.beta.-D-Ribofuranose, 5-deoxy-, 1,2,3-triacetate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

15 November 2012 to 26 September 2013

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Fully GLP compliant and in accordance with current test guidelines

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2013

Report date:

2013

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Acetylfuranoside

IUPAC Name:

Acetylfuranoside

Constituent 2

Reference substance name:

ß-D-Ribofuranose, 5-deoxy-, triacetate (6CI,9CI)

EC Number:

612-957-7

Cas Number:

62211-93-2

Molecular formula:

C11 H16 O7

IUPAC Name:

ß-D-Ribofuranose, 5-deoxy-, triacetate (6CI,9CI)

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

Name: Acetylfuranoside (also known as ß-D-Ribofuranose, 5-deoxy-, 1,2,3-triacetate)
CAS number: 62211-93-2
Batch number: 201203272009
Quantity received: 1.2 kg
Purity: 98.7%
Expiry date: 31 Mar 2013
Date of receipt: 14 September 2012
Storage details: When not in use the test article was stored in a sealed container, at room temperature (15 to 30°C) in the dark.

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

After 24 and 48 hours, the Daphnia magna in each test vessel were observed for evidence of immobility.
At the start of the test (0 hours) and media renewal at 24 hours, duplicate 100 mL samples of freshly prepared test media were taken from the control and 150 mg/L test media preparation flask for chemical analysis.
At 24 and 48 hours, duplicate 100 mL samples were taken for chemical analysis from the pooled expired test media from the 150 mg/L test concentration and control.

Test solutions

Vehicle:

yes

Details on test solutions:

The range-finding test was conducted at nominal test substance concentrations of 0.1, 1.0, 10 and 100 mg/L under semi-static test conditions. A control group was also included. Duplicate test vessels were prepared for the control and each test substance concentration. Based on nominal concentrations, the results of the range-finding test suggested that the 48-hour EC50 value would be greater than 100 mg/L.
Based on the results of the range-finding test which is not fully reported, the definitive/limit test was conducted under semi-static conditions at nominal concentrations of 0 (control) and 150 mg/L.
The test vessels (nominal volume 150 mL) were glass crystallising dishes covered with clear plastic petri dish lids. Four replicate test vessels were prepared for the control and each test concentration.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Source: Smithers Viscient, Shawbury, UK
- Age at study initiation (mean and range, SD): less than 24 hours old
- Weight at study initiation (mean and range, SD): Not applicable
- Length at study initiation (length definition, mean, range and SD): Not applicable
- Method of breeding: New cultures were started with juvenile Daphnia magna (less than 24 hours old), at a density of approximately 20 animals per litre. The cultures were fed daily with a concentrated suspension of Chlorella vulgaris prepared in accordance with local standard operating procedures. A seaweed extract was also added at each medium renewal as a dietary supplement.
- Feeding during test: Not fed during test
- Food type: Not applicable
- Amount: Not applicable
- Frequency: Not applicable

ACCLIMATION
- Acclimation period: Not applicable

Study design

Test type:

semi-static

Water media type:

not specified

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

Total hardness as CaCO3 (mg/L) control at 0 hours = 145 mg/L and 24 hours = 149 mg/L

Test temperature:

Temperature range: 20.7 - 21.0°C
Temperature control vessel: 0 hour - 20.0°C, 24 hours (old) - 20.0°C, 24 hours (new) - 20.6°C, 48 hours (old) - 19.5°C
Temperature 125 mg/L test solution: 0 hour - 20.6°C, 24 hours (old) - 20.2°C, 24 hours (new) - 21.5°C, 48 hours (old) - 19.7°C

pH:

pH control vessel: 0 hour (new) - 7.58, 24 hours (old) - 8.25, 24 hours (new) - 7.73, 48 hours (old) - 8.14
pH 125 mg/L test solution: 0 hour (new) - 7.75, 24 hours (old) - 7.94, 24 hours (new) - 7.86, 48 hours (old) - 7.88

Dissolved oxygen:

Dissolved oxygen % ASV (mg/L)
Control vessel: 0 hour (new) - 99 (9.13), 24 hours (old) - 97 (8.90), 24 hours (new) - 98 (8.99), 48 hours (old) - 95 (8.87)
125 mg/L test solution: 0 hour (new) - 99 (9.15), 24 hours (old) - 95 (8.75), 24 hours (new) - 99 (8.74), 48 hours (old) - 95 (8.81)

Nominal and measured concentrations:

Nominal concentration: 150 mg/L
0 hours (new): 139 mg/L (93% nominal)
24 hours (new): 131 mg/L (87% nominal)
24 hours (old): 115 mg/L (77% nominal)
48 hours(old): 115 mg/L (77% nominal)
Mena measured concentration: 125 mg/L (83% nominal)

Details on test conditions:

TEST SYSTEM
- Test vessel: The test vessels (nominal volume 150 mL) were glass crystallising dishes covered with clear plastic petri dish lids.
- Fill volume: 100 mL
- Aeration: Not applicable
- Renewal rate of test solution (frequency/flow rate): 24 hours
- No. of organisms per vessel: 5 juveniles
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ASTM
- Intervals of water quality measurement: At the end of each exposure period

OTHER TEST CONDITIONS
- Adjustment of pH: Determined at start and each of each test (see pH section)
- Photoperiod: Fluorescent lighting was supplied on a 16-hour light: 8 hour dark day length
- Light intensity: Not reported

EFFECT PARAMETERS MEASURED: After 24 and 48 hours, the Daphnia magna in each test vessel were observed for evidence of immobility. The observations differentiated between mobile and immobile daphnids. An individual was considered immobile if, when the contents of the test vessel were briefly agitated, it did not swim during a 15-second period of observation. In addition, Daphnia magna submerged in the body of the test media and those that were held at the surface of the test media were also recorded.

TEST CONCENTRATIONS
- Range finding study: The range-finding test was conducted at nominal test substance concentrations of 0.1, 1.0, 10 and 100 mg/L under semi-static test conditions. A control group was also included. Duplicate test vessels were prepared for the control and each test substance concentration. Based on nominal concentrations, the results of the range-finding test suggested that the 48-hour EC50 value would be greater than 100 mg/L.
- Test concentrations: Based on the results of the range-finding test which is not fully reported, the definitive/limit test was conducted under semi-static conditions at nominal concentrations of 0 (control) and 150 mg/L.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

125 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 125 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

Chemistry data
The limit of detection (LOD) for Acetylfuranoside in ASTM medium using this method was 0.014 mg/L.
The analytical results showed that on preparation at 0-hour and 24-hours, Acetylfuranoside achieved a concentration of 139 mg/L and 131 mg/L (93% and 87% of nominal concentration, respectively). At the 24 hour and 48-hour exposure periods the recovery dropped to 115 mg/L (77% of nominal concentrations). The time weighted mean calculation showed that Acetylefuranoside achieved a time weighted mean measured concentration of 125 mg/L (83% of nominal concentration).

Test media descriptions
The test preparations were observed to be colourless solutions throughout the duration of the test.

Toxicity to daphnia
After 48-hours, the concentration at which no statistically significant dose related immobility occurred was 125 mg/L and therefore the NOEC was considered to be 125 mg/L.
In terms of nominal concentrations the 48-hour EC50 value was determined to be greater than 125 mg/L.
It is concluded that Acetylfuranoside was considered not toxic to Daphnia magna at nominal concentrations less than 125 mg/L under the conditions of this test.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 48-hour acute toxicity of Acetylfuranoside to the freshwater planktonic crustacean, Daphnia magna, was determined in accordance with the requirements of OECD Chemicals Testing Guideline No. 202 Daphnia sp. Acute Immobilisation Test (adopted April 13 2004).
The validity criteria for control immobility (=10%) and dissolved oxygen (>60% ASV) were both satisfied and the test is therefore considered valid.
It is concluded that Acetylfuranoside was considered not toxic to Daphnia magna at nominal concentrations less than 125 mg/L under the conditions of this test.

Executive summary:

The objective of the study was to determine the 48-hour acute toxicity of Acetylfuranoside to the freshwater planktonic crustacean, Daphnia magna. The study was conducted in accordance with the requirements of OECD Chemicals Testing Guideline No. 202 Daphnia sp. Acute Immobilisation Test (adopted April 13 2004).

Based on the results of a range-finding test which are not fully reported, the definitive/limit test was conducted semi-statically at a nominal concentration of 150 mg/L. A control group was also included.

At the start of the test, five juvenile Daphnia magna were added to each test vessel. The Daphnia magna in each test vessel were observed at 24 and 48 hours. The Daphnia magna were not fed during the test.

The analytical results showed that on preparation at 0-hour and 24-hours, Acetylfuranoside achieved a concentration of 139 mg/L and 131 mg/L (93% and 87% of nominal concentration, respectively).  At the 24-hour and 48-hour exposure periods the recovery dropped to 115 mg/L (77% of nominal concentrations).  The time weighted mean calculation showed thatAcetylfuranosideachieved a time weighted mean measured concentration of 125 mg/L (83% of nominal concentration).

The [1] ECx values and the corresponding [2] NOEC values are presented in the following table. Values are presented in terms of time weighted mean measured concentrations.

		Nominal Concentration (mg/L)
		24 hours	48 hours
EC50		>125	>125
NOEC		125	125
(x-x)	95% Confidence Limits

After 48-hours, the concentration at which no statistically significant dose related immobility occurred was 125 mg/L and therefore the NOEC was considered to be 125 mg/L.

In terms of nominal concentrations the 48-hour EC₅₀ value was determined to be greater than 125 mg/L.

The validity criteria for control immobility (=10%) and dissolved oxygen (>60% ASV) were both satisfied and the test is therefore considered valid.

It is concluded that Acetylfuranoside was considered non toxic to Daphnia magna at nominal concentrations less than 125 mg/L under the conditions of this test.

 

[1] Concentration resulting in 50% immobility of exposed Daphnia magna

[2] No observed effect concentration