3,3'-(1,4-Phenylene)bis(5,6-diphenyl-1,2,4-triazine)

Eye irritation

Administrative data

Endpoint:

eye irritation

Remarks:

other: rabbit cornea

Type of information:

experimental study

Adequacy of study:

key study

Study period:

?

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study performed according to GLP and French Official journal method (1999)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other:

Deviations:

no

Principles of method if other than guideline:

Irritant potentiel determined by direct application of the substance on rabbit cornea fibroblast momolayer, by release of Neutral Red.

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

3-[4-(5,6-diphenyl-1,2,4-triazin-3-yl)phenyl]-5,6-diphenyl-1,2,4-triazine

EC Number:

700-823-1

Cas Number:

55514-22-2

Molecular formula:

C36H24N6

IUPAC Name:

3-[4-(5,6-diphenyl-1,2,4-triazin-3-yl)phenyl]-5,6-diphenyl-1,2,4-triazine

Details on test material:

- Name of test material (as cited in study report): WP30
- Physical state: powder
- Analytical purity: 99.6 %
- Purity test date: 23rd December 2008
- Lot/batch No.: LP110
- Stability under test conditions: stable
- Storage condition of test material: at room temperature in a dry and cool place

Test animals / tissue source

Species:

rabbit

Strain:

not specified

Test system

Vehicle:

other: Paraffinic oil

Details on study design:

WP30 was diluted at 10, 25, and 50 % in paraffinic oil for testing. Pure WP30 was tested also. Contact with test system was 60 seconds. A positive reference (SDS) was tested at 0.01, 0.05 and 0.2 %. Neutral red retention was measured after end of contact.
Test system was Predisafe kit:
cell line from Rabbit cornea
cell thawing: vials were thawed in a 37 °C water bath,. Cells were seeded in a 175 cm2 flask which was then placed in a water saturated incubator (0% CO2 and 100% air) during 6 or 7 days with culture medium renewal every 2 or 3 days.
cell seeding: same conditions, during 5 days.

Results and discussion

In vivo

Irritant / corrosive response data:

The highest cell mortality observed was at 50% concentration and was 3.5 %. This negligible cytotoxicity observed allows concluding lack of potential for rabbit cornea irritation.

Any other information on results incl. tables

Neutral red retention in controls was 1.022 (validity threshold: > 0.8)

The positive reference SDS gave CI50 > 0.01 % and < 0.2 %, that is within the acceptability range.

Applicant's summary and conclusion

Interpretation of results:

not irritating

Remarks:

Migrated information Criteria used for interpretation of results: expert judgment

Conclusions:

The negligible cytotoxicity observed allows concluding lack of potential for rabbit cornea irritation.