: 95 % significance level

Effect concentrations 4

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

> 100 mg/L

Details on results:

- Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): no
- Any stimulation of growth found in any treatment: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: none

Results with reference substance (positive control):

-

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The substance was not toxic to algea under the test conditions.

Executive summary:

An Algal Growth Inhibition test was performed with the test substance Silicophosphonat. There were neither effects on the development of biomass nor on the growth rate. The no observed effect concentration and the lowest observed effect concentration at 72 h were greater than 100 mg/L.